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Research Output 2005 2020

2019

Expression and purification of recombinant G protein-coupled receptors: A review

Wiseman, D. N., Otchere, A., Patel, J. H., Uddin, R., Pollock, N. L., Routledge, S. J., Rothnie, A. J., Slack, C., Poyner, D. R., Bill, R. M. & Goddard, A. D., 31 Oct 2019, In : Protein Expression and Purification. 167, 105524.

Research output: Contribution to journalArticle

Open Access
File
Drug Design
Drug Discovery
G-Protein-Coupled Receptors
Recombinant Proteins
Detergents

Liposomes as models for membrane integrity

Routledge, S. J., Linney, J. A. & Goddard, A. D., 28 Jun 2019, In : Biochemical Society Transactions. 47, 3, p. 919-932 14 p.

Research output: Contribution to journalReview article

Liposomes
Cell Membrane
Cell membranes
Membranes
Molecules
2018

Dynamic tuneable G protein-coupled receptor monomer-dimer populations

Dijkman, P. M., Castell, O. K., Goddard, A., Munoz-Garcia, J. C., de Graaf, C., Wallace, M. I. & Watts, A., 27 Apr 2018, In : Nature Communications. 9, 1710.

Research output: Contribution to journalArticle

Open Access
File
G-Protein-Coupled Receptors
Dimers
monomers
Monomers
dimers

Mechanistic and phenotypic studies of bicarinalin, BP100 and colistin action on Acinetobacter baumannii

Eales, M. G., Ferrari, E., Goddard, A., Lancaster, L., Sanderson, P. & Miller, C., 8 May 2018, In : Research in Microbiology.

Research output: Contribution to journalArticle

Open Access
File
Colistin
Acinetobacter baumannii
Biofilms
Peptides
Acinetobacter Infections

Recombinant Membrane Protein Methods

Bill, R. M., Goddard, A. D. & Rothnie, A. J., 1 Sep 2018, In : Methods. 147, p. 1-2 2 p.

Research output: Contribution to journalEditorial

Single molecule fluorescence for membrane proteins

Castell, O. K., Dijkman, P. M., Wiseman, D. & Goddard, A., 30 May 2018, In : Methods.

Research output: Contribution to journalArticle

Open Access
File
Membrane Proteins
Fluorescence
Molecules
Photobleaching
Oligomerization
2017

Neurotensin receptor 1 facilitates intracellular and transepithelial delivery of macromolecules

Bird, J. L., Simpson, R., Vllasaliu, D. & Goddard, A. D., 1 Oct 2017, In : European Journal of Pharmaceutics and Biopharmaceutics. 119, p. 300-309 10 p.

Research output: Contribution to journalArticle

Open Access
File
Neurotensin
Caco-2 Cells
Ligands
HEK293 Cells
Eukaryotic Cells

The Paracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanisms

Goddard, A. D., Bali, S., Mavridou, D. A. I., Luque-Almagro, V. M., Gates, A. J., Roldán, M. D., Newstead, S., Richardson, D. J. & Ferguson, S. J., Jan 2017, In : Molecular Microbiology. 103, 1, p. 117-133 17 p.

Research output: Contribution to journalArticle

Open Access
File
Paracoccus denitrificans
Nitrites
Nitrates
Symporters
Nitrate Reductase
2015

Reconstitution of membrane proteins: a GPCR as an example

Goddard, A. D., Dijkman, P. M., Adamson, R. J., dos Reis, R. I. & Watts, A., 2015, In : Methods in Enzymology. 556, p. 405-424 20 p.

Research output: Contribution to journalReview article

Open Access
File
G-Protein-Coupled Receptors
Membrane Proteins
Lipids
Detergents
Cell membranes
2013

Divergence of Erv1-associated mitochondrial import and export pathways in trypanosomes and anaerobic protists

Basu, S., Leonard, J. C., Desai, N., Mavridou, D. A. I., Tang, K. H., Goddard, A. D., Ginger, M. L., Lukeš, J. & Allen, J. W. A., Feb 2013, In : Eukaryotic Cell. 12, 2, p. 343-355 13 p.

Research output: Contribution to journalArticle

Trypanosomiasis
Cytochromes c
Yeasts
Trichomonas
Entamoeba

Lipid-dependent GPCR dimerization

Goddard, A. D., Dijkman, P. M., Adamson, R. J. & Watts, A., 2013, Receptor-receptor interactions. Conn, P. M. (ed.). London (UK): Academic Press, Vol. 117. p. 341-357 17 p. (Methods in Cell Biology; vol. 117).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Dimerization
G-Protein-Coupled Receptors
Lipids
Energy Transfer
Lipid Bilayers
2012

Contributions of fluorescence techniques to understanding G protein-coupled receptor dimerisation

Goddard, A. D. & Watts, A., Dec 2012, In : Biophysical Reviews. 4, 4, p. 291-298 8 p.

Research output: Contribution to journalReview article

Dimerization
G-Protein-Coupled Receptors
Fluorescence
Membrane Proteins
Cell Membrane

Regulation of G protein-coupled receptors by palmitoylation and cholesterol

Goddard, A. D. & Watts, A., 19 Mar 2012, In : BMC Biology. 10, 3 p., 27.

Research output: Contribution to journalComment/debate

Open Access
File
Lipoylation
G-Protein-Coupled Receptors
Cholesterol
cholesterol
protein
2011

A composite biochemical system for bacterial nitrate and nitrite assimilation as exemplified by Paracoccus denitrificans

Gates, A. J., Luque-Almagro, V. M., Goddard, A. D., Ferguson, S. J., Roldán, M. D. & Richardson, D. J., 1 May 2011, In : Biochemical Journal. 435, 3, p. 743-753 11 p.

