Acquisition and retention of Clostridium difficile by Musca domestica larvae and pupae during metamorphosis

Matthew Davies, Moray Anderson, Anthony C. Hilton

Research output: Contribution to journalArticle

Abstract

Background: Transfer of Clostridium difficile by Musca domestica has been demonstrated, revealing their potential for disseminating infection in the hospital environment.
Aim: To determine the ability of M. domestica larvae to acquire and retain C. difficile throughout their metamorphosis into adult flies.
Methods: Larvae were exposed to spores of C. difficile in a faecal emulsion and examined externally and internally to determine carriage and internalisation of spores through their development to adults.
Findings: Larvae harboured C. difficile externally, with means of 21.56 +/- 5.76 CFUs at day zero, 22.44 +/- 9.90 after two days, decreasing to 0.56 +/- 0.34 at day four, with no C. difficile isolated thereafter. The same larvae harboured C. difficile internally, with means of 587.33 +/- 238.29 CFUs at day zero, decreasing to 297.44 +/- 155.21 after two days, decreasing further to 73.67 +/- 46.74 after four days, with no C. difficile isolated thereafter. The zero recovery of C. difficile coincided with the development of M. domestica larvae into pupae. From day six onwards, all larvae had developed into the pupal stage and no C. difficile was recoverable from any pupae. No C. difficile was recovered from adult flies (emerged on day twelve), or empty puparia.
Conclusion: Although C. difficile spores are readily acquired and internalised by larvae during feeding, they are not retained through development to adults. Adult flies therefore acquire C. difficile contamination as adults. The potential antimicrobial action of M. domestica larvae and their extracts against C. difficile spores warrants further investigation.
Original languageEnglish
Pages (from-to)410-414
JournalJournal of Hospital Infection
Volume95
Issue number4
Early online date27 Jan 2017
DOIs
Publication statusPublished - 1 Apr 2017

Fingerprint

Houseflies
Pupa
Clostridium difficile
Larva
Spores
Diptera
Cross Infection
Emulsions
Action Potentials

Bibliographical note

© 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • musca domestica
  • housefly
  • pest control
  • infection control
  • clostridium difficile

Cite this

@article{c2caa48b58ba4c20861d81993652dbb9,
title = "Acquisition and retention of Clostridium difficile by Musca domestica larvae and pupae during metamorphosis",
abstract = "Background: Transfer of Clostridium difficile by Musca domestica has been demonstrated, revealing their potential for disseminating infection in the hospital environment. Aim: To determine the ability of M. domestica larvae to acquire and retain C. difficile throughout their metamorphosis into adult flies. Methods: Larvae were exposed to spores of C. difficile in a faecal emulsion and examined externally and internally to determine carriage and internalisation of spores through their development to adults. Findings: Larvae harboured C. difficile externally, with means of 21.56 +/- 5.76 CFUs at day zero, 22.44 +/- 9.90 after two days, decreasing to 0.56 +/- 0.34 at day four, with no C. difficile isolated thereafter. The same larvae harboured C. difficile internally, with means of 587.33 +/- 238.29 CFUs at day zero, decreasing to 297.44 +/- 155.21 after two days, decreasing further to 73.67 +/- 46.74 after four days, with no C. difficile isolated thereafter. The zero recovery of C. difficile coincided with the development of M. domestica larvae into pupae. From day six onwards, all larvae had developed into the pupal stage and no C. difficile was recoverable from any pupae. No C. difficile was recovered from adult flies (emerged on day twelve), or empty puparia.Conclusion: Although C. difficile spores are readily acquired and internalised by larvae during feeding, they are not retained through development to adults. Adult flies therefore acquire C. difficile contamination as adults. The potential antimicrobial action of M. domestica larvae and their extracts against C. difficile spores warrants further investigation.",
keywords = "musca domestica, housefly, pest control, infection control, clostridium difficile",
author = "Matthew Davies and Moray Anderson and Hilton, {Anthony C.}",
note = "{\circledC} 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/",
year = "2017",
month = "4",
day = "1",
doi = "10.1016/j.jhin.2017.01.010",
language = "English",
volume = "95",
pages = "410--414",
journal = "Journal of Hospital Infection",
issn = "0195-6701",
publisher = "W.B. Saunders Ltd",
number = "4",

}

Acquisition and retention of Clostridium difficile by Musca domestica larvae and pupae during metamorphosis. / Davies, Matthew; Anderson, Moray; Hilton, Anthony C.

