8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus

Joseph Lunec; Karl E. Herbert; Susan Blount; Helen R. Griffiths; Paul Emery

doi:10.1016/0014-5793(94)00583-4

8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus

Joseph Lunec, Karl E. Herbert, Susan Blount, Helen R. Griffiths, Paul Emery

Research output: Contribution to journal › Article › peer-review

Abstract

8-Hydroxydeoxyguanosine (80HDG) is a specific marker of oxidative damage to DNA. We have observed that patients with SLE (systemic lupus erythematosus), have undetectable levels of urinary 80HDG by HPLC. Further analysis by GC-MS confirmed that levels of 80HDG in SLE urine were 10(3)-fold lower than in an age- and sex-matched control group. Experiments utilising cultures of SLE and normal lymphocytes exposed to H2O2 confirmed the impaired ability of SLE lymphocytes to repair 80HDG. We subsequently observed in SLE patients that 80HDG had accumulated in low molecular weight DNA associated with circulating immune complexes. We suggest that oxygen radicals may induce pathology in SLE by maintaining the presence of an antigenic form of DNA in the circulation.

Original language	English
Pages (from-to)	131-138
Number of pages	8
Journal	FEBS Letters
Volume	348
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(94)00583-4
Publication status	Published - 11 Jul 1994

Keywords

systemic lupus erythematosus
rheumatoid arthritis

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10.1016/0014-5793(94)00583-4

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title = "8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus",

abstract = "8-Hydroxydeoxyguanosine (80HDG) is a specific marker of oxidative damage to DNA. We have observed that patients with SLE (systemic lupus erythematosus), have undetectable levels of urinary 80HDG by HPLC. Further analysis by GC-MS confirmed that levels of 80HDG in SLE urine were 10(3)-fold lower than in an age- and sex-matched control group. Experiments utilising cultures of SLE and normal lymphocytes exposed to H2O2 confirmed the impaired ability of SLE lymphocytes to repair 80HDG. We subsequently observed in SLE patients that 80HDG had accumulated in low molecular weight DNA associated with circulating immune complexes. We suggest that oxygen radicals may induce pathology in SLE by maintaining the presence of an antigenic form of DNA in the circulation.",

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T1 - 8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus

AU - Lunec, Joseph

AU - Herbert, Karl E.

AU - Blount, Susan

AU - Griffiths, Helen R.

AU - Emery, Paul

PY - 1994/7/11

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N2 - 8-Hydroxydeoxyguanosine (80HDG) is a specific marker of oxidative damage to DNA. We have observed that patients with SLE (systemic lupus erythematosus), have undetectable levels of urinary 80HDG by HPLC. Further analysis by GC-MS confirmed that levels of 80HDG in SLE urine were 10(3)-fold lower than in an age- and sex-matched control group. Experiments utilising cultures of SLE and normal lymphocytes exposed to H2O2 confirmed the impaired ability of SLE lymphocytes to repair 80HDG. We subsequently observed in SLE patients that 80HDG had accumulated in low molecular weight DNA associated with circulating immune complexes. We suggest that oxygen radicals may induce pathology in SLE by maintaining the presence of an antigenic form of DNA in the circulation.

AB - 8-Hydroxydeoxyguanosine (80HDG) is a specific marker of oxidative damage to DNA. We have observed that patients with SLE (systemic lupus erythematosus), have undetectable levels of urinary 80HDG by HPLC. Further analysis by GC-MS confirmed that levels of 80HDG in SLE urine were 10(3)-fold lower than in an age- and sex-matched control group. Experiments utilising cultures of SLE and normal lymphocytes exposed to H2O2 confirmed the impaired ability of SLE lymphocytes to repair 80HDG. We subsequently observed in SLE patients that 80HDG had accumulated in low molecular weight DNA associated with circulating immune complexes. We suggest that oxygen radicals may induce pathology in SLE by maintaining the presence of an antigenic form of DNA in the circulation.

KW - systemic lupus erythematosus

KW - rheumatoid arthritis

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M3 - Article

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JO - FEBS Letters

JF - FEBS Letters

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ER -

8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus

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