A comparative study of two in-vitro methodologies used to assess E-cigarette cytotoxicity

Pranav Vasanthi Bathri Narayanan, Laura J. Leslie, Lindsay J. Marshall

Research output: Contribution to conferenceAbstract

Abstract

Though widely perceived as harmless, limited information is available on E-cigarette (EC) cytotoxicity. Numerous factors impede EC research, including cell model employed and method of exposure. The present study aims to compare two in vitro methodologies to explore the effects of these choices. Bronchial epithelial cells BEAS-2B were plated in 24 well plates at a density of 3x105 cells/mL/well. Aqueous extracts of a brand anonymised strawberry flavoured EC (ECE) and tobacco cigarette (CSE) were obtained using standard methods and applied to the epithelial cell cultures. Additionally, a multi-cellular human airways model consisting of bronchial epithelial cells and human pulmonary fibroblasts co-cultured at air-liquid interface (ALI) was exposed to the same strawberry flavoured EC vapour (ECV) and whole cigarette smoke (WCS) for 7 mins according to ISO standard using an in-house built smoking machine. 24h post exposure, XTT cell viability analysis showed that, in submerged cultures, ECE and CSE significantly decreased cell viability to 43.60 – 3.65% (p < 0.0001) and 46.27% – 11.37% (p < 0.0001) respectively, compared to untreated cells. In contrast, while exposure of ALI cultures to WCS significantly reduced cell viability to 60.63 – 4.20% (p < 0.0001) compared to cells exposed to air only, ECV exposure had no significant effect on cell viability (103.6 – 15.62% control). These results indicate that the choice of cell model and EC exposure system can significantly impact upon EC cytotoxicity. It also highlights the urgent need for standard testing protocols for EC safety assessment.
Original languageEnglish
Pages244-245
Number of pages2
DOIs
Publication statusPublished - 2016
EventIn Vitro Toxicology Society Annual Meeting - Glasgow, United Kingdom
Duration: 14 Nov 201615 Nov 2016

Meeting

MeetingIn Vitro Toxicology Society Annual Meeting
Abbreviated titleIVTS 2016
CountryUnited Kingdom
CityGlasgow
Period14/11/1615/11/16

Fingerprint

Cell Survival
Tobacco Products
Fragaria
Epithelial Cells
Air
Smoke
In Vitro Techniques
Electronic Cigarettes
Tobacco
Cell Culture Techniques
Fibroblasts
Cell Count
Smoking
Safety
Lung
Research

Bibliographical note

Applied In Vitro Toxicology. December 2016, 2(4): 235-246. doi:10.1089/aivt.2016.29007.abstracts.
Published in Volume: 2 Issue 4: December 1, 2016

Cite this

Vasanthi Bathri Narayanan, P., Leslie, L. J., & Marshall, L. J. (2016). A comparative study of two in-vitro methodologies used to assess E-cigarette cytotoxicity. 244-245. Abstract from In Vitro Toxicology Society Annual Meeting, Glasgow, United Kingdom. https://doi.org/10.1089/aivt.2016.29007.abstracts
Vasanthi Bathri Narayanan, Pranav ; Leslie, Laura J. ; Marshall, Lindsay J. / A comparative study of two in-vitro methodologies used to assess E-cigarette cytotoxicity. Abstract from In Vitro Toxicology Society Annual Meeting, Glasgow, United Kingdom.2 p.
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title = "A comparative study of two in-vitro methodologies used to assess E-cigarette cytotoxicity",
abstract = "Though widely perceived as harmless, limited information is available on E-cigarette (EC) cytotoxicity. Numerous factors impede EC research, including cell model employed and method of exposure. The present study aims to compare two in vitro methodologies to explore the effects of these choices. Bronchial epithelial cells BEAS-2B were plated in 24 well plates at a density of 3x105 cells/mL/well. Aqueous extracts of a brand anonymised strawberry flavoured EC (ECE) and tobacco cigarette (CSE) were obtained using standard methods and applied to the epithelial cell cultures. Additionally, a multi-cellular human airways model consisting of bronchial epithelial cells and human pulmonary fibroblasts co-cultured at air-liquid interface (ALI) was exposed to the same strawberry flavoured EC vapour (ECV) and whole cigarette smoke (WCS) for 7 mins according to ISO standard using an in-house built smoking machine. 24h post exposure, XTT cell viability analysis showed that, in submerged cultures, ECE and CSE significantly decreased cell viability to 43.60 – 3.65{\%} (p < 0.0001) and 46.27{\%} – 11.37{\%} (p < 0.0001) respectively, compared to untreated cells. In contrast, while exposure of ALI cultures to WCS significantly reduced cell viability to 60.63 – 4.20{\%} (p < 0.0001) compared to cells exposed to air only, ECV exposure had no significant effect on cell viability (103.6 – 15.62{\%} control). These results indicate that the choice of cell model and EC exposure system can significantly impact upon EC cytotoxicity. It also highlights the urgent need for standard testing protocols for EC safety assessment.",
author = "{Vasanthi Bathri Narayanan}, Pranav and Leslie, {Laura J.} and Marshall, {Lindsay J.}",
note = "Applied In Vitro Toxicology. December 2016, 2(4): 235-246. doi:10.1089/aivt.2016.29007.abstracts. Published in Volume: 2 Issue 4: December 1, 2016; In Vitro Toxicology Society Annual Meeting, IVTS 2016 ; Conference date: 14-11-2016 Through 15-11-2016",
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Vasanthi Bathri Narayanan, P, Leslie, LJ & Marshall, LJ 2016, 'A comparative study of two in-vitro methodologies used to assess E-cigarette cytotoxicity' In Vitro Toxicology Society Annual Meeting, Glasgow, United Kingdom, 14/11/16 - 15/11/16, pp. 244-245. https://doi.org/10.1089/aivt.2016.29007.abstracts

