1994 …2020

Research output per year

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Research Output

2020

Ligand-induced conformational changes in a SMALP-encapsulated GPCR.

Routledge, S. J., Jamshad, M., Little, H. A., Lin, Y. P., Simms, J., Thakker, A., Spickett, C. M., Bill, R. M., Dafforn, T. R., Poyner, D. R. & Wheatley, M., 1 Jun 2020, In : Biochimica et Biophysica Acta - Biomembranes. 1862, 6, 183235.

Research output: Contribution to journalArticle

Open Access
File

Modification of proteins by reactive lipid oxidation products and biochemical effects of lipoxidation

Spickett, C. & Pitt, A., 2020, In : Essays in Biochemistry. 64, 1, 9–31.

Research output: Contribution to journalReview article

Oxidized phospholipid signaling: Distress to eustress

Spickett, C. M., 2020, Oxidative Stress. Elsevier, p. 263-285

Research output: Chapter in Book/Report/Conference proceedingChapter

2019

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

Teo, A. C. K., Lee, S. C., Pollock, N. L., Stroud, Z., Hall, S., Thakker, A., Pitt, A. R., Dafforn, T. R., Spickett, C. M. & Roper, D. I., 12 Feb 2019, In : Scientific Reports. 9, 1, 1813.

Research output: Contribution to journalArticle

Open Access
File

Epitope mapping and characterization of 4-hydroxy-2-nonenal modified-human serum albumin using two different polyclonal antibodies

Campos-pinto, I., Méndez, L., Schouten, J., Wilkins, J., Fedorova, M., Pitt, A., Davis, P. & Spickett, C., 20 Nov 2019, In : Free Radical Biology and Medicine. 144, p. 234-244 11 p.

Research output: Contribution to journalArticle

Open Access
File

Evaluation of air oxidized PAPC: A multi laboratory study by LC-MS/MS

Ni, Z., Sousa, B. C., Colombo, S., Afonso, C. B., Melo, T., Pitt, A. R., Spickett, C. M., Domingues, P., Domingues, M. R., Fedorova, M. & Criscuolo, A., 20 Nov 2019, In : Free Radical Biology and Medicine. 144, p. 156-166 11 p.

Research output: Contribution to journalArticle

Open Access
File

Increased production of 27-hydroxycholesterol in human colorectal cancer advanced stage: Possible contribution to cancer cell survival and infiltration

Rossin, D., Dias, I. H. K., Solej, M., Milic, I., Pitt, A. R., Iaia, N., Scoppapietra, L., Devitt, A., Nano, M., Degiuli, M., Volante, M., Caccia, C., Leoni, V., Griffiths, H. R., Spickett, C. M., Poli, G. & Biasi, F., 20 May 2019, In : Free Radical Biology and Medicine. 136, p. 35-44 10 p.

Research output: Contribution to journalArticle

Open Access
File

Lipoproteins as targets and markers of lipoxidation

Afonso, C. B. & Spickett, C. M., 1 May 2019, In : Redox Biology. 23, 101066.

Research output: Contribution to journalArticle

Open Access
File

PARP1 Co-regulates EP300–BRG1-dependent transcription of genes involved in breast cancer cell proliferation and DNA repair

Sobczak, M., Pitt, A. R., Spickett, C. M. & Robaszkiewicz, A., 11 Oct 2019, In : Cancers. 11, 10, 1539.

Research output: Contribution to journalArticle

Open Access
File

Redox lipidomics and adductomics - Advanced analytical strategies to study oxidized lipids and lipid-protein adducts

Cruciani, G., Domingues, P., Fedorova, M., Galli, F. & Spickett, C. M., 1 Nov 2019, In : Free Radical Biology and Medicine. 144, p. 1-5

Research output: Contribution to journalEditorial

Short-chain lipid peroxidation products form covalent adducts with pyruvate kinase and inhibit its activity in vitro and in breast cancer cells

Costa Sousa, B., Ahmed, T., Dann, W. L., Ashman, J., Guy, A., Durand, T., Pitt, A. R. & Spickett, C. M., 20 Nov 2019, In : Free Radical Biology and Medicine. 144, p. 223-233 11 p.

