Laser speckle imaging (LSI) is a well-known and useful approach for the non-invasive visualization of flows and microcirculation localized in turbid scattering media, including biological tissues (such as brain vasculature, skin capillaries etc.). Despite an extensive use of LSI for brain imaging, the LSI technique has several critical limitations. One of them is associated with inability to resolve a functionality of vessels. This limitation also leads to the systematic error in the quantitative interpretation of values of speckle contrast obtained for different vessel types, such as sagittal sinus, arteries, and veins. Here, utilizing a combined use of LSI and fluorescent intravital microscopy (FIM), we present a simple and robust method to overcome the limitations mentioned above for the LSI approach. The proposed technique provides more relevant, abundant, and valuable information regarding perfusion rate ration between different types of vessels that makes this method highly useful for in vivo brain surgical operations.
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Funding: The Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics (Sta Scientists grant program), Academy of Finland (project 326204), NATO (project SPS G5147), MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005), National Research Tomsk State University Academic D.I. Mendeleev Fund Program, Finnish Cultural Foundation (00180998) grant.
- Cerebral blood vessels
- In vivo fluorescence imaging
- Middle cerebral artery
- Non-invasive optical imaging
- Speckle contrast
- Transcranial imaging