A comparative study of two in-vitro methodologies used to assess E-cigarette cytotoxicity. / Vasanthi Bathri Narayanan, Pranav; Leslie, Laura J.; Marshall, Lindsay J.

2016. 244-245 Abstract from In Vitro Toxicology Society Annual Meeting, Glasgow, United Kingdom.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - A comparative study of two in-vitro methodologies used to assess E-cigarette cytotoxicity

AU - Vasanthi Bathri Narayanan, Pranav

AU - Leslie, Laura J.

AU - Marshall, Lindsay J.

N1 - Applied In Vitro Toxicology. December 2016, 2(4): 235-246. doi:10.1089/aivt.2016.29007.abstracts. Published in Volume: 2 Issue 4: December 1, 2016

PY - 2016

Y1 - 2016

N2 - Though widely perceived as harmless, limited information is available on E-cigarette (EC) cytotoxicity. Numerous factors impede EC research, including cell model employed and method of exposure. The present study aims to compare two in vitro methodologies to explore the effects of these choices. Bronchial epithelial cells BEAS-2B were plated in 24 well plates at a density of 3x105 cells/mL/well. Aqueous extracts of a brand anonymised strawberry flavoured EC (ECE) and tobacco cigarette (CSE) were obtained using standard methods and applied to the epithelial cell cultures. Additionally, a multi-cellular human airways model consisting of bronchial epithelial cells and human pulmonary fibroblasts co-cultured at air-liquid interface (ALI) was exposed to the same strawberry flavoured EC vapour (ECV) and whole cigarette smoke (WCS) for 7 mins according to ISO standard using an in-house built smoking machine. 24h post exposure, XTT cell viability analysis showed that, in submerged cultures, ECE and CSE significantly decreased cell viability to 43.60 – 3.65% (p < 0.0001) and 46.27% – 11.37% (p < 0.0001) respectively, compared to untreated cells. In contrast, while exposure of ALI cultures to WCS significantly reduced cell viability to 60.63 – 4.20% (p < 0.0001) compared to cells exposed to air only, ECV exposure had no significant effect on cell viability (103.6 – 15.62% control). These results indicate that the choice of cell model and EC exposure system can significantly impact upon EC cytotoxicity. It also highlights the urgent need for standard testing protocols for EC safety assessment.

AB - Though widely perceived as harmless, limited information is available on E-cigarette (EC) cytotoxicity. Numerous factors impede EC research, including cell model employed and method of exposure. The present study aims to compare two in vitro methodologies to explore the effects of these choices. Bronchial epithelial cells BEAS-2B were plated in 24 well plates at a density of 3x105 cells/mL/well. Aqueous extracts of a brand anonymised strawberry flavoured EC (ECE) and tobacco cigarette (CSE) were obtained using standard methods and applied to the epithelial cell cultures. Additionally, a multi-cellular human airways model consisting of bronchial epithelial cells and human pulmonary fibroblasts co-cultured at air-liquid interface (ALI) was exposed to the same strawberry flavoured EC vapour (ECV) and whole cigarette smoke (WCS) for 7 mins according to ISO standard using an in-house built smoking machine. 24h post exposure, XTT cell viability analysis showed that, in submerged cultures, ECE and CSE significantly decreased cell viability to 43.60 – 3.65% (p < 0.0001) and 46.27% – 11.37% (p < 0.0001) respectively, compared to untreated cells. In contrast, while exposure of ALI cultures to WCS significantly reduced cell viability to 60.63 – 4.20% (p < 0.0001) compared to cells exposed to air only, ECV exposure had no significant effect on cell viability (103.6 – 15.62% control). These results indicate that the choice of cell model and EC exposure system can significantly impact upon EC cytotoxicity. It also highlights the urgent need for standard testing protocols for EC safety assessment.

UR - http://online.liebertpub.com/doi/full/10.1089/aivt.2016.29007.abstracts

U2 - 10.1089/aivt.2016.29007.abstracts

DO - 10.1089/aivt.2016.29007.abstracts

M3 - Abstract

SP - 244

EP - 245

ER -

Vasanthi Bathri Narayanan P, Leslie LJ, Marshall LJ. A comparative study of two in-vitro methodologies used to assess E-cigarette cytotoxicity. 2016. Abstract from In Vitro Toxicology Society Annual Meeting, Glasgow, United Kingdom. https://doi.org/10.1089/aivt.2016.29007.abstracts