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• Abstract

  Mass spectrometry based method for measurement of oxysterols

  Dias, I. H. K., Milic, I., Devitt, A., Spickett, C. M., Pitt, A. R. & Griffiths, H. R., 1 Jul 2016, p. S27.

  Research output: Contribution to conference › Abstract › peer-review

• The effect of cellular oxidative stress on PTEN interactions

  Kativu, P., Pitt, A. R. & Spickett, C. M., 1 Jul 2016, p. S57-S58.

  Research output: Contribution to conference › Abstract › peer-review

  • Tensins
  • Phosphoric Monoester Hydrolases
  • Oxidation-Reduction
  • Oxidative Stress
  • Thioredoxins
• Article

  A high-sensitivity electrochemiluminescence-based ELISA for the measurement of the oxidative stress biomarker, 3-nitrotyrosine, in human blood serum and cells

  Knight, A. R., Taylor, E., Lukaszewski, R., Tveen Jensen, K., Jones, H. E., Carré, J. E., supov, M. N. I., Littlechild, J. A., Bailey, S. J., Brewer, E., McDonald, T. J., Pitt, A. R., Spickett, C. M. & Winyard, P. G., 20 May 2018, In: Free Radical Biology and Medicine. 120, p. 246-254

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Oxidative stress
  • Immunosorbents
  • 3-nitrotyrosine
  • Biomarkers
  • Assays

• A mass spectrometry approach for the identification and localization of small aldehyde modifications of proteins

  Afonso, C. B., Sousa, B. C., Pitt, A. R. & Spickett, C. M., 15 May 2018, In: Archives of Biochemistry and Biophysics. 646, p. 38-45

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • pentanal
  • Mass spectrometry
  • Aldehydes
  • Acrolein
  • Mass Spectrometry

• Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

  Teo, A. C. K., Lee, S. C., Pollock, N. L., Stroud, Z., Hall, S., Thakker, A., Pitt, A. R., Dafforn, T. R., Spickett, C. M. & Roper, D. I., 12 Feb 2019, In: Scientific Reports. 9, 1, 1813.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Bacterial Proteins
  • Phospholipids
  • Membrane Proteins
  • maleic acid
  • Styrene

• Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds

  Sousa, B. C., Pitt, A. R. & Spickett, C. M., 10 Feb 2017, In: Free Radical Biology and Medicine. In press

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Aldehydes
  • Oxidation
  • Lipids
  • Phospholipids
  • 4-hydroxy-2-hexenal

• Corrigendum to "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)" [Redox Biol. 13 (2017) 94-162]

  Egea, J., Fabregat, I., Frapart, Y. M., Ghezzi, P., Görlach, A., Kietzmann, T., Kubaichuk, K., Knaus, U. G., Lopez, M. G., Olaso-Gonzalez, G., Petry, A., Schulz, R., Vina, J., Winyard, P., Abbas, K., Ademowo, O. S., Afonso, C. B., Andreadou, I., Antelmann, H., Antunes, F. & 131 others, Aslan, M., Bachschmid, M. M., Barbosa, R. M., Belousov, V., Berndt, C., Bernlohr, D., Bertrán, E., Bindoli, A., Bottari, S. P., Brito, P. M., Carrara, G., Casas, A. I., Chatzi, A., Chondrogianni, N., Conrad, M., Cooke, M. S., Costa, J. G., Cuadrado, A., My-Chan Dang, P., De Smet, B., Debelec-Butuner, B., Dias, I. H. K., Dunn, J. D., Edson, A. J., El Assar, M., El-Benna, J., Ferdinandy, P., Fernandes, A. S., Fladmark, K. E., Förstermann, U., Giniatullin, R., Giricz, Z., Görbe, A., Griffiths, H., Hampl, V., Hanf, A., Herget, J., Hernansanz-Agustín, P., Hillion, M., Huang, J., Ilikay, S., Jansen-Dürr, P., Jaquet, V., Joles, J. A., Kalyanaraman, B., Kaminskyy, D., Karbaschi, M., Kleanthous, M., Klotz, L. O., Korac, B., Korkmaz, K. S., Koziel, R., Kračun, D., Krause, K. H., Křen, V., Krieg, T., Laranjinha, J., Lazou, A., Li, H., Martínez-Ruiz, A., Matsui, R., McBean, G. J., Meredith, S. P., Messens, J., Miguel, V., Mikhed, Y., Milisav, I., Milković, L., Miranda-Vizuete, A., Mojović, M., Monsalve, M., Mouthuy, P. A., Mulvey, J., Münzel, T., Muzykantov, V., Nguyen, I. T. N., Oelze, M., Oliveira, N. G., Palmeira, C. M., Papaevgeniou, N., Pavićević, A., Pedre, B., Peyrot, F., Phylactides, M., Pircalabioru, G. G., Pitt, A. R., Poulsen, H. E., Prieto, I., Rigobello, M. P., Robledinos-Antón, N., Rodríguez-Mañas, L., Rolo, A. P., Rousset, F., Ruskovska, T., Saraiva, N., Sasson, S., Schröder, K., Semen, K., Seredenina, T., Shakirzyanova, A., Smith, G. L., Soldati, T., Sousa, B. C., Spickett, C. M., Stancic, A., Stasia, M. J., Steinbrenner, H., Stepanić, V., Steven, S., Tokatlidis, K., Tuncay, E., Turan, B., Ursini, F., Vacek, J., Vajnerova, O., Valentová, K., Van Breusegem, F., Varisli, L., Veal, E. A., Yalçin, A. S., Yelisyeyeva, O., Žarković, N., Zatloukalová, M., Zielonka, J., Touyz, R. M., Papapetropoulos, A., Grune, T., Lamas, S., Schmidt, H. H. H. W., Di Lisa, F. & Daiber, A., 26 Oct 2017, In: Redox Biology. In press

