Active membrane transport and receptor proteins from bacteria

M. Saidijam, K.E. Bettaney, G. Szakonyi, G. Psakis, K. Shibayama, S. Suzuki, J.L. Clough, V. Blessie, A. Abu-bakr, S. Baumberg, J. Meuller, C.K. Hