Accommodative response to electrical stimulation of the sclera of peripheral cornea in cats and porcines

Toshifumi Mihashi, Yoko Hirohara, Tomomitsu Miyoshi, Suguru Miyagawa, Hiroyuki Kanda, Hajime Sawai, Takashi Fujikado, Thomas Drew, James Stuart Wolffsohn

Research output: Contribution to journalMeeting abstract

Abstract

Purpose: We have reported that the changes in the accommodative response to electrical stimulation of the branches of the ciliary nerves in cats. (Miyagawa et al, PLoS One, 2014). We have also reported that no robust accommodative responses to the electrical stimulations of the sclera of peripheral cornea (SSPC) were observed in enucleated porcine eyes (Mihashi et al, VPOptics, 2014). In this study, accommodative responses to SSPC stimulation in cats and porcines were investigated.
Methods: Two eyes of two cats under anesthesia and after they were sacrificed were studied. Three enucleated porcine eyes obtained from a local slaughterhouse were also studied. Trains of biphasic pulses (current, 3 mA; duration, 2 ms/phase; frequency, 40 Hz) were applied using a tungsten electrode (0.3mm diameter) from several orientations. Wavefront sensing with a compact wavefront aberrometer (Uday et al J Cataract Refract Surg, 2013) were performed before and 4 s (cat) and 10 s (pig) after the stimulations and wavefront aberrations including spherical errors were analyzed over a 4-mm pupil area.
Results: In the first cat under anesthesia, at three out of seven stimulus positions, 0.2 D hyperopic accommodative responses were observed and in two orientations, myopic responses were observed. For the other cat, weak accommodative responses including astigmatic changes were observed. In the sacrificed condition of the second cat, 0.1 D myopic response was observed for one stimulus orientation and the smaller responses were observed at six out of eight stimulus positions. No accommodative responses were elicited for the enucleated porcine eyes.
Conclusions: In the anesthetized cats, electrical stimulation of the SSPC induced accommodative responses; the responses were unstable and weaker than the responses by the ciliary nerve stimulations we observed in our previous study. Small accommodative responses were observed after one of two cats had been sacrificed, but no accommodative responses were detected in the enucleated porcine eyes. Further studies are needed to confirm difference in the accommodation functions in the two species.
Original languageEnglish
Pages (from-to)6005
Number of pages1
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number7
Publication statusPublished - Jun 2015
EventARVO 2015 Annual Meeting : Powerful Connections: Vision Research and Online Networking - Colorado Convention Center (CCC), Denver, CO, United States
Duration: 2 May 20157 May 2015

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Sclera
Cornea
Electric Stimulation
Cats
Swine
Anesthesia
Tungsten
Abattoirs
Pupil
Cataract
Electrodes

Bibliographical note

ARVO 2015 Annual Meeting : Powerful Connections: Vision Research and Online Networking, 2-7 May 2015, Denver, CO, United States.

Cite this

Mihashi, Toshifumi ; Hirohara, Yoko ; Miyoshi, Tomomitsu ; Miyagawa, Suguru ; Kanda, Hiroyuki ; Sawai, Hajime ; Fujikado, Takashi ; Drew, Thomas ; Wolffsohn, James Stuart. / Accommodative response to electrical stimulation of the sclera of peripheral cornea in cats and porcines. In: Investigative Ophthalmology and Visual Science. 2015 ; Vol. 56, No. 7. pp. 6005.
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title = "Accommodative response to electrical stimulation of the sclera of peripheral cornea in cats and porcines",
abstract = "Purpose: We have reported that the changes in the accommodative response to electrical stimulation of the branches of the ciliary nerves in cats. (Miyagawa et al, PLoS One, 2014). We have also reported that no robust accommodative responses to the electrical stimulations of the sclera of peripheral cornea (SSPC) were observed in enucleated porcine eyes (Mihashi et al, VPOptics, 2014). In this study, accommodative responses to SSPC stimulation in cats and porcines were investigated. Methods: Two eyes of two cats under anesthesia and after they were sacrificed were studied. Three enucleated porcine eyes obtained from a local slaughterhouse were also studied. Trains of biphasic pulses (current, 3 mA; duration, 2 ms/phase; frequency, 40 Hz) were applied using a tungsten electrode (0.3mm diameter) from several orientations. Wavefront sensing with a compact wavefront aberrometer (Uday et al J Cataract Refract Surg, 2013) were performed before and 4 s (cat) and 10 s (pig) after the stimulations and wavefront aberrations including spherical errors were analyzed over a 4-mm pupil area. Results: In the first cat under anesthesia, at three out of seven stimulus positions, 0.2 D hyperopic accommodative responses were observed and in two orientations, myopic responses were observed. For the other cat, weak accommodative responses including astigmatic changes were observed. In the sacrificed condition of the second cat, 0.1 D myopic response was observed for one stimulus orientation and the smaller responses were observed at six out of eight stimulus positions. No accommodative responses were elicited for the enucleated porcine eyes.Conclusions: In the anesthetized cats, electrical stimulation of the SSPC induced accommodative responses; the responses were unstable and weaker than the responses by the ciliary nerve stimulations we observed in our previous study. Small accommodative responses were observed after one of two cats had been sacrificed, but no accommodative responses were detected in the enucleated porcine eyes. Further studies are needed to confirm difference in the accommodation functions in the two species.",
author = "Toshifumi Mihashi and Yoko Hirohara and Tomomitsu Miyoshi and Suguru Miyagawa and Hiroyuki Kanda and Hajime Sawai and Takashi Fujikado and Thomas Drew and Wolffsohn, {James Stuart}",
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Mihashi, T, Hirohara, Y, Miyoshi, T, Miyagawa, S, Kanda, H, Sawai, H, Fujikado, T, Drew, T & Wolffsohn, JS 2015, 'Accommodative response to electrical stimulation of the sclera of peripheral cornea in cats and porcines', Investigative Ophthalmology and Visual Science, vol. 56, no. 7, pp. 6005.