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Epitope mapping and characterization of 4-hydroxy-2-nonenal modified-human serum albumin using two different polyclonal antibodies

  Campos-pinto, I., Méndez, L., Schouten, J., Wilkins, J., Fedorova, M., Pitt, A., Davis, P. & Spickett, C., 20 Nov 2019, In: Free Radical Biology and Medicine. 144, p. 234-244 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • 2-nonenal
  • Epitope Mapping
  • 4-hydroxy-2-nonenal
  • Human Serum Albumin
  • Epitopes

• European contribution to the study of ROS: a summary of the findings and prospects for the future from the COST Action BM1203 (EU-ROS)

  Egea, J., Fabregat, I., Frapart, Y. M., Ghezzi, P., Görlach, A., Kietzmann, T., Kubaichuk, K., Knaus, U. G., Lopez, M. G., Olaso-Gonzalez, G., Petry, A., Schulz, R., Vina, J., Winyard, P., Abbas, K., Ademowo, S., Afonso, C. B., Andreadou, I., Antelmann, H., Antunes, F. & 131 others, Aslan, M., Bachschmid, M. M., Barbosa, R. M., Belousov, V., Berndt, C., Bernlohr, D., Bertrán, E., Bindoli, A., Bottari, S. P., Brito, P. M., Carrara, G., Casas, A. I., Chatzi, A., Chondrogianni, N., Conrad, M., Cooke, M. S., Costa, J. G., Cuadrado, A., My-Chan Dang, P., De Smet, B., Debelec–butuner, B., Dias, I., Dunn, J. D., Edson, A. J., El Assar, M., El-Benna, J., Ferdinandy, P., Fernandes, A. S., Fladmark, K. E., Förstermann, U., Giniatullin, R., Giricz, Z., Görbe, A., Griffiths, H., Hampl, V., Hanf, A., Herget, J., Hernansanz-Agustín, P., Hillion, M., Huang, J., Ilikay, S., Jansen-Dürr, P., Jaquet, V., Joles, J. A., Kalyanaraman, B., Kaminskyy, D., Karbaschi, M., Kleanthous, M., Klotz, L-O., Korac, B., Korkmaz, K. S., Koziel, R., Kračun, D., Krause, K-H., Křen, V., Krieg, T., Laranjinha, J., Lazou, A., Li, H., Martínez-Ruiz, A., Matsui, R., Mcbean, G. J., Meredith, S. P., Messens, J., Miguel, V., Mikhed, Y., Milisav, I., Milković, L., Miranda-Vizuete, A., Mojović, M., Monsalve, M., Mouthuy, P-A., Mulvey, J., Münzel, T., Muzykantov, V., Nguyen, I. T. N., Oelze, M., Oliveira, N. G., Palmeira, C. M., Papaevgeniou, N., Pavićević, A., Pedre, B., Peyrot, F., Phylactides, M., Pircalabioru, G. G., Pitt, A. R., Poulsen, H. E., Prieto, I., Rigobello, M. P., Robledinos-Antón, N., Rodríguez-mañas, L., Rolo, A. P., Rousset, F., Ruskovska, T., Saraiva, N., Sasson, S., Schröder, K., Semen, K., Seredenina, T., Shakirzyanova, A., Smith, G. L., Soldati, T., Sousa, B. C., Spickett, C. M., Stancic, A., Stasia, M. J., Steinbrenner, H., Stepanić, V., Steven, S., Tokatlidis, K., Tuncay, E., Turan, B., Ursini, F., Vacek, J., Vajnerova, O., Valentová, K., van Breusegem, F., Varisli, L., Veal, E. A., Yalçın, A. S., Yelisyeyeva, O., Žarković, N., Zatloukalová, M., Zielonka, J., Touyz, R. M., Papapetropoulos, A., Grune, T., Lamas, S., Schmidt, H. H. H. W., di Lisa, F. & Daiber, A., 1 Oct 2017, In: Redox biology. 13, p. 94-162 69 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Oxidation-Reduction
  • Oxidative stress
  • Reactive Nitrogen Species
  • Technology
  • Reactive Oxygen Species

