A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro

Mike Coleman, John O'Neil, Elizabeth Woehrling, Oscar Ndunge, Eric Hill, Andre Menache, Claud Reiss

Research output: Contribution to journalArticle

Abstract

Many pesticides are used increasingly in combinations during crop protection and their stability ensures the presence of such combinations in foodstuffs. The effects of three fungicides, pyrimethanil, cyprodinil and fludioxonil, were investigated together and separately on U251 and SH-SY5Y cells, which can be representative of human CNS glial and neuronal cells respectively. Over 48h, all three agents showed significant reductions in cellular ATP, at concentrations that were more than tenfold lower than those which significantly impaired cellular viability. The effects on energy metabolism were reflected in their marked toxic effects on mitochondrial membrane potential. In addition, evidence of oxidative stress was seen in terms of a fall in cellular thiols coupled with increases in the expression of enzymes associated with reactive species formation, such as GSH peroxidase and superoxide dismutase. The glial cell line showed significant responsiveness to the toxin challenge in terms of changes in antioxidant gene expression, although the neuronal SH-SY5Y line exhibited greater vulnerability to toxicity, which was reflected in significant increases in caspase-3 expression, which is indicative of the initiation of apoptosis. Cyprodinil was the most toxic agent individually, although oxidative stress-related enzyme gene expression increases appeared to demonstrate some degree of synergy in the presence of the combination of agents. This report suggests that the impact of some pesticides, both individually and in combinations, merits further study in terms of their impact on human cellular health.
LanguageEnglish
Article numbere42768
Number of pages8
JournalPLoS ONE
Volume7
Issue number8
DOIs
Publication statusPublished - 3 Aug 2012

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cyprodinil
Poisons
neuroglia
Pesticides
Neuroglia
Oxidative Stress
Oxidative stress
oxidative stress
pesticides
neurons
cell lines
fludioxonil
Gene Expression
Gene expression
Cell Line
gene expression
Mitochondrial Membrane Potential
caspase-3
Enzymes
enzymes

Bibliographical note

© 2012 Coleman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Cite this

Coleman, Mike ; O'Neil, John ; Woehrling, Elizabeth ; Ndunge, Oscar ; Hill, Eric ; Menache, Andre ; Reiss, Claud. / A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro. In: PLoS ONE. 2012 ; Vol. 7, No. 8.
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A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro. / Coleman, Mike; O'Neil, John; Woehrling, Elizabeth; Ndunge, Oscar; Hill, Eric; Menache, Andre; Reiss, Claud.

In: PLoS ONE, Vol. 7, No. 8, e42768, 03.08.2012.

Research output: Contribution to journalArticle

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