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Sarah Routledge


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    Personal profile

    Research Interests

    Microbes are used in many industrial processes to produce high-value chemicals. However, these products are often toxic to the cells, limiting the yields obtained. My project aims to identify the changes to microbial membrane protein and lipid compositions which allow them adapt to product toxicity and production stresses; this will ultimately allow us to engineer more tolerant strains to improve the yields and efficiency of these biotechnological processes.

    I am also interested in the interactions of a peripheral membrane protein, receptor component protein (RCP), which is thought to modulate signalling of the calcitonin gene-related (CGRP) receptor. This GPCR is implicated in migraine, and a better understanding of its interaction parteners could uncover additional drug targets. I use signalling assays to study this in mammalian cell lines as well as using yeast to overexpress and purify the interacting proteins of interest.  

    Fingerprint Dive into the research topics where Sarah Routledge is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

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    Recombinant Proteins Medicine & Life Sciences
    G-Protein-Coupled Receptors Medicine & Life Sciences
    Proteins Medicine & Life Sciences
    Antifoaming Agents Medicine & Life Sciences
    Detergents Medicine & Life Sciences
    Bioreactors Medicine & Life Sciences
    Yeasts Medicine & Life Sciences
    Membrane Proteins Medicine & Life Sciences

    Network Recent external collaboration on country level. Dive into details by clicking on the dots.

    Research Output 2011 2020

    Expression and purification of recombinant G protein-coupled receptors: A review

    Wiseman, D. N., Otchere, A., Patel, J. H., Uddin, R., Pollock, N. L., Routledge, S. J., Rothnie, A. J., Slack, C., Poyner, D. R., Bill, R. M. & Goddard, A. D., 31 Oct 2019, In : Protein Expression and Purification. 167, 105524.

    Research output: Contribution to journalArticle

    Open Access
    Drug Design
    Drug Discovery
    G-Protein-Coupled Receptors
    Recombinant Proteins

    Liposomes as models for membrane integrity

    Routledge, S. J., Linney, J. A. & Goddard, A. D., 28 Jun 2019, In : Biochemical Society Transactions. 47, 3, p. 919-932 14 p.

    Research output: Contribution to journalReview article

    Cell Membrane
    Cell membranes

    Receptor component protein, an endogenous allosteric modulator of family B G protein coupled receptors

    Routledge, S. J., Simms, J., Clark, A., Yeung, H. Y., Wigglesworth, M. J., Dickerson, I. M., Kitchen, P., Ladds, G. & Poyner, D. R., 27 Dec 2019, In : BBA -Biomembranes. 1862, 3, 183174.

    Research output: Contribution to journalArticle

    Open Access
    G-Protein-Coupled Receptors
    Calcitonin Gene-Related Peptide Receptors
    Receptor Activity-Modifying Protein 1

    The structure of the CGRP and related receptors

    Simms, J., Routledge, S., Uddin, R. & Poyner, D., 25 May 2018, Handbook of Experimental Pharmacology. Brain, S. & Gepetti, P. (eds.). Springer, p. 23-36 14 p. (Handbook of Experimental Pharmacology; vol. 255).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Calcitonin Receptor-Like Protein
    Calcitonin Gene-Related Peptide Receptors
    Receptor Activity-Modifying Protein 1
    Calcitonin Receptors
    G-Protein-Coupled Receptors

    The effects of RAMPs upon cell signalling

    Routledge, S. J., Ladds, G. & Poyner, D. R., 5 Apr 2017, In : Molecular and Cellular Endocrinology. in press

    Research output: Contribution to journalArticle

    Open Access
    Receptor Activity-Modifying Proteins
    Cell signaling
    G-Protein-Coupled Receptors
    Signal transduction
    GTP-Binding Proteins