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2019

Analyzing recombinant protein production in Pichia pastoris with targeted proteomics

Bill, R. M., Ulke-Lemée, A., Cartwright, S. P., Far, R., Kim, J. & MacDonald, J. A., 9 Feb 2019, Recombinant Protein Production in Yeast. Gasser, B. & Mattanovich, D. (eds.). Humana Press, Vol. 1923. p. 187-209 23 p. (Methods in Molecular Biology; vol. 1923).

Research output: Chapter in Book/Report/Conference proceedingChapter

Pichia
Recombinant Proteins
Proteomics
Mass Spectrometry
Antibodies

Functional expression of Multidrug Resistance Protein 4 MRP4/ABCC4

Hardy, D., Bill, R. M., Jawhari, A. & Rothnie, A. J., 1 Dec 2019, In : SLAS Discovery. 24, 10, p. 1000-1008 9 p.

Research output: Contribution to journalArticle

Open Access
File
P-Glycoproteins
Adenosine Triphosphate
Sf9 Cells
Membrane Proteins
Insects

Inhibitors of Mammalian Aquaporin Water Channels

Abir-Awan, M., Kitchen, P., Salman, M. M., Conner, M. T., Conner, A. C. & Bill, R. M., 29 Mar 2019, In : International Journal of Molecular Sciences. 20, 7, 1589.

Research output: Contribution to journalReview article

Open Access
File
Aquaporins
inhibitors
acetazolamide
pathology
metastasis

Lignocellulosic ethanol production: Evaluation of new approaches, cell immobilization and reactor configurations

Karagoz, P., Bill, R. M. & Ozkan, M., 1 Dec 2019, In : Renewable energy. 143, p. 741-752 12 p.

Research output: Contribution to journalReview article

Cell immobilization
Ethanol
Cellulosic ethanol
Biomass
Metabolic engineering

Stabilization of human Multidrug Resistance Protein 4 (MRP4/ABCC4) using novel solubilization agents.

Hardy, D., Bill, R. M., Rothnie, A. J. & Jawhari, A., 1 Dec 2019, In : SLAS Discovery. 24, 10, p. 1009-1017 9 p.

Research output: Contribution to journalArticle

Open Access
File
P-Glycoproteins
Detergents
Calixarenes
Stabilization
Membrane Proteins
2018

Aquaporins and Their Regulation after Spinal Cord Injury

Halsey, A. M., Conner, A. C., Bill, R. M., Logan, A. & Ahmed, Z., 18 Oct 2018, In : Stem cells. 7, 10, 174.

Research output: Contribution to journalReview article

Open Access
File
Aquaporins
Spinal Cord Injuries
Edema
Water Movements
Astrocytes

Biomimetic water channels: general discussion

Baaden, M., Barboiu, M., Bill, R. M., Chen, C-L., Davis, J., Di Vincenzo, M., Freger, V., Fröba, M., Gale, P. A., Gong, B., Hélix-Nielsen, C., Hickey, R., Hinds, B., Hou, J-L., Hummer, G., Kumar, M., Legrand, Y-M., Lokesh, M., Mi, B., Murail, S. & 7 others, Pohl, P., Sansom, M., Song, Q., Song, W., Törnroth-Horsefield, S., Vashisth, H. & Vögele, M., 18 Sep 2018, In : Faraday Discussions. 209, 0, p. 205-229 25 p.

Research output: Contribution to journalComment/debate

Downregulation of aquaporin 3 inhibits cellular proliferation, migration and invasion in the MDA‑MB‑231 breast cancer cell line

Arif, M., Kitchen, P., Conner, M., Hill, E., Nagel, D., Bill, R., Dunmore, S., Armesilla, A., Gross, S., Carmichael, A., Conner, A. & Brown, J., May 2018, In : Oncology Letters. 16, 1, p. 713-720

Research output: Contribution to journalArticle

Open Access
File
Aquaporin 3
Aquaporins
Aquaglyceroporins
Glycerol
Gene Knockdown Techniques

Microbial expression systems for membrane proteins

Dilworth, M. V., Piel, M. S., Bettaney, K. E., Ma, P., Luo, J., Sharples, D., Poyner, D. R., Gross, S. R., Moncoq, K., J. F. Henderson, P., Miroux, B. & Bill, R. M., 12 Apr 2018, In : Methods.

Research output: Contribution to journalArticle

Open Access
File
Membrane Proteins
Genetic engineering
Proteins
Recombinant DNA
Crystallization

Recombinant Membrane Protein Methods

Bill, R. M., Goddard, A. D. & Rothnie, A. J., 1 Sep 2018, In : Methods. 147, p. 1-2 2 p.

Research output: Contribution to journalEditorial

Structure and function of natural proteins for water transport: general discussion

Baaden, M., Barboiu, M., Bill, R. M., Casanova, S., Chen, C-L., Conner, M., Freger, V., Gong, B., Góra, A., Hinds, B., Horner, A., Hummer, G., Kumar, M., Lokesh, M., Mitra, S., Noy, A., Pohl, P., Sadet, A., Sansom, M., Törnroth-Horsefield, S. & 1 others, Vashisth, H., 14 Sep 2018, In : Faraday Discussions. 209, 0, p. 83-95 13 p.

