8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus

Joseph Lunec, Karl E. Herbert, Susan Blount, Helen R. Griffiths, Paul Emery

Research output: Contribution to journalArticle

Abstract

8-Hydroxydeoxyguanosine (80HDG) is a specific marker of oxidative damage to DNA. We have observed that patients with SLE (systemic lupus erythematosus), have undetectable levels of urinary 80HDG by HPLC. Further analysis by GC-MS confirmed that levels of 80HDG in SLE urine were 10(3)-fold lower than in an age- and sex-matched control group. Experiments utilising cultures of SLE and normal lymphocytes exposed to H2O2 confirmed the impaired ability of SLE lymphocytes to repair 80HDG. We subsequently observed in SLE patients that 80HDG had accumulated in low molecular weight DNA associated with circulating immune complexes. We suggest that oxygen radicals may induce pathology in SLE by maintaining the presence of an antigenic form of DNA in the circulation.
Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalFEBS Letters
Volume348
Issue number2
DOIs
Publication statusPublished - 11 Jul 1994

Keywords

  • systemic lupus erythematosus
  • rheumatoid arthritis

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    Lunec, J., Herbert, K. E., Blount, S., Griffiths, H. R., & Emery, P. (1994). 8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus. FEBS Letters, 348(2), 131-138. https://doi.org/10.1016/0014-5793(94)00583-4