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2019

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

Teo, A. C. K., Lee, S. C., Pollock, N. L., Stroud, Z., Hall, S., Thakker, A., Pitt, A. R., Dafforn, T. R., Spickett, C. M. & Roper, D. I., 12 Feb 2019, In : Scientific Reports. 9, 1, 1813.

Research output: Contribution to journalArticle

Open Access
File
Bacterial Proteins
Phospholipids
Membrane Proteins
Lipids
Styrene

Epitope mapping and characterization of 4-hydroxy-2-nonenal modified-human serum albumin using two different polyclonal antibodies

Campos-pinto, I., Méndez, L., Schouten, J., Wilkins, J., Fedorova, M., Pitt, A., Davis, P. & Spickett, C., 20 Nov 2019, In : Free Radical Biology and Medicine. 144, p. 234-244 11 p.

Research output: Contribution to journalArticle

Open Access
File
Epitope Mapping
Serum Albumin
Epitopes
Antibodies
Peptides

Evaluation of air oxidized PAPC: A multi laboratory study by LC-MS/MS

Ni, Z., Sousa, B. C., Colombo, S., Afonso, C. B., Melo, T., Pitt, A. R., Spickett, C. M., Domingues, P., Domingues, M. R., Fedorova, M. & Criscuolo, A., 20 Nov 2019, In : Free Radical Biology and Medicine. 144, p. 156-166 11 p.

Research output: Contribution to journalArticle

Open Access
File
Air
Lipids
Lipid Peroxidation
Oxidation
Arachidonic Acids

Increased production of 27-hydroxycholesterol in human colorectal cancer advanced stage: Possible contribution to cancer cell survival and infiltration

Rossin, D., Dias, I. H. K., Solej, M., Milic, I., Pitt, A. R., Iaia, N., Scoppapietra, L., Devitt, A., Nano, M., Degiuli, M., Volante, M., Caccia, C., Leoni, V., Griffiths, H. R., Spickett, C. M., Poli, G. & Biasi, F., 20 May 2019, In : Free Radical Biology and Medicine. 136, p. 35-44 10 p.

Research output: Contribution to journalArticle

Infiltration
Colorectal Neoplasms
Cell Survival
Cells
Tumors

PARP1 Co-regulates EP300–BRG1-dependent transcription of genes involved in breast cancer cell proliferation and DNA repair

Sobczak, M., Pitt, A. R., Spickett, C. M. & Robaszkiewicz, A., 11 Oct 2019, In : Cancers. 11, 10, 1539.

Research output: Contribution to journalArticle

Open Access
File
DNA Repair
Cell Proliferation
Breast Neoplasms
Genes
Adenosine Diphosphate

Short-chain lipid peroxidation products form covalent adducts with pyruvate kinase and inhibit its activity in vitro and in breast cancer cells

Costa Sousa, B., Ahmed, T., Dann, W. L., Ashman, J., Guy, A., Durand, T., Pitt, A. R. & Spickett, C. M., 20 Nov 2019, In : Free Radical Biology and Medicine. 144, p. 223-233 11 p.

Research output: Contribution to journalArticle

Open Access
File
Pyruvate Kinase
Aldehydes
Lipid Peroxidation
Cells
Breast Neoplasms
2018

A high-sensitivity electrochemiluminescence-based ELISA for the measurement of the oxidative stress biomarker, 3-nitrotyrosine, in human blood serum and cells

Knight, A. R., Taylor, E., Lukaszewski, R., Tveen Jensen, K., Jones, H. E., Carré, J. E., supov, M. N. I., Littlechild, J. A., Bailey, S. J., Brewer, E., McDonald, T. J., Pitt, A. R., Spickett, C. M. & Winyard, P. G., 20 May 2018, In : Free Radical Biology and Medicine. 120, p. 246-254