Research output: Contribution to journalComment/debate

2017

A new panel of epitope mapped monoclonal antibodies recognising the prototypical tetraspanin CD81

Grove, J., Hu, K., Farquhar, M. J., Goodall, M., Walker, L., Jamshad, M., Drummer, H. E., Bill, R. M., Balfe, P. & McKeating, J. A., 7 Sep 2017, In : Wellcome Open Research. 2, p. 82

Research output: Contribution to journalArticle

Open Access
File
Viruses
Hepacivirus
Epitopes
Tetraspanins
Monoclonal Antibodies

Constitutively-stressed yeast strains are high-yielding for recombinant Fps1: implications for the translational regulation of an aquaporin

Cartwright, S. P., Darby, R. A. J., Sarkar, D., Bonander, N., Gross, S. R., Ashe, M. P. & Bill, R. M., 9 Mar 2017, In : Microbial Cell Factories. 16, 1, 41.

Research output: Contribution to journalArticle

Open Access
File
Aquaporins
Yeast
Yeasts
Open Reading Frames
Doxycycline
Open Access
File
Aquaporin 4
Calcium-Sensing Receptors
Calmodulin
Hypothermia
Astrocytes

Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor

Tran, A-M., Nguyen, T-T., Nguyen, C-T., Huynh-Thi, X-M., Nguyen, C-T., Trinh, M-T., Tran, L-T., Cartwright, S. P., Bill, R. M. & Tran-Van, H., 4 Apr 2017, In : BMC Research Notes. 10, 1, 8 p., 148.

Research output: Contribution to journalArticle

Open Access
File
Pichia
Granulocyte-Macrophage Colony-Stimulating Factor
Yeast
Saccharomyces cerevisiae
Yeasts

Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris

Cartwright, S., Bill, R. M., Bui, S. T., Van, H. T. & Nguyen, H. M., 27 Jul 2017, In : BMC Research Notes. 10, 6 p., 340.

Research output: Contribution to journalArticle

Open Access
File
Hepatitis delta Antigens
Hepatitis Delta Virus
Pichia
Viruses
Yeast

Transcriptome Analysis of Gene Expression Provides New Insights into the Effect of Mild Therapeutic Hypothermia on Primary Human Cortical Astrocytes Cultured under Hypoxia

Salman, M. M., Kitchen, P., Woodroofe, M. N., Bill, R. M., Conner, A. C., Heath, P. R. & Conner, M. T., 14 Dec 2017, In : Frontiers in Cellular Neuroscience. 11, p. 386

Research output: Contribution to journalArticle

Open Access
File
Induced Hypothermia
Gene Expression Profiling
Astrocytes
Gene Expression
Proteins

Transcriptome analysis suggests a role for the differential expression of cerebral aquaporins and the MAPK signalling pathway in human temporal lobe epilepsy

Salman, M. M., Sheilabi, M. A., Bhattacharyya, D., Kitchen, P., Conner, A. C., Bill, R. M., Woodroofe, M. N., Conner, M. T. & Princivalle, A. P., 1 Sep 2017, In : European Journal of Neuroscience. 46, 5, p. 2121-2132 12 p.

Research output: Contribution to journalArticle

Open Access
File
Aquaporins
Temporal Lobe Epilepsy
Gene Expression Profiling
Aquaporin 4
Microarray Analysis
2016

GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential

Wheatley, M., Charlton, J., Jamshad, M., Routledge, S. J., Bailey, S., La-Borde, P. J., Azam, M. T., Logan, R. T., Bill, R. M., Dafforn, T. R. & Poyner, D. R., 15 Apr 2016, In : Biochemical Society Transactions. 44, 2, p. 619-623 5 p.

Research output: Contribution to journalReview article

Open Access
File
Styrene
G-Protein-Coupled Receptors
Detergents
Lipids
Membranes

Membrane protein production in the Yeast, S. cerevisiae

Cartwright, S. P., Mikaliunaite, L. & Bill, R. M., 3 Aug 2016, Heterologous expression of membrane proteins. Mus-Veteau, I. (ed.). New York (US): Springer, p. 23-35 13 p. (Methods in Molecular Biology; vol. 1432).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Saccharomyces cerevisiae
Membrane Proteins
Yeasts
Calcium-Transporting ATPases
Recombinant Proteins

Overcoming bottlenecks in the membrane protein structural biology pipeline

Hardy, D., Bill, R. M., Jawhari, A. & Rothnie, A. J., 9 Jun 2016, In : Biochemical Society Transactions. 44, 3, p. 838-844 7 p.

Research output: Contribution to journalReview article

Open Access
File
Membrane Proteins
Pipelines
Detergents
Protein Stability
Glycols

Structural determinants of oligomerization of the aquaporin-4 channel

Kitchen, P., Conner, M. T., Bill, R. M. & Conner, A. C., 25 Mar 2016, In : Journal of Biological Chemistry. 291, 13, p. 6858-6871 14 p.