Research output: Contribution to journalArticle

Open Access
File

SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis

Pollock, N. L., Rai, M., Simon, K. S., Hesketh, S. J., Teo, A. C. K., Parmar, M., Sridhar, P., Collins, R., Lee, S. C., Stroud, Z. N., Bakker, S. E., Muench, S. P., Barton, C. H., Hurlbut, G., Roper, D. I., Smith, C. J. I., Knowles, T. J., Spickett, C. M., East, J. M., Postis, V. & 1 others, Dafforn, T. R., 1 Aug 2019, In : BBA -Biomembranes. 1861, 8, p. 1437-1445 9 p.

Research output: Contribution to journalArticle

Open Access
File

The interaction of silver(II) complexes with biological macromolecules and antioxidants

Trotter, K. D., Owojaiye, O., Meredith, S., Keating, P. E., Spicer, M. D., Reglinski, J. & Spickett, C. M., 1 Aug 2019, In : BioMetals. 32, 4, p. 627-640 14 p.

Research output: Contribution to journalArticle

Open Access
File
2018

A high-sensitivity electrochemiluminescence-based ELISA for the measurement of the oxidative stress biomarker, 3-nitrotyrosine, in human blood serum and cells

Knight, A. R., Taylor, E., Lukaszewski, R., Tveen Jensen, K., Jones, H. E., Carré, J. E., supov, M. N. I., Littlechild, J. A., Bailey, S. J., Brewer, E., McDonald, T. J., Pitt, A. R., Spickett, C. M. & Winyard, P. G., 20 May 2018, In : Free Radical Biology and Medicine. 120, p. 246-254

Research output: Contribution to journalArticle

Open Access
File

A mass spectrometry approach for the identification and localization of small aldehyde modifications of proteins

Afonso, C. B., Sousa, B. C., Pitt, A. R. & Spickett, C. M., 15 May 2018, In : Archives of Biochemistry and Biophysics. 646, p. 38-45

Research output: Contribution to journalArticle

Open Access
File

Analysis of phospholipid peroxidation and protein lipoxidation products by LC-MS

Spickett, C. M., Sousa, B. C. & Pitt, A. R., 15 Aug 2018, In : Free Radical Biology and Medicine. 124, p. 560

Research output: Contribution to journalConference abstract

Detecting lipoxidation: diagnostic tools for the translation and commercial application

Campos-Pinto, I., Wilkins, J., Schouten, J., Davis, P. & Spickett, C. M., 15 Aug 2018, In : Free Radical Biology and Medicine. 124, p. 566

Research output: Contribution to journalConference abstract

Developing targeted mass spectrometry approaches for the identification of protein lipoxidation

Spickett, C. M., Bhurrut, E. J. B., Sousa, B. C., Afonso, C. B., Durand, T. & Pitt, A. R., 14 May 2018, In : Free Radical Biology and Medicine. 120, Suppl 1, p. S86-S87

Research output: Contribution to journalConference abstract

Effect of short-chain aldehydes on the enzymatic activity of pyruvate kinase

Sousa, B. C., Ashman, J., Spickett, C. M. & Pitt, A. R., 14 May 2018, In : Free Radical Biology and Medicine. 120, Suppl 1, p. S32

Research output: Contribution to journalConference abstract

Immunodetecting lipoxidation: epitope mapping of HNE-modified human serum albumin using two different anti-HNE pAbs

Campos-pinto, I., Wilkins, J., Schouten, J., Pitt, A., Spickett, C. & Davis, P., 20 May 2018, In : Free Radical Biology and Medicine. 120, Suppl 1, p. S50-S51

Research output: Contribution to journalConference abstract

PARP1 promoter links cell cycle progression with adaptation to oxidative environment

Pietrzak, J., Spickett, C. M., Płoszajc, T., Virág, L. & Robaszkiewicz, A., 2 Jun 2018, In : Redox biology. 18, p. 1-5