Research output: Contribution to journalArticle

Open Access
File
Immunosorbents
Oxidative stress
Biomarkers
Assays
Blood Cells
Open Access
File
Acrolein
Aldehydes
Mass spectrometry
Mass Spectrometry
Muramidase

Analysis of phospholipid peroxidation and protein lipoxidation products by LC-MS

Spickett, C. M., Sousa, B. C. & Pitt, A. R., 15 Aug 2018, In : Free Radical Biology and Medicine. 124, p. 560

Research output: Contribution to journalMeeting abstract

Phospholipids
Proteins
Bioactivity
Mass spectrometry
Apolipoprotein B-100

Developing targeted mass spectrometry approaches for the identification of protein lipoxidation

Spickett, C. M., Bhurrut, E. J. B., Sousa, B. C., Afonso, C. B., Durand, T. & Pitt, A. R., 14 May 2018, In : Free Radical Biology and Medicine. 120, Suppl 1, p. S86-S87

Research output: Contribution to journalMeeting abstract

Effect of short-chain aldehydes on the enzymatic activity of pyruvate kinase

Sousa, B. C., Ashman, J., Spickett, C. M. & Pitt, A. R., 14 May 2018, In : Free Radical Biology and Medicine. 120, Suppl 1, p. S32

Research output: Contribution to journalMeeting abstract

Open Access
File
Hypochlorous Acid
Phosphoric Monoester Hydrolases
Oxidation
Tyrosine
Proteins

YO-1 Synthesis and separation of peptide-oxidized phospholipid adducts, a potential lipoxidation marker

Afonso, C. B., Pitt, A. R. & Spickett, C. M., 14 May 2018, In : Free Radical Biology and Medicine. 120, Suppl 1, p. S27

Research output: Contribution to journalMeeting abstract

2017
Open Access
File
Aldehydes
Lipids
Oxidation
Phospholipids
2-butenal

Corrigendum to "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)" [Redox Biol. 13 (2017) 94-162]

Egea, J., Fabregat, I., Frapart, Y. M., Ghezzi, P., Görlach, A., Kietzmann, T., Kubaichuk, K., Knaus, U. G., Lopez, M. G., Olaso-Gonzalez, G., Petry, A., Schulz, R., Vina, J., Winyard, P., Abbas, K., Ademowo, O. S., Afonso, C. B., Andreadou, I., Antelmann, H., Antunes, F. & 131 others, Aslan, M., Bachschmid, M. M., Barbosa, R. M., Belousov, V., Berndt, C., Bernlohr, D., Bertrán, E., Bindoli, A., Bottari, S. P., Brito, P. M., Carrara, G., Casas, A. I., Chatzi, A., Chondrogianni, N., Conrad, M., Cooke, M. S., Costa, J. G., Cuadrado, A., My-Chan Dang, P., De Smet, B., Debelec-Butuner, B., Dias, I. H. K., Dunn, J. D., Edson, A. J., El Assar, M., El-Benna, J., Ferdinandy, P., Fernandes, A. S., Fladmark, K. E., Förstermann, U., Giniatullin, R., Giricz, Z., Görbe, A., Griffiths, H., Hampl, V., Hanf, A., Herget, J., Hernansanz-Agustín, P., Hillion, M., Huang, J., Ilikay, S., Jansen-Dürr, P., Jaquet, V., Joles, J. A., Kalyanaraman, B., Kaminskyy, D., Karbaschi, M., Kleanthous, M., Klotz, L. O., Korac, B., Korkmaz, K. S., Koziel, R., Kračun, D., Krause, K. H., Křen, V., Krieg, T., Laranjinha, J., Lazou, A., Li, H., Martínez-Ruiz, A., Matsui, R., McBean, G. J., Meredith, S. P., Messens, J., Miguel, V., Mikhed, Y., Milisav, I., Milković, L., Miranda-Vizuete, A., Mojović, M., Monsalve, M., Mouthuy, P. A., Mulvey, J., Münzel, T., Muzykantov, V., Nguyen, I. T. N., Oelze, M., Oliveira, N. G., Palmeira, C. M., Papaevgeniou, N., Pavićević, A., Pedre, B., Peyrot, F., Phylactides, M., Pircalabioru, G. G., Pitt, A. R., Poulsen, H. E., Prieto, I., Rigobello, M. P., Robledinos-Antón, N., Rodríguez-Mañas, L., Rolo, A. P., Rousset, F., Ruskovska, T., Saraiva, N., Sasson, S., Schröder, K., Semen, K., Seredenina, T., Shakirzyanova, A., Smith, G. L., Soldati, T., Sousa, B. C., Spickett, C. M., Stancic, A., Stasia, M. J., Steinbrenner, H., Stepanić, V., Steven, S., Tokatlidis, K., Tuncay, E., Turan, B., Ursini, F., Vacek, J., Vajnerova, O., Valentová, K., Van Breusegem, F., Varisli, L., Veal, E. A., Yalçin, A. S., Yelisyeyeva, O., Žarković, N., Zatloukalová, M., Zielonka, J., Touyz, R. M., Papapetropoulos, A., Grune, T., Lamas, S., Schmidt, H. H. H. W., Di Lisa, F. & Daiber, A., 26 Oct 2017, In : Redox Biology. In press

