A quantitative approach for understanding small-scale human mesenchymal stem cell culture - implications for large-scale bioprocess development

Qasim A. Rafiq, Karen Coopman, Alvin W. Nienow, Christopher J. Hewitt*

*Corresponding author for this work

Research output: Contribution to journalSpecial issue

Abstract

Human mesenchymal stem cell (hMSC) therapies have the potential to revolutionise the healthcare industry and replicate the success of the therapeutic protein industry; however, for this to be achieved there is a need to apply key bioprocessing engineering principles and adopt a quantitative approach for large-scale reproducible hMSC bioprocess development. Here we provide a quantitative analysis of the changes in concentration of glucose, lactate and ammonium with time during hMSC monolayer culture over 4 passages, under 100% and 20% dissolved oxgen (dO2), where either a 100%, 50% or 0% growth medium exchange was performed after 72h in culture. Yield coefficients, specific growth rates (h-1) and doubling times (h) were calculated for all cases. The 100% dO2 flasks outperformed the 20% dO2 flasks with respect to cumulative cell number, with the latter consuming more glucose and producing more lactate and ammonium. Furthermore, the 100% and 50% medium exchange conditions resulted in similar cumulative cell numbers, whilst the 0% conditions were significantly lower. Cell immunophenotype and multipotency were not affected by the experimental culture conditions. This study demonstrates the importance of determining optimal culture conditions for hMSC expansion and highlights a potential cost savings from only making a 50% medium exchange, which may prove significant for large-scale bioprocessing.

Original languageEnglish
Pages (from-to)459-471
Number of pages13
JournalBiotechnology Journal
Volume8
Issue number4
Early online date28 Feb 2013
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Mesenchymal Stromal Cells
Cell Culture Techniques
Lactic Acid
Cell Count
Glucose
Health Care Sector
Cost Savings
Growth
Cell- and Tissue-Based Therapy
Industry
Proteins
Therapeutics

Keywords

  • bioprocessing
  • human mesenchymal stem cells
  • hypoxia
  • normoxia
  • regenerative medicine

Cite this

Rafiq, Qasim A. ; Coopman, Karen ; Nienow, Alvin W. ; Hewitt, Christopher J. / A quantitative approach for understanding small-scale human mesenchymal stem cell culture - implications for large-scale bioprocess development. In: Biotechnology Journal. 2013 ; Vol. 8, No. 4. pp. 459-471.
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A quantitative approach for understanding small-scale human mesenchymal stem cell culture - implications for large-scale bioprocess development. / Rafiq, Qasim A.; Coopman, Karen; Nienow, Alvin W.; Hewitt, Christopher J.

In: Biotechnology Journal, Vol. 8, No. 4, 04.2013, p. 459-471.

Research output: Contribution to journalSpecial issue

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