In: Journal of Hospital Infection, Vol. 95, No. 4, 01.04.2017, p. 410-414.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Acquisition and retention of Clostridium difficile by Musca domestica larvae and pupae during metamorphosis

AU - Davies, Matthew

AU - Anderson, Moray

AU - Hilton, Anthony C.

N1 - © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Background: Transfer of Clostridium difficile by Musca domestica has been demonstrated, revealing their potential for disseminating infection in the hospital environment. Aim: To determine the ability of M. domestica larvae to acquire and retain C. difficile throughout their metamorphosis into adult flies. Methods: Larvae were exposed to spores of C. difficile in a faecal emulsion and examined externally and internally to determine carriage and internalisation of spores through their development to adults. Findings: Larvae harboured C. difficile externally, with means of 21.56 +/- 5.76 CFUs at day zero, 22.44 +/- 9.90 after two days, decreasing to 0.56 +/- 0.34 at day four, with no C. difficile isolated thereafter. The same larvae harboured C. difficile internally, with means of 587.33 +/- 238.29 CFUs at day zero, decreasing to 297.44 +/- 155.21 after two days, decreasing further to 73.67 +/- 46.74 after four days, with no C. difficile isolated thereafter. The zero recovery of C. difficile coincided with the development of M. domestica larvae into pupae. From day six onwards, all larvae had developed into the pupal stage and no C. difficile was recoverable from any pupae. No C. difficile was recovered from adult flies (emerged on day twelve), or empty puparia.Conclusion: Although C. difficile spores are readily acquired and internalised by larvae during feeding, they are not retained through development to adults. Adult flies therefore acquire C. difficile contamination as adults. The potential antimicrobial action of M. domestica larvae and their extracts against C. difficile spores warrants further investigation.

AB - Background: Transfer of Clostridium difficile by Musca domestica has been demonstrated, revealing their potential for disseminating infection in the hospital environment. Aim: To determine the ability of M. domestica larvae to acquire and retain C. difficile throughout their metamorphosis into adult flies. Methods: Larvae were exposed to spores of C. difficile in a faecal emulsion and examined externally and internally to determine carriage and internalisation of spores through their development to adults. Findings: Larvae harboured C. difficile externally, with means of 21.56 +/- 5.76 CFUs at day zero, 22.44 +/- 9.90 after two days, decreasing to 0.56 +/- 0.34 at day four, with no C. difficile isolated thereafter. The same larvae harboured C. difficile internally, with means of 587.33 +/- 238.29 CFUs at day zero, decreasing to 297.44 +/- 155.21 after two days, decreasing further to 73.67 +/- 46.74 after four days, with no C. difficile isolated thereafter. The zero recovery of C. difficile coincided with the development of M. domestica larvae into pupae. From day six onwards, all larvae had developed into the pupal stage and no C. difficile was recoverable from any pupae. No C. difficile was recovered from adult flies (emerged on day twelve), or empty puparia.Conclusion: Although C. difficile spores are readily acquired and internalised by larvae during feeding, they are not retained through development to adults. Adult flies therefore acquire C. difficile contamination as adults. The potential antimicrobial action of M. domestica larvae and their extracts against C. difficile spores warrants further investigation.

KW - musca domestica

KW - housefly

KW - pest control

KW - infection control

KW - clostridium difficile

UR - http://www.scopus.com/inward/record.url?scp=85013377938&partnerID=8YFLogxK

UR - https://www.sciencedirect.com/science/article/pii/S0195670117300166?via%3Dihub

U2 - 10.1016/j.jhin.2017.01.010

DO - 10.1016/j.jhin.2017.01.010

M3 - Article

VL - 95

SP - 410

EP - 414

JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

SN - 0195-6701

IS - 4

ER -