Research output: Contribution to journalReview article

Open Access
File

Reprint of: Proteomics in cardiovascular diseases: Unveiling sex and gender differences in the era of precision medicine

Baetta, R., Pontremoli, M., Fernandez, A. M., Spickett, C. M. & Banfi, C., 30 Apr 2018, In : Journal of Proteomics. 178, p. 57-72

Research output: Contribution to journalArticle

Open Access
File

The effect of HOCl-induced modifications on phosphatase and tensin homolog (PTEN) structure and function

Verrastro, I., Tveen Jensen, K., Spickett, C. M. & Pitt, A. R., 3 Jan 2018, In : Free Radical Research. p. 1-471

Research output: Contribution to journalArticle

Open Access
File

YO-1 Synthesis and separation of peptide-oxidized phospholipid adducts, a potential lipoxidation marker

Afonso, C. B., Pitt, A. R. & Spickett, C. M., 14 May 2018, In : Free Radical Biology and Medicine. 120, Suppl 1, p. S27

Research output: Contribution to journalConference abstract

2017

Anti-myeloperoxidase antibodies attenuate the monocyte response to LPS and shape macrophage development

Popat, R. J., Hakki, S., Thakker, A., Coughlan, A. M., Watson, J., Little, M. A., Spickett, C. M., Lavender, P., Afzali, B., Kemper, C. & Robson, M. G., 26 Jan 2017, In : JCI Insight. 2, 2, e87379.

Research output: Contribution to journalArticle

Open Access
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Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds

Sousa, B. C., Pitt, A. R. & Spickett, C. M., 10 Feb 2017, In : Free Radical Biology and Medicine. In press

Research output: Contribution to journalArticle

Open Access
File

Corrigendum to "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)" [Redox Biol. 13 (2017) 94-162]

Egea, J., Fabregat, I., Frapart, Y. M., Ghezzi, P., Görlach, A., Kietzmann, T., Kubaichuk, K., Knaus, U. G., Lopez, M. G., Olaso-Gonzalez, G., Petry, A., Schulz, R., Vina, J., Winyard, P., Abbas, K., Ademowo, O. S., Afonso, C. B., Andreadou, I., Antelmann, H., Antunes, F. & 131 others, Aslan, M., Bachschmid, M. M., Barbosa, R. M., Belousov, V., Berndt, C., Bernlohr, D., Bertrán, E., Bindoli, A., Bottari, S. P., Brito, P. M., Carrara, G., Casas, A. I., Chatzi, A., Chondrogianni, N., Conrad, M., Cooke, M. S., Costa, J. G., Cuadrado, A., My-Chan Dang, P., De Smet, B., Debelec-Butuner, B., Dias, I. H. K., Dunn, J. D., Edson, A. J., El Assar, M., El-Benna, J., Ferdinandy, P., Fernandes, A. S., Fladmark, K. E., Förstermann, U., Giniatullin, R., Giricz, Z., Görbe, A., Griffiths, H., Hampl, V., Hanf, A., Herget, J., Hernansanz-Agustín, P., Hillion, M., Huang, J., Ilikay, S., Jansen-Dürr, P., Jaquet, V., Joles, J. A., Kalyanaraman, B., Kaminskyy, D., Karbaschi, M., Kleanthous, M., Klotz, L. O., Korac, B., Korkmaz, K. S., Koziel, R., Kračun, D., Krause, K. H., Křen, V., Krieg, T., Laranjinha, J., Lazou, A., Li, H., Martínez-Ruiz, A., Matsui, R., McBean, G. J., Meredith, S. P., Messens, J., Miguel, V., Mikhed, Y., Milisav, I., Milković, L., Miranda-Vizuete, A., Mojović, M., Monsalve, M., Mouthuy, P. A., Mulvey, J., Münzel, T., Muzykantov, V., Nguyen, I. T. N., Oelze, M., Oliveira, N. G., Palmeira, C. M., Papaevgeniou, N., Pavićević, A., Pedre, B., Peyrot, F., Phylactides, M., Pircalabioru, G. G., Pitt, A. R., Poulsen, H. E., Prieto, I., Rigobello, M. P., Robledinos-Antón, N., Rodríguez-Mañas, L., Rolo, A. P., Rousset, F., Ruskovska, T., Saraiva, N., Sasson, S., Schröder, K., Semen, K., Seredenina, T., Shakirzyanova, A., Smith, G. L., Soldati, T., Sousa, B. C., Spickett, C. M., Stancic, A., Stasia, M. J., Steinbrenner, H., Stepanić, V., Steven, S., Tokatlidis, K., Tuncay, E., Turan, B., Ursini, F., Vacek, J., Vajnerova, O., Valentová, K., Van Breusegem, F., Varisli, L., Veal, E. A., Yalçin, A. S., Yelisyeyeva, O., Žarković, N., Zatloukalová, M., Zielonka, J., Touyz, R. M., Papapetropoulos, A., Grune, T., Lamas, S., Schmidt, H. H. H. W., Di Lisa, F. & Daiber, A., 26 Oct 2017, In : Redox Biology. In press