Accommodative response to electrical stimulation of the sclera of peripheral cornea in cats and porcines. / Mihashi, Toshifumi; Hirohara, Yoko; Miyoshi, Tomomitsu; Miyagawa, Suguru; Kanda, Hiroyuki; Sawai, Hajime; Fujikado, Takashi; Drew, Thomas; Wolffsohn, James Stuart.

In: Investigative Ophthalmology and Visual Science, Vol. 56, No. 7, 06.2015, p. 6005.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Accommodative response to electrical stimulation of the sclera of peripheral cornea in cats and porcines

AU - Mihashi, Toshifumi

AU - Hirohara, Yoko

AU - Miyoshi, Tomomitsu

AU - Miyagawa, Suguru

AU - Kanda, Hiroyuki

AU - Sawai, Hajime

AU - Fujikado, Takashi

AU - Drew, Thomas

AU - Wolffsohn, James Stuart

N1 - ARVO 2015 Annual Meeting : Powerful Connections: Vision Research and Online Networking, 2-7 May 2015, Denver, CO, United States.

PY - 2015/6

Y1 - 2015/6

N2 - Purpose: We have reported that the changes in the accommodative response to electrical stimulation of the branches of the ciliary nerves in cats. (Miyagawa et al, PLoS One, 2014). We have also reported that no robust accommodative responses to the electrical stimulations of the sclera of peripheral cornea (SSPC) were observed in enucleated porcine eyes (Mihashi et al, VPOptics, 2014). In this study, accommodative responses to SSPC stimulation in cats and porcines were investigated. Methods: Two eyes of two cats under anesthesia and after they were sacrificed were studied. Three enucleated porcine eyes obtained from a local slaughterhouse were also studied. Trains of biphasic pulses (current, 3 mA; duration, 2 ms/phase; frequency, 40 Hz) were applied using a tungsten electrode (0.3mm diameter) from several orientations. Wavefront sensing with a compact wavefront aberrometer (Uday et al J Cataract Refract Surg, 2013) were performed before and 4 s (cat) and 10 s (pig) after the stimulations and wavefront aberrations including spherical errors were analyzed over a 4-mm pupil area. Results: In the first cat under anesthesia, at three out of seven stimulus positions, 0.2 D hyperopic accommodative responses were observed and in two orientations, myopic responses were observed. For the other cat, weak accommodative responses including astigmatic changes were observed. In the sacrificed condition of the second cat, 0.1 D myopic response was observed for one stimulus orientation and the smaller responses were observed at six out of eight stimulus positions. No accommodative responses were elicited for the enucleated porcine eyes.Conclusions: In the anesthetized cats, electrical stimulation of the SSPC induced accommodative responses; the responses were unstable and weaker than the responses by the ciliary nerve stimulations we observed in our previous study. Small accommodative responses were observed after one of two cats had been sacrificed, but no accommodative responses were detected in the enucleated porcine eyes. Further studies are needed to confirm difference in the accommodation functions in the two species.

AB - Purpose: We have reported that the changes in the accommodative response to electrical stimulation of the branches of the ciliary nerves in cats. (Miyagawa et al, PLoS One, 2014). We have also reported that no robust accommodative responses to the electrical stimulations of the sclera of peripheral cornea (SSPC) were observed in enucleated porcine eyes (Mihashi et al, VPOptics, 2014). In this study, accommodative responses to SSPC stimulation in cats and porcines were investigated. Methods: Two eyes of two cats under anesthesia and after they were sacrificed were studied. Three enucleated porcine eyes obtained from a local slaughterhouse were also studied. Trains of biphasic pulses (current, 3 mA; duration, 2 ms/phase; frequency, 40 Hz) were applied using a tungsten electrode (0.3mm diameter) from several orientations. Wavefront sensing with a compact wavefront aberrometer (Uday et al J Cataract Refract Surg, 2013) were performed before and 4 s (cat) and 10 s (pig) after the stimulations and wavefront aberrations including spherical errors were analyzed over a 4-mm pupil area. Results: In the first cat under anesthesia, at three out of seven stimulus positions, 0.2 D hyperopic accommodative responses were observed and in two orientations, myopic responses were observed. For the other cat, weak accommodative responses including astigmatic changes were observed. In the sacrificed condition of the second cat, 0.1 D myopic response was observed for one stimulus orientation and the smaller responses were observed at six out of eight stimulus positions. No accommodative responses were elicited for the enucleated porcine eyes.Conclusions: In the anesthetized cats, electrical stimulation of the SSPC induced accommodative responses; the responses were unstable and weaker than the responses by the ciliary nerve stimulations we observed in our previous study. Small accommodative responses were observed after one of two cats had been sacrificed, but no accommodative responses were detected in the enucleated porcine eyes. Further studies are needed to confirm difference in the accommodation functions in the two species.

M3 - Meeting abstract

VL - 56

SP - 6005

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 1552-5783

IS - 7

ER -