20112019
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Personal profile

Research Interests

Microbes are used in many industrial processes to produce high-value chemicals. However, these products are often toxic to the cells, limiting the yields obtained. My project aims to identify the changes to microbial membrane protein and lipid compositions which allow them adapt to product toxicity and production stresses; this will ultimately allow us to engineer more tolerant strains to improve the yields and efficiency of these biotechnological processes.

I am also interested in the interactions of a peripheral membrane protein, receptor component protein (RCP), which is thought to modulate signalling of the calcitonin gene-related (CGRP) receptor. This GPCR is implicated in migraine, and a better understanding of its interaction parteners could uncover additional drug targets. I use signalling assays to study this in mammalian cell lines as well as using yeast to overexpress and purify the interacting proteins of interest.  

Fingerprint Dive into the research topics where Sarah Routledge is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 6 Similar Profiles
Recombinant Proteins Medicine & Life Sciences
G-Protein-Coupled Receptors Medicine & Life Sciences
Antifoaming Agents Medicine & Life Sciences
Bioreactors Medicine & Life Sciences
Yeasts Medicine & Life Sciences
Pichia Medicine & Life Sciences
Yeast Chemical Compounds
Membrane Proteins Medicine & Life Sciences

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Research Output 2011 2019

Liposomes as models for membrane integrity

Routledge, S. J., Linney, J. A. & Goddard, A. D., 28 Jun 2019, In : Biochemical Society Transactions. 47, 3, p. 919-932 14 p.

Research output: Contribution to journalReview article

Liposomes
Cell Membrane
Cell membranes
Membranes
Molecules

The structure of the CGRP and related receptors

Simms, J., Routledge, S., Uddin, R. & Poyner, D., 25 May 2018, Handbook of Experimental Pharmacology. Brain, S. & Gepetti, P. (eds.). Springer, p. 23-36 14 p. (Handbook of Experimental Pharmacology; vol. 255).

Research output: Chapter in Book/Report/Conference proceedingChapter

Calcitonin Receptor-Like Protein
Calcitonin Gene-Related Peptide Receptors
Receptor Activity-Modifying Protein 1
Calcitonin Receptors
G-Protein-Coupled Receptors

The effects of RAMPs upon cell signalling

Routledge, S. J., Ladds, G. & Poyner, D. R., 5 Apr 2017, in press

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Proteins
Cell signaling
G-Protein-Coupled Receptors
Signal transduction
Signal Transduction

GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential

Wheatley, M., Charlton, J., Jamshad, M., Routledge, S. J., Bailey, S., La-Borde, P. J., Azam, M. T., Logan, R. T., Bill, R. M., Dafforn, T. R. & Poyner, D. R., 15 Apr 2016, In : Biochemical Society Transactions. 44, 2, p. 619-623 5 p.

Research output: Contribution to journalReview article

Open Access
File
Styrene
G-Protein-Coupled Receptors
Detergents
Lipids
Membranes

The synthesis of recombinant membrane proteins in yeast for structural studies

Routledge, S., Mikaliunaite, L., Patel, A., Clare, M., Cartwright, S. P., Bawa, Z., Wilks, M. D. B., Low, F., Hardy, D., Rothnie, A. J. & Bill, R. M., 15 Feb 2016, In : Methods. 95, p. 26-37 12 p.

Research output: Contribution to journalArticle

Open Access
File
Recombinant Proteins
Yeast
Membrane Proteins
Yeasts
Proteins