• Evaluation of air oxidized PAPC: A multi laboratory study by LC-MS/MS

  Ni, Z., Sousa, B. C., Colombo, S., Afonso, C. B., Melo, T., Pitt, A. R., Spickett, C. M., Domingues, P., Domingues, M. R., Fedorova, M. & Criscuolo, A., 20 Nov 2019, In: Free Radical Biology and Medicine. 144, p. 156-166 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • 2-arachidonoylglycero-3-phosphocholine
  • Air
  • Lipids
  • Lipid Peroxidation
  • Oxidation

• Increased production of 27-hydroxycholesterol in human colorectal cancer advanced stage: Possible contribution to cancer cell survival and infiltration

  Rossin, D., Dias, I. H. K., Solej, M., Milic, I., Pitt, A. R., Iaia, N., Scoppapietra, L., Devitt, A., Nano, M., Degiuli, M., Volante, M., Caccia, C., Leoni, V., Griffiths, H. R., Spickett, C. M., Poli, G. & Biasi, F., 20 May 2019, In: Free Radical Biology and Medicine. 136, p. 35-44 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • 27-hydroxycholesterol
  • Infiltration
  • Oxysterols
  • Cells
  • Colorectal Neoplasms

• Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

  Tveen Jensen, K., Gesellchen, F., Wilson, R., Spickett, C., Cooper, J. M. & Pitt, A., 15 May 2015, In: Scientific Reports. 5, 5 p., 9736.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • liquid chromatography
  • mass spectroscopy
  • ionization
  • mass spectrometers
  • acoustics

• Oxidiative lipidomics coming of age: advances in analysis of oxidized phospholipids in physiology and pathology

  Spickett, C. M. & Pitt, A. R., 20 Jun 2015, In: Antioxidants and Redox Signaling. 22, 18, p. 1646-1666 21 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Mass spectrometry
  • Physiology
  • Pathology
  • Mass Spectrometry
  • Phospholipids

• PARP1 Co-regulates EP300–BRG1-dependent transcription of genes involved in breast cancer cell proliferation and DNA repair

  Sobczak, M., Pitt, A. R., Spickett, C. M. & Robaszkiewicz, A., 11 Oct 2019, In: Cancers. 11, 10, 1539.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Poly (ADP-Ribose) Polymerase-1
  • DNA Repair
  • Cell Proliferation
  • Breast Neoplasms
  • ADP-Ribosylation

• Reversible oxidation of phosphatase and tensin homolog (PTEN) alters its interactions with signaling and regulatory proteins

  Verrastro, I., Tveen-Jensen, K., Woscholski, R., Spickett, C. M. & Pitt, A. R., 1 Jan 2016, In: Free Radical Biology and Medicine. 90, p. 24-34 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Tensins
  • Phosphoric Monoester Hydrolases
  • Oxidation
  • Oxidation-Reduction
  • Proteins

