@inbook{8fc1efa67d354f179231e43d4d7719bc,
title = "Acquisition of temporal HIF transcriptional activity using a secreted luciferase assay",
abstract = "Here we describe a simple method based on secreted luciferase driven by a hypoxia-inducible factor (HIF) response element (HRE) that allows the acquisition of dynamic and high-throughput data on HIF transcriptional activity during hypoxia and pharmacological activation of HIF. The sensitivity of the assay allows for the secreted luciferase to be consecutively sampled (as little as 1% of the total supernatant) over an extended time period, thus allowing the acquisition of time-resolved HIF transcriptional activity.",
keywords = "Activity, HIF, Hypoxia, Luciferase, Time-resolved, Transcription",
author = "Cavadas, {Miguel A.S.} and Taylor, {Cormac T.} and Alex Cheong",
year = "2018",
month = jan,
day = "13",
doi = "10.1007/978-1-4939-7665-2_4",
language = "English",
volume = "1742",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "37--44",
booktitle = "Methods in Molecular Biology",
address = "United States",
}