Research output: Contribution to journalArticle

Open Access
File

European contribution to the study of ROS: a summary of the findings and prospects for the future from the COST Action BM1203 (EU-ROS)

Egea, J., Fabregat, I., Frapart, Y. M., Ghezzi, P., Görlach, A., Kietzmann, T., Kubaichuk, K., Knaus, U. G., Lopez, M. G., Olaso-Gonzalez, G., Petry, A., Schulz, R., Vina, J., Winyard, P., Abbas, K., Ademowo, S., Afonso, C. B., Andreadou, I., Antelmann, H., Antunes, F. & 131 others, Aslan, M., Bachschmid, M. M., Barbosa, R. M., Belousov, V., Berndt, C., Bernlohr, D., Bertrán, E., Bindoli, A., Bottari, S. P., Brito, P. M., Carrara, G., Casas, A. I., Chatzi, A., Chondrogianni, N., Conrad, M., Cooke, M. S., Costa, J. G., Cuadrado, A., My-Chan Dang, P., De Smet, B., Debelec–butuner, B., Dias, I., Dunn, J. D., Edson, A. J., El Assar, M., El-Benna, J., Ferdinandy, P., Fernandes, A. S., Fladmark, K. E., Förstermann, U., Giniatullin, R., Giricz, Z., Görbe, A., Griffiths, H., Hampl, V., Hanf, A., Herget, J., Hernansanz-Agustín, P., Hillion, M., Huang, J., Ilikay, S., Jansen-Dürr, P., Jaquet, V., Joles, J. A., Kalyanaraman, B., Kaminskyy, D., Karbaschi, M., Kleanthous, M., Klotz, L-O., Korac, B., Korkmaz, K. S., Koziel, R., Kračun, D., Krause, K-H., Křen, V., Krieg, T., Laranjinha, J., Lazou, A., Li, H., Martínez-Ruiz, A., Matsui, R., Mcbean, G. J., Meredith, S. P., Messens, J., Miguel, V., Mikhed, Y., Milisav, I., Milković, L., Miranda-Vizuete, A., Mojović, M., Monsalve, M., Mouthuy, P-A., Mulvey, J., Münzel, T., Muzykantov, V., Nguyen, I. T. N., Oelze, M., Oliveira, N. G., Palmeira, C. M., Papaevgeniou, N., Pavićević, A., Pedre, B., Peyrot, F., Phylactides, M., Pircalabioru, G. G., Pitt, A. R., Poulsen, H. E., Prieto, I., Rigobello, M. P., Robledinos-Antón, N., Rodríguez-mañas, L., Rolo, A. P., Rousset, F., Ruskovska, T., Saraiva, N., Sasson, S., Schröder, K., Semen, K., Seredenina, T., Shakirzyanova, A., Smith, G. L., Soldati, T., Sousa, B. C., Spickett, C. M., Stancic, A., Stasia, M. J., Steinbrenner, H., Stepanić, V., Steven, S., Tokatlidis, K., Tuncay, E., Turan, B., Ursini, F., Vacek, J., Vajnerova, O., Valentová, K., van Breusegem, F., Varisli, L., Veal, E. A., Yalçın, A. S., Yelisyeyeva, O., Žarković, N., Zatloukalová, M., Zielonka, J., Touyz, R. M., Papapetropoulos, A., Grune, T., Lamas, S., Schmidt, H. H. H. W., di Lisa, F. & Daiber, A., 1 Oct 2017, In : Redox biology. 13, p. 94-162 69 p.

Research output: Contribution to journalArticle

Open Access
File
Reactive Nitrogen Species
Oxidation-Reduction
Oxidative stress
Technology
Reactive Oxygen Species
2016

Mass spectrometry based method for measurement of oxysterols

Dias, I. H. K., Milic, I., Devitt, A., Spickett, C. M., Pitt, A. R. & Griffiths, H. R., 1 Jul 2016, p. S27.

Research output: Contribution to conferenceAbstract

Open Access
File
Phosphoric Monoester Hydrolases
Oxidation
Oxidation-Reduction
Proteins
Dual-Specificity Phosphatases

The effect of cellular oxidative stress on PTEN interactions

Kativu, P., Pitt, A. R. & Spickett, C. M., 1 Jul 2016, p. S57-S58.

Research output: Contribution to conferenceAbstract

Phosphoric Monoester Hydrolases
Oxidation-Reduction
Oxidative Stress
Thioredoxins
Phosphatidylinositol 3-Kinases
2015

Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

Tveen Jensen, K., Gesellchen, F., Wilson, R., Spickett, C., Cooper, J. M. & Pitt, A., 15 May 2015, In : Scientific Reports. 5, 5 p., 9736.

Research output: Contribution to journalArticle

Open Access
File
Liquid chromatography
Surface waves
Mass spectrometry
Mass spectrometers
Acoustic waves

Mass spectrometry-based methods for identifying oxidized proteins in disease: advances and challenges

Verrastro, I., Pasha, S., Jensen, K. T., Pitt, A. R. & Spickett, C. M., 14 Apr 2015, In : Biomolecules. 5, 2, p. 378-411 34 p.

Research output: Contribution to journalReview article

Open Access
File
Mass spectrometry
Mass Spectrometry
Post Translational Protein Processing
Proteins
Oxidation
Mass spectrometry
Mass Spectrometry
Phospholipids
divinyl benzene
Phosphatidylcholines
Open Access
File
Physiology
Pathology
Mass spectrometry
Mass Spectrometry
Phospholipids

Top-down lipidomics of low density lipoprotein reveal altered lipid profiles in advanced chronic kidney disease

Reis, A., Rudnitskaya, A., Chariyavilaskul, P., Dhaun, N., Melville, V., Goddard, J., Webb, D. J., Pitt, A. R. & Spickett, C. M., 1 Feb 2015, In : Journal of Lipid Research. 56, 2, p. 413-422 10 p.

Research output: Contribution to journalArticle

Open Access
File
Chronic Renal Insufficiency
LDL Lipoproteins
Lipids
Ethanolamines
Sulfoglycosphingolipids

Uncovering changes in the redox protein interactome of PTEN

Spickett, C. M., Verrastro, I. & Pitt, A. R., Sep 2015, In : Free Radical Biology and Medicine. 86, Suppl.1, p. S9 1 p., OP15.

Research output: Contribution to journalMeeting abstract

Phosphoprotein Phosphatases
Phosphoric Monoester Hydrolases
Oxidation-Reduction
Tensins
Proteins