Research output: Contribution to journalArticle

Open Access
File

European contribution to the study of ROS: a summary of the findings and prospects for the future from the COST Action BM1203 (EU-ROS)

Egea, J., Fabregat, I., Frapart, Y. M., Ghezzi, P., Görlach, A., Kietzmann, T., Kubaichuk, K., Knaus, U. G., Lopez, M. G., Olaso-Gonzalez, G., Petry, A., Schulz, R., Vina, J., Winyard, P., Abbas, K., Ademowo, S., Afonso, C. B., Andreadou, I., Antelmann, H., Antunes, F. & 131 others, Aslan, M., Bachschmid, M. M., Barbosa, R. M., Belousov, V., Berndt, C., Bernlohr, D., Bertrán, E., Bindoli, A., Bottari, S. P., Brito, P. M., Carrara, G., Casas, A. I., Chatzi, A., Chondrogianni, N., Conrad, M., Cooke, M. S., Costa, J. G., Cuadrado, A., My-Chan Dang, P., De Smet, B., Debelec–butuner, B., Dias, I., Dunn, J. D., Edson, A. J., El Assar, M., El-Benna, J., Ferdinandy, P., Fernandes, A. S., Fladmark, K. E., Förstermann, U., Giniatullin, R., Giricz, Z., Görbe, A., Griffiths, H., Hampl, V., Hanf, A., Herget, J., Hernansanz-Agustín, P., Hillion, M., Huang, J., Ilikay, S., Jansen-Dürr, P., Jaquet, V., Joles, J. A., Kalyanaraman, B., Kaminskyy, D., Karbaschi, M., Kleanthous, M., Klotz, L-O., Korac, B., Korkmaz, K. S., Koziel, R., Kračun, D., Krause, K-H., Křen, V., Krieg, T., Laranjinha, J., Lazou, A., Li, H., Martínez-Ruiz, A., Matsui, R., Mcbean, G. J., Meredith, S. P., Messens, J., Miguel, V., Mikhed, Y., Milisav, I., Milković, L., Miranda-Vizuete, A., Mojović, M., Monsalve, M., Mouthuy, P-A., Mulvey, J., Münzel, T., Muzykantov, V., Nguyen, I. T. N., Oelze, M., Oliveira, N. G., Palmeira, C. M., Papaevgeniou, N., Pavićević, A., Pedre, B., Peyrot, F., Phylactides, M., Pircalabioru, G. G., Pitt, A. R., Poulsen, H. E., Prieto, I., Rigobello, M. P., Robledinos-Antón, N., Rodríguez-mañas, L., Rolo, A. P., Rousset, F., Ruskovska, T., Saraiva, N., Sasson, S., Schröder, K., Semen, K., Seredenina, T., Shakirzyanova, A., Smith, G. L., Soldati, T., Sousa, B. C., Spickett, C. M., Stancic, A., Stasia, M. J., Steinbrenner, H., Stepanić, V., Steven, S., Tokatlidis, K., Tuncay, E., Turan, B., Ursini, F., Vacek, J., Vajnerova, O., Valentová, K., van Breusegem, F., Varisli, L., Veal, E. A., Yalçın, A. S., Yelisyeyeva, O., Žarković, N., Zatloukalová, M., Zielonka, J., Touyz, R. M., Papapetropoulos, A., Grune, T., Lamas, S., Schmidt, H. H. H. W., di Lisa, F. & Daiber, A., 1 Oct 2017, In : Redox biology. 13, p. 94-162 69 p.

