A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules

Richard J. Goodwin, L. Macintyre, D.G. Watson, S.P. Scullion, Andrew Pitt

Research output: Contribution to journalArticle

Abstract

Matrix application continues to be a critical step in sample preparation for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI). Imaging of small molecules such as drugs and metabolites is particularly problematic because the commonly used washing steps to remove salts are usually omitted as they may also remove the analyte, and analyte spreading is more likely with conventional wet matrix application methods. We have developed a method which uses the application of matrix as a dry, finely divided powder, here referred to as dry matrix application, for the imaging of drug compounds. This appears to offer a complementary method to wet matrix application for the MALDI-MSI of small molecules, with the alternative matrix application techniques producing different ion profiles, and allows the visualization of compounds not observed using wet matrix application methods. We demonstrate its value in imaging clozapine from rat kidney and 4-bromophenyl-1,4-diazabicyclo(3.2.2)nonane-4-carboxylic acid from rat brain. In addition, exposure of the dry matrix coated sample to a saturated moist atmosphere appears to enhance the visualization of a different set of molecules.
LanguageEnglish
Pages1682-1686
Number of pages5
JournalRapid communications in mass spectrometry
Volume24
Issue number11
Early online date10 May 2010
DOIs
Publication statusPublished - 15 Jun 2010

Fingerprint

Ionization
Desorption
Imaging techniques
Molecules
Lasers
Mass spectrometry
Rats
Visualization
Clozapine
Metabolites
Carboxylic Acids
Washing
Pharmaceutical Preparations
Powders
Brain
Salts
Ions

Keywords

  • animals
  • heterocyclic bicyclo compounds
  • brain chemistry
  • clozapine
  • kidney
  • male
  • rats
  • inbred BB rats
  • Wistar rats
  • spectrometry
  • matrix-assisted laser
  • desorption-ionization
  • mass

Cite this

Goodwin, Richard J. ; Macintyre, L. ; Watson, D.G. ; Scullion, S.P. ; Pitt, Andrew. / A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules. In: Rapid communications in mass spectrometry. 2010 ; Vol. 24, No. 11. pp. 1682-1686.
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A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules. / Goodwin, Richard J.; Macintyre, L.; Watson, D.G.; Scullion, S.P.; Pitt, Andrew.

In: Rapid communications in mass spectrometry, Vol. 24, No. 11, 15.06.2010, p. 1682-1686.

Research output: Contribution to journalArticle

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