A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia

Christina Schilde, Thomas Wassmer, Joerg Mansfeld, Helmut Plattner, Roland Kissmehl

Research output: Contribution to journalArticle

Abstract

SNARE proteins (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) mediate membrane interactions and are conventionally divided into Q-SNAREs and R-SNAREs according to the possession of a glutamine or arginine residue at the core of their SNARE domain. Here, we describe a set of R-SNAREs from the ciliate Paramecium tetraurelia consisting of seven families encoded by 12 genes that are expressed simultaneously. The complexity of the endomembrane system in Paramecium can explain this high number of genes. All P. tetraurelia synaptobrevins (PtSybs) possess a SNARE domain and show homology to the Longin family of R-SNAREs such as Ykt6, Sec22 and tetanus toxin-insensitive VAMP (TI-VAMP). We localized four exemplary PtSyb subfamilies with GFP constructs and antibodies on the light and electron microscopic level. PtSyb1-1, PtSyb1-2 and PtSyb3-1 were found in the endoplasmic reticulum, whereas PtSyb2 is localized exclusively in the contractile vacuole complex. PtSyb6 was found cytosolic but also resides in regularly arranged structures at the cell cortex (parasomal sacs), the cytoproct and oral apparatus, probably representing endocytotic compartments. With gene silencing, we showed that the R-SNARE of the contractile vacuole complex, PtSyb2, functions to maintain structural integrity as well as functionality of the osmoregulatory system but also affects cell division.
Original languageEnglish
Pages (from-to)440-455
Number of pages16
JournalTraffic
Volume7
Issue number4
Early online date14 Mar 2006
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Paramecium tetraurelia
SNARE Proteins
Multigene Family
Genes
Vacuoles
Q-SNARE Proteins
R-SNARE Proteins
Paramecium
Tetanus Toxin
Gene Silencing
Structural integrity
Glutamine
Endoplasmic Reticulum
Cell Division
Arginine
Cells
Electrons
Membranes
Light

Keywords

  • membrane trafficking
  • ciliates
  • Paramecium
  • regulated secretion
  • SNAREs
  • R-SNAREs
  • v-SNAREs

Cite this

Schilde, C., Wassmer, T., Mansfeld, J., Plattner, H., & Kissmehl, R. (2006). A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia. Traffic, 7(4), 440-455. https://doi.org/10.1111/j.1600-0854.2006.00397.x
Schilde, Christina ; Wassmer, Thomas ; Mansfeld, Joerg ; Plattner, Helmut ; Kissmehl, Roland. / A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia. In: Traffic. 2006 ; Vol. 7, No. 4. pp. 440-455.
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Schilde, C, Wassmer, T, Mansfeld, J, Plattner, H & Kissmehl, R 2006, 'A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia', Traffic, vol. 7, no. 4, pp. 440-455. https://doi.org/10.1111/j.1600-0854.2006.00397.x

A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia. / Schilde, Christina; Wassmer, Thomas; Mansfeld, Joerg; Plattner, Helmut; Kissmehl, Roland.

In: Traffic, Vol. 7, No. 4, 04.2006, p. 440-455.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia

AU - Schilde, Christina

AU - Wassmer, Thomas

AU - Mansfeld, Joerg

AU - Plattner, Helmut

AU - Kissmehl, Roland

PY - 2006/4

Y1 - 2006/4

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KW - membrane trafficking

KW - ciliates

KW - Paramecium

KW - regulated secretion

KW - SNAREs

KW - R-SNAREs

KW - v-SNAREs

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