Research output: Contribution to journalArticle

Open Access
File

Proteomics in cardiovascular diseases: Unveiling sex and gender differences in the era of precision medicine

Baetta, R., Pontremoli, M., Fernandez, A. M., Spickett, C. M. & Banfi, C., 24 Nov 2017, In : Journal of Proteomics. Early online

Research output: Contribution to journalArticle

Open Access
File
2016

Generation of antibodies with varying sequence specifity for Oxidatively-modified peptides from human fibrinogen

Meredith, S. P., Parekh, G., Schouten, J., Wilkins, J., Griffiths, H. R., Davis, P. & Spickett, C. M., 1 Jul 2016, p. S28.

Research output: Contribution to conferenceAbstract

Mass spectrometry based method for measurement of oxysterols

Dias, I. H. K., Milic, I., Devitt, A., Spickett, C. M., Pitt, A. R. & Griffiths, H. R., 1 Jul 2016, p. S27.

Research output: Contribution to conferenceAbstract

Reversible oxidation of phosphatase and tensin homolog (PTEN) alters its interactions with signaling and regulatory proteins

Verrastro, I., Tveen-Jensen, K., Woscholski, R., Spickett, C. M. & Pitt, A. R., 1 Jan 2016, In : Free Radical Biology and Medicine. 90, p. 24-34 11 p.

Research output: Contribution to journalArticle

Open Access
File

Testing anti-sera raised against synthetic fibrinogen peptides with oxidative modifications

Ali, A. Y., Meredith, S. P. & Spickett, C. M., 1 Jul 2016, p. S47.

Research output: Contribution to conferenceAbstract

The effect of cellular oxidative stress on PTEN interactions

Kativu, P., Pitt, A. R. & Spickett, C. M., 1 Jul 2016, p. S57-S58.

Research output: Contribution to conferenceAbstract

2015

Copper complexes as a source of redox active MRI contrast agents

Dunbar, L., Sowden, R. J., Trotter, K. D., Taylor, M. K., Smith, D., Kennedy, A. R., Reglinski, J. & Spickett, C. M., 2015, In : BioMetals. 28, 5, p. 903-912 10 p.

Research output: Contribution to journalArticle

Open Access
File

Differential effects of chlorinated and oxidized phospholipids in vascular tissue: implications for neointima formation

Greig, F. H., Hutchison, L., Spickett, C. M. & Kennedy, S., 1 May 2015, In : Clinical Science. 128, 9, p. 579-592 14 p.

Research output: Contribution to journalArticle

Open Access
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Exploring oxidative modifications of tyrosine: an update on mechanisms of formation, advances in analysis and biological consequences

Houée-Lévin, C., Bobrowski, K., Horakova, L., Karademir, B., Schöneich, C., Davies, M. J. & Spickett, C. M., 2015, In : Free Radical Research. 49, 4, p. 347-373 27 p.

Research output: Contribution to journalArticle

Open Access
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Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

Tveen Jensen, K., Gesellchen, F., Wilson, R., Spickett, C., Cooper, J. M. & Pitt, A., 15 May 2015, In : Scientific Reports. 5, 5 p., 9736.

Research output: Contribution to journalArticle

Open Access
File

Investigating the ability of antibodies to recognize specific oxidized protein epitopes

Meredith, S., Spickett, C., Parekh, G., Schouten, J., Griffiths, H. & Davis, P., Sep 2015, In : Free Radical Biology and Medicine. 86, Suppl.1, p. S31 1 p., PP36.