• Short-chain lipid peroxidation products form covalent adducts with pyruvate kinase and inhibit its activity in vitro and in breast cancer cells

  Costa Sousa, B., Ahmed, T., Dann, W. L., Ashman, J., Guy, A., Durand, T., Pitt, A. R. & Spickett, C. M., 20 Nov 2019, In: Free Radical Biology and Medicine. 144, p. 223-233 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Pyruvate Kinase
  • Aldehydes
  • Cells
  • Lipid Peroxidation
  • Acrolein

• The effect of HOCl-induced modifications on phosphatase and tensin homolog (PTEN) structure and function

  Verrastro, I., Tveen Jensen, K., Spickett, C. M. & Pitt, A. R., 3 Jan 2018, In: Free Radical Research. p. 1-471

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Hypochlorous Acid
  • Tensins
  • Phosphoric Monoester Hydrolases
  • Oxidation
  • Tyrosine

• Top-down lipidomics of low density lipoprotein reveal altered lipid profiles in advanced chronic kidney disease

  Reis, A., Rudnitskaya, A., Chariyavilaskul, P., Dhaun, N., Melville, V., Goddard, J., Webb, D. J., Pitt, A. R. & Spickett, C. M., 1 Feb 2015, In: Journal of Lipid Research. 56, 2, p. 413-422 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • LDL Lipoproteins
  • Chronic Renal Insufficiency
  • Lipids
  • oxidized low density lipoprotein
  • Ethanolamines
• Conference abstract

  Analysis of phospholipid peroxidation and protein lipoxidation products by LC-MS

  Spickett, C. M., Sousa, B. C. & Pitt, A. R., 15 Aug 2018, In: Free Radical Biology and Medicine. 124, p. 560

  Research output: Contribution to journal › Conference abstract › peer-review

  • Phospholipids
  • Proteins
  • Bioactivity
  • Mass spectrometry
  • Plasma (human)

• Developing targeted mass spectrometry approaches for the identification of protein lipoxidation

  Spickett, C. M., Bhurrut, E. J. B., Sousa, B. C., Afonso, C. B., Durand, T. & Pitt, A. R., 14 May 2018, In: Free Radical Biology and Medicine. 120, Suppl 1, p. S86-S87

  Research output: Contribution to journal › Conference abstract › peer-review

• Optimisation of chromatographic separation of oxidised phospholipids and detection with targeted approaches provides greater coverage of the oxidised lipidome

  Thakker, A., Spickett, C. & Pitt, A., Sep 2015, In: Free Radical Biology and Medicine. 86, Suppl.1, p. S25 1 p., PP18.

  Research output: Contribution to journal › Conference abstract › peer-review

  • Mass spectrometry
  • Mass Spectrometry
  • Phospholipids
  • Hydrophobic and Hydrophilic Interactions
  • Phosphatidylcholines

• Uncovering changes in the redox protein interactome of PTEN

  Spickett, C. M., Verrastro, I. & Pitt, A. R., Sep 2015, In: Free Radical Biology and Medicine. 86, Suppl.1, p. S9 1 p., OP15.

  Research output: Contribution to journal › Conference abstract › peer-review

  • Oxidation-Reduction
  • Tensins
  • Phosphoprotein Phosphatases
  • Phosphoric Monoester Hydrolases
  • Resins

• YO-1 Synthesis and separation of peptide-oxidized phospholipid adducts, a potential lipoxidation marker

  Afonso, C. B., Pitt, A. R. & Spickett, C. M., 14 May 2018, In: Free Radical Biology and Medicine. 120, Suppl 1, p. S27

  Research output: Contribution to journal › Conference abstract › peer-review
• Review article

  Mass spectrometry-based methods for identifying oxidized proteins in disease: advances and challenges

  Verrastro, I., Pasha, S., Jensen, K. T., Pitt, A. R. & Spickett, C. M., 14 Apr 2015, In: Biomolecules. 5, 2, p. 378-411 34 p.

  Research output: Contribution to journal › Review article › peer-review

  Open Access

  File

  • Mass spectrometry
  • Mass Spectrometry
  • Post Translational Protein Processing
  • Proteins
  • Oxidation