A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL

Ana Reis, Alisa Rudnitskaya, Gavin J. Blackburn, Norsyahida Mohd Fauzi, Andrew R Pitt, Corinne M Spickett

Research output: Contribution to journalArticle

Abstract

Lipidome profile of fluids and tissues is a growing field as the role of lipids as signaling molecules is increasingly understood, relying on an effective and representative extraction of the lipids present. A number of solvent systems suitable for lipid extraction are commonly in use, though no comprehensive investigation of their effectiveness across multiple lipid classes has been carried out. To address this, human LDL from normolipidemic volunteers was used to evaluate five different solvent extraction protocols [Folch, Bligh and Dyer, acidified Bligh and Dyer, methanol (MeOH)-tert-butyl methyl ether (TBME), and hexane-isopropanol] and the extracted lipids were analyzed by LC-MS in a high-resolution instrument equipped with polarity switching. Overall, more than 350 different lipid species from 19 lipid subclasses were identified. Solvent composition had a small effect on the extraction of predominant lipid classes (triacylglycerides, cholesterol esters, and phosphatidylcholines). In contrast, extraction of less abundant lipids (phosphatidylinositols, lyso-lipids, ceramides, and cholesterol sulfates) was greatly influenced by the solvent system used. Overall, the Folch method was most effective for the extraction of a broad range of lipid classes in LDL, although the hexane-isopropanol method was best for apolar lipids and the MeOH-TBME method was suitable for lactosyl ceramides. Copyright © 2013 by the American Society for Biochemistry and Molecular Biology, Inc.

LanguageEnglish
Pages1812-1824
Number of pages13
JournalJournal of Lipid Research
Volume54
Issue number7
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Solvent extraction
Lipids
2-Propanol
Hexanes
oxidized low density lipoprotein
Lactosylceramides
Cholesterol Esters
Ceramides
Phosphatidylinositols
Phosphatidylcholines
Methanol
Volunteers
Tissue

Keywords

  • anova simultaneous component analysis
  • dual polarity
  • lipidomics
  • liquid-liquid extraction
  • orbitrap
  • polarity switching

Cite this

Reis, Ana ; Rudnitskaya, Alisa ; Blackburn, Gavin J. ; Fauzi, Norsyahida Mohd ; Pitt, Andrew R ; Spickett, Corinne M. / A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL. In: Journal of Lipid Research. 2013 ; Vol. 54, No. 7. pp. 1812-1824.
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A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL. / Reis, Ana; Rudnitskaya, Alisa; Blackburn, Gavin J.; Fauzi, Norsyahida Mohd; Pitt, Andrew R; Spickett, Corinne M.

In: Journal of Lipid Research, Vol. 54, No. 7, 07.2013, p. 1812-1824.

Research output: Contribution to journalArticle

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