A deletion mutation in nfxB of in vitro-induced moxifloxacin-resistant pseudomonas aeruginosa confers multidrug resistance

Van Chi Thai, Hoang V.Y. Pham, Duc Nhat Minh Nguyen, Peter Lambert, Thi Thu Hoai Nguyen*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The modulation of efflux pump functions under fluoroquinolone (FQ) exposure is of great concern as it could result in occurrence of multidrug-resistant (MDR) bacterial strains. In this study, MDR mechanism in Pseudomonas aeruginosa induced via moxifloxacin (MOX) pressure was investigated. After serial MOX [concentration of 0.5 X the minimum inhibitory concentration (MIC)] exposure, the fully susceptible P. aeruginosa ATCC 9027 strain has increased its MIC not only toward MOX (1→128 mg/L) but also to other antibiotics. Furthermore, this MOX-exposed strain did not revert to antibiotic-sensitive phenotype when being cultured in antibiotic-free medium for 12 days. No mutation was observed for FQ-target (gyrA and parC) or most investigated efflux regulatory genes (mexT, mexR, and nalC) except nfxB in which a 100-bp deletion was found. This associated with the elevated expression of multidrug efflux pump operon (mexCD-oprJ) which could directly result in MDR phenotype.

Original languageEnglish
Pages (from-to)245-253
Number of pages9
JournalActa Microbiologica et Immunologica Hungarica
Volume64
Issue number3
Early online date31 May 2017
DOIs
Publication statusPublished - 1 Sep 2017

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© Akadémiai Kiadó Zrt.

Keywords

  • Moxifloxacin
  • Multidrug resistance
  • NfxB
  • P. aeruginosa

Cite this

Thai, V. C., Pham, H. V. Y., Nguyen, D. N. M., Lambert, P., & Nguyen, T. T. H. (2017). A deletion mutation in nfxB of in vitro-induced moxifloxacin-resistant pseudomonas aeruginosa confers multidrug resistance. Acta Microbiologica et Immunologica Hungarica, 64(3), 245-253. https://doi.org/10.1556/030.64.2017.012