Research output: Contribution to journalArticle

Open Access
File
Aquaporin 4
Oligomerization
Aquaporins
Permeability
Water

The synthesis of recombinant membrane proteins in yeast for structural studies

Routledge, S., Mikaliunaite, L., Patel, A., Clare, M., Cartwright, S. P., Bawa, Z., Wilks, M. D. B., Low, F., Hardy, D., Rothnie, A. J. & Bill, R. M., 15 Feb 2016, In : Methods. 95, p. 26-37 12 p.

Research output: Contribution to journalArticle

Open Access
File
Recombinant Proteins
Yeast
Membrane Proteins
Yeasts
Proteins
2015

Beyond water homeostasis: diverse functional roles of mammalian aquaporins

Kitchen, P., Day, R. E., Salman, M. M., Conner, M. T., Bill, R. M. & Conner, A. C., Dec 2015, In : BBA - General Subjects. 1850, 12, p. 2410-2421 12 p.

Research output: Contribution to journalArticle

Open Access
File
Aquaporins
Homeostasis
Water
Cell signaling
Cell Size

Editorial overview: new protein production tools for structural biology

Berger, I. & Bill, R. M., Jun 2015, In : Current Opinion in Structural Biology. 32, p. v–vii 3 p.

Research output: Contribution to journalEditorial

Membrane Proteins
Recombinant Proteins
Proteins
Polyproteins
Magnetic Resonance Spectroscopy

G-protein-coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent

Jamshad, M., Charlton, J., Lin, Y-P., Routledge, S. J., Bawa, Z., Knowles, T. J., Overduin, M., Dekker, N., Dafforn, T. R., Bill, R. M., Poyner, D. R. & Wheatley, M., 27 Feb 2015, In : Bioscience Reports. 35, 2, 10 p., e00188.

Research output: Contribution to journalArticle

Open Access
File
G-Protein-Coupled Receptors
Detergents
Purification
Lipids
Styrene
Open Access
File
Membrane Proteins
Recombinant Proteins
Protein Folding
Plasmids
Mutation

Identification and molecular mechanisms of the rapid tonicity-induced relocalization of the aquaporin 4 channel

Kitchen, P., Day, R. E., Taylor, L. H. J., Salman, M. M., Bill, R. M., Conner, M. T. & Conner, A. C., 3 Jul 2015, In : Journal of Biological Chemistry. 290, p. 16873-16881 9 p.

Research output: Contribution to journalArticle

Open Access
File
Aquaporin 4
Brain Edema
Cyclic AMP-Dependent Protein Kinases
Brain
Phosphorylation

Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level

Prielhofer, R., Cartwright, S., Graf, A. B., Valli, M., Bill, R., Mattanovich, D. & Gasser, B., 11 Mar 2015, In : BMC Genomics. 16, 1, 17 p., 167.

Research output: Contribution to journalArticle

Open Access
File
Pichia
Methanol
Glycerol
Carbon
Glucose

Plasma membrane abundance of human aquaporin 5 is dynamically regulated by multiple pathways

Kitchen, P., Öberg, F., Sjöhamn, J., Hedfalk, K., Bill, R. M., Conner, A. C., Conner, M. T. & Törnroth-Horsefield, S., 16 Nov 2015, In : PLoS ONE. 10, 11, 17 p., e0143027.

Research output: Contribution to journalArticle

Open Access
File
Aquaporin 5
aquaporins
Cell membranes
plasma membrane
Cell Membrane

Rapid tonicity induced re-localisation of endogenous aquaporin 4 in primary rat astrocytes - a therapeutic target for cytotoxic brain oedema?

Day, R., Kitchen, P., Salman, M., Bill, R., Conner, A. & Conner, M., 31 Aug 2015, In : GLIA. 63, Suppl.S1, p. E406-E407 2 p., T13-01B.

Research output: Contribution to journalMeeting abstract

Aquaporin 4
Brain Edema
Astrocytes
Aquaporins
Water

Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?

Bill, R. M., 2015, In : Journal of Pharmacy and Pharmacology. 67, 3, p. 319-328 10 p.

Research output: Contribution to journalArticle

Open Access
File
Subunit Vaccines
Protein Subunits
Recombinant Proteins
Yeasts
Antigens

Structural basis for receptor activity-modifying protein-dependent selective peptide recognition by a G protein-coupled receptor

Booe, JM., Walker, C. S., Barwell, J., Kuteyi, G., Simms, J., Jamaluddin, MA., Warner, ML., Bill, R. M., Harris, PW., Brimble, MA., Poyner, D. R., Hay, D. L. & Pioszak, AA., 18 Jun 2015, In : Molecular Cell. 58, 6, p. 1040-1052 13 p.

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Proteins
Calcitonin Receptor-Like Protein
G-Protein-Coupled Receptors
Peptides
Adrenomedullin