A novel serological test for the diagnosis of central venous catheter-associated sepsis

T. S J Elliott; S. E. Tebbs; H. A. Moss; T. Worthington; M. K. Spare; M. H. Faroqui; P. A. Lambert

doi:10.1053/jinf.2000.0656

A novel serological test for the diagnosis of central venous catheter-associated sepsis

T. S J Elliott^*, S. E. Tebbs, H. A. Moss, T. Worthington, M. K. Spare, M. H. Faroqui, P. A. Lambert

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: To determine the sensitivity and specificity of a novel antibody test for the diagnosis of intravascular catheter-related infections due to coagulase-negative staphylococci.

Methods: Sixty-seven patients diagnosed as having central venous catheter (CVC)-associated sepsis based on strict clinical criteria, including positive blood cultures, were compared to 67 patients with a CVC in situ who exhibited no evidence of sepsis. An ELISA serological test based on a novel short-chain lipoteichoic acid antigen isolated from coagulase-negative staphylococci (CNS) was used to determine the patient's serological response (IgG and IgM) to CVC sepsis caused by CMS. The specificity and sensitivity of the test was determined.

Results: There was a significant increase in the antibody levels (IgG and IgM) to the short-chain lipoteichoic acid in patients with CVC-associated staphylococcal sepsis as compared to the control patients.

Conclusions: This new serological method may offer a useful diagnostic test for intravascular catheter infections caused by staphylococci.

Original language	English
Pages (from-to)	262-266
Number of pages	5
Journal	Journal of Infection
Volume	40
Issue number	3
DOIs	https://doi.org/10.1053/jinf.2000.0656
Publication status	Published - May 2000

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title = "A novel serological test for the diagnosis of central venous catheter-associated sepsis",

abstract = "Objectives: To determine the sensitivity and specificity of a novel antibody test for the diagnosis of intravascular catheter-related infections due to coagulase-negative staphylococci. Methods: Sixty-seven patients diagnosed as having central venous catheter (CVC)-associated sepsis based on strict clinical criteria, including positive blood cultures, were compared to 67 patients with a CVC in situ who exhibited no evidence of sepsis. An ELISA serological test based on a novel short-chain lipoteichoic acid antigen isolated from coagulase-negative staphylococci (CNS) was used to determine the patient's serological response (IgG and IgM) to CVC sepsis caused by CMS. The specificity and sensitivity of the test was determined. Results: There was a significant increase in the antibody levels (IgG and IgM) to the short-chain lipoteichoic acid in patients with CVC-associated staphylococcal sepsis as compared to the control patients. Conclusions: This new serological method may offer a useful diagnostic test for intravascular catheter infections caused by staphylococci. ",

author = "Elliott, {T. S J} and Tebbs, {S. E.} and Moss, {H. A.} and T. Worthington and Spare, {M. K.} and Faroqui, {M. H.} and Lambert, {P. A.}",

year = "2000",

month = may,

doi = "10.1053/jinf.2000.0656",

language = "English",

volume = "40",

pages = "262--266",

journal = "Journal of Infection",

issn = "0163-4453",

publisher = "W.B. Saunders Ltd",

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TY - JOUR

T1 - A novel serological test for the diagnosis of central venous catheter-associated sepsis

AU - Elliott, T. S J

AU - Tebbs, S. E.

AU - Moss, H. A.

AU - Worthington, T.

AU - Spare, M. K.

AU - Faroqui, M. H.

AU - Lambert, P. A.

PY - 2000/5

Y1 - 2000/5

N2 - Objectives: To determine the sensitivity and specificity of a novel antibody test for the diagnosis of intravascular catheter-related infections due to coagulase-negative staphylococci. Methods: Sixty-seven patients diagnosed as having central venous catheter (CVC)-associated sepsis based on strict clinical criteria, including positive blood cultures, were compared to 67 patients with a CVC in situ who exhibited no evidence of sepsis. An ELISA serological test based on a novel short-chain lipoteichoic acid antigen isolated from coagulase-negative staphylococci (CNS) was used to determine the patient's serological response (IgG and IgM) to CVC sepsis caused by CMS. The specificity and sensitivity of the test was determined. Results: There was a significant increase in the antibody levels (IgG and IgM) to the short-chain lipoteichoic acid in patients with CVC-associated staphylococcal sepsis as compared to the control patients. Conclusions: This new serological method may offer a useful diagnostic test for intravascular catheter infections caused by staphylococci.

AB - Objectives: To determine the sensitivity and specificity of a novel antibody test for the diagnosis of intravascular catheter-related infections due to coagulase-negative staphylococci. Methods: Sixty-seven patients diagnosed as having central venous catheter (CVC)-associated sepsis based on strict clinical criteria, including positive blood cultures, were compared to 67 patients with a CVC in situ who exhibited no evidence of sepsis. An ELISA serological test based on a novel short-chain lipoteichoic acid antigen isolated from coagulase-negative staphylococci (CNS) was used to determine the patient's serological response (IgG and IgM) to CVC sepsis caused by CMS. The specificity and sensitivity of the test was determined. Results: There was a significant increase in the antibody levels (IgG and IgM) to the short-chain lipoteichoic acid in patients with CVC-associated staphylococcal sepsis as compared to the control patients. Conclusions: This new serological method may offer a useful diagnostic test for intravascular catheter infections caused by staphylococci.

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UR - https://www.sciencedirect.com/science/article/pii/S016344530090656X?via%3Dihub

U2 - 10.1053/jinf.2000.0656

DO - 10.1053/jinf.2000.0656

M3 - Article

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AN - SCOPUS:0033936915

SN - 0163-4453

VL - 40

SP - 262

EP - 266

JO - Journal of Infection

JF - Journal of Infection

IS - 3

ER -

A novel serological test for the diagnosis of central venous catheter-associated sepsis

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