Research output: Contribution to journalConference abstract

Lipid oxidation

Fauzi, N. M. & Spickett, C. M., 14 Oct 2015, Studies on experimental toxicology and pharmacology. Roberts, S. M., Kehrer, J. P. & Klotz, L-O. (eds.). Cham (CH): Springer, p. 43-79 37 p. (Oxidative stress in applied basic research and clinical practice).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Mass spectrometry-based methods for identifying oxidized proteins in disease: advances and challenges

Verrastro, I., Pasha, S., Jensen, K. T., Pitt, A. R. & Spickett, C. M., 14 Apr 2015, In : Biomolecules. 5, 2, p. 378-411 34 p.

Research output: Contribution to journalReview article

Open Access
File

Optimisation of chromatographic separation of oxidised phospholipids and detection with targeted approaches provides greater coverage of the oxidised lipidome

Thakker, A., Spickett, C. & Pitt, A., Sep 2015, In : Free Radical Biology and Medicine. 86, Suppl.1, p. S25 1 p., PP18.

Research output: Contribution to journalConference abstract

Oxidiative lipidomics coming of age: advances in analysis of oxidized phospholipids in physiology and pathology

Spickett, C. M. & Pitt, A. R., 20 Jun 2015, In : Antioxidants and Redox Signaling. 22, 18, p. 1646-1666 21 p.

Research output: Contribution to journalArticle

Open Access
File

Protein lipoxidation: detection strategies and challenges

Aldini, G., Domingues, M. R., Spickett, C. M., Domingues, P., Altomare, A., Sánchez-Gómez, F. J., Oeste, C. L. & Pérez-Sala, D., Aug 2015, In : Redox biology. 5, p. 253-266 14 p.

Research output: Contribution to journalArticle

Open Access
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Special issue on "analytical methods for the detection of oxidized biomolecules and antioxidants"

Parsons, B. J. & Spickett, C. M., May 2015, In : Free Radical Research. 49, 5, p. 473-476 4 p.

Research output: Contribution to journalEditorial

Open Access
File

The hypotensive effect of acute and chronic AMP-activated protein kinase activation in normal and hyperlipidemic mice

Greig, F. H., Ewart, M-A., McNaughton, E., Cooney, J., Spickett, C. M. & Kennedy, S., Nov 2015, In : Vascular Pharmacology. 74, p. 93-102 10 p.

Research output: Contribution to journalArticle

Open Access
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Top-down lipidomics of low density lipoprotein reveal altered lipid profiles in advanced chronic kidney disease

Reis, A., Rudnitskaya, A., Chariyavilaskul, P., Dhaun, N., Melville, V., Goddard, J., Webb, D. J., Pitt, A. R. & Spickett, C. M., 1 Feb 2015, In : Journal of Lipid Research. 56, 2, p. 413-422 10 p.

Research output: Contribution to journalArticle

Open Access
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Uncovering changes in the redox protein interactome of PTEN

Spickett, C. M., Verrastro, I. & Pitt, A. R., Sep 2015, In : Free Radical Biology and Medicine. 86, Suppl.1, p. S9 1 p., OP15.

Research output: Contribution to journalConference abstract

2014

Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study

Pasha, S., Tveen Jensen, K., Pitt, A. R. & Spickett, C. M., Oct 2014, In : Free Radical Biology and Medicine. 75, Suppl.1, p. S44 1 p., P68.

Research output: Contribution to journalConference abstract

Detection of phosphatidylserine with a modified polar head group in human keratinocytes exposed to the radical generator AAPH

Maciel, E., Neves, B. M., Santinha, D., Reis, A., Domingues, P., Cruz, M. T., Pitt, A. R., Spickett, C. M. & Domingues, M. R. M., 15 Apr 2014, In : Archives of Biochemistry and Biophysics. 548, p. 38–45 8 p.

Research output: Contribution to journalArticle

Open Access
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