Research output: Contribution to journalArticle

Open Access
Paracoccus denitrificans
Nitrites
Nitrates
Composite materials
NAD

Cytochrome c biogenesis System I

Stevens, J. M., Mavridou, D. A. I., Hamer, R., Kritsiligkou, P., Goddard, A. D. & Ferguson, S. J., Nov 2011, In : FEBS journal. 278, 22, p. 4170-4178 9 p.

Research output: Contribution to journalReview article

Open Access
Cytochromes c
Heme
Cytochrome c Group
Mitochondria
Proteins

DNA-templated protein arrays for single-molecule imaging

Selmi, D. N., Adamson, R. J., Attrill, H., Goddard, A. D., Gilbert, R. J. C., Watts, A. & Turberfield, A. J., 9 Feb 2011, In : Nano Letters. 11, 2, p. 657-660 4 p.

Research output: Contribution to journalLetter

DNA
deoxyribonucleic acid
proteins
Proteins
Imaging techniques

Oxidation state-dependent protein-protein interactions in disulfide cascades

Mavridou, D. A. I., Saridakis, E., Kritsiligkou, P., Goddard, A. D., Stevens, J. M., Ferguson, S. J. & Redfield, C., 15 Jul 2011, In : Journal of Biological Chemistry. 286, 28, p. 24943-24956 14 p.

Research output: Contribution to journalArticle

Disulfides
Thioredoxins
Oxidation
Protein folding
Periplasm

Solution- and solid-state NMR studies of GPCRs and their ligands

Tapaneeyakorn, S., Goddard, A. D., Oates, J., Willis, C. L. & Watts, A., Jun 2011, In : BBA -Biomembranes. 1808, 6, p. 1462-1475 14 p.

Research output: Contribution to journalReview article

G-Protein-Coupled Receptors
Nuclear magnetic resonance
Ligands
Drug Discovery
Cell signaling
2010

Comparing the substrate specificities of cytochrome c biogenesis Systems I and II

Goddard, A. D., Stevens, J. M., Rondelet, A., Nomerotskaia, E., Allen, J. W. A. & Ferguson, S. J., Feb 2010, In : FEBS journal. 277, 3, p. 726-737 12 p.

Research output: Contribution to journalArticle

Substrate Specificity
Cytochromes c
Heme
Cytochrome c Group
Cytochromes

c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE

Goddard, A. D., Stevens, J. M., Rao, F., Mavridou, D. A. I., Chan, W., Richardson, D. J., Allen, J. W. A. & Ferguson, S. J., 23 Jul 2010, In : Journal of Biological Chemistry. 285, 30, p. 22882-22889 8 p.

Research output: Contribution to journalArticle

Open Access
File
Desulfovibrio desulfuricans
Cytochrome c Group
Mitochondria
Covalent bonds
Cytochromes c
2009

Control of periplasmic interdomain thiol: disulfide exchange in the transmembrane oxidoreductase DsbD

Mavridou, D. A. I., Stevens, J. M., Goddard, A. D., Willis, A. C., Ferguson, S. J. & Redfield, C., 30 Jan 2009, In : Journal of Biological Chemistry. 284, 5, p. 3219-3226 8 p.

Research output: Contribution to journalArticle

Open Access
File
Sulfhydryl Compounds
Disulfides
Oxidoreductases
Reducing Agents
Cysteine

The cationic region of Rhes mediates its interactions with specific Gβ subunits

Hill, C., Goddard, A., Ladds, G. & Davey, J., Feb 2009, In : Cellular Physiology and Biochemistry. 23, 1-3, p. 1-8 8 p.

Research output: Contribution to journalArticle

Open Access
File
GTP-Binding Proteins
Monomeric GTP-Binding Proteins
Heterotrimeric GTP-Binding Proteins
Sequence Homology
G-Protein-Coupled Receptors
2008

Interdependence of two NarK domains in a fused nitrate/nitrite transporter

Goddard, A. D., Moir, J. W. B., Richardson, D. J. & Ferguson, S. J., Nov 2008, In : Molecular Microbiology. 70, 3, p. 667-681 15 p.

Research output: Contribution to journalArticle

Nitrites
Nitrates
Paracoccus denitrificans
Symporters
Protons
2007

Differential effects of RGS proteins on Gαq and Gα11 activity

Ladds, G., Goddard, A., Hill, C., Thornton, S. & Davey, J., 1 Jan 2007, In : Cellular Signalling. 19, 1, p. 103-113 11 p.

Research output: Contribution to journalArticle

Open Access
RGS Proteins
Yeasts
Heterotrimeric GTP-Binding Proteins
Pheromones
Guanosine Triphosphate
2006

Identification of Gnr1p, a negative regulator of Gα signalling in Schizosaccharomyces pombe, and its complementation by human Gβ subunits

Goddard, A., Ladds, G., Forfar, R. & Davey, J., Dec 2006, In : Fungal Genetics and Biology. 43, 12, p. 840-851 12 p.

Research output: Contribution to journalArticle

Schizosaccharomyces
Pheromones
G-Protein-Coupled Receptors
Heterotrimeric GTP-Binding Proteins
Two-Hybrid System Techniques
2005
Galactosidases
galactosidases
Schizosaccharomyces
Schizosaccharomyces pombe
Reporter Genes

Functional analysis of heterologous GPCR signalling pathways in yeast

Ladds, G., Goddard, A. & Davey, J., Jul 2005, In : Trends in Biotechnology. 23, 7, p. 367-373 7 p.

Research output: Contribution to journalReview article

Functional analysis
G-Protein-Coupled Receptors
Yeast
Yeasts
Proteins