Abstract
Induction of the Escherichia coli K-12 ynfEFGHI operon in response to anaerobiosis is repressed by nitrate ions. In this study, we show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element. Electromobility shift assays show that NarL does not prevent RNA polymerase binding and suggest that repression may involve a quaternary NarL-FNR-RNA polymerase-promoter complex.
| Original language | English |
|---|---|
| Pages (from-to) | 3172-3176 |
| Number of pages | 5 |
| Journal | Journal of Bacteriology |
| Volume | 191 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - May 2009 |
Keywords
- Anaerobiosis
- Base Sequence
- DNA-Binding Proteins/physiology
- DNA-Directed RNA Polymerases/metabolism
- Electrophoretic Mobility Shift Assay
- Escherichia coli K12/genetics
- Escherichia coli Proteins/physiology
- Gene Expression Regulation, Bacterial
- Iron-Sulfur Proteins/physiology
- Molecular Sequence Data
- Operon
- Promoter Regions, Genetic
- Protein Binding
- Transcriptional Activation