@inbook{8fc1efa67d354f179231e43d4d7719bc,
title = "Acquisition of temporal HIF transcriptional activity using a secreted luciferase assay",
abstract = "Here we describe a simple method based on secreted luciferase driven by a hypoxia-inducible factor (HIF) response element (HRE) that allows the acquisition of dynamic and high-throughput data on HIF transcriptional activity during hypoxia and pharmacological activation of HIF. The sensitivity of the assay allows for the secreted luciferase to be consecutively sampled (as little as 1\% of the total supernatant) over an extended time period, thus allowing the acquisition of time-resolved HIF transcriptional activity.",
keywords = "Activity, HIF, Hypoxia, Luciferase, Time-resolved, Transcription",
author = "Cavadas, \{Miguel A.S.\} and Taylor, \{Cormac T.\} and Alex Cheong",
year = "2018",
month = jan,
day = "13",
doi = "10.1007/978-1-4939-7665-2\_4",
language = "English",
volume = "1742",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "37--44",
booktitle = "Methods in Molecular Biology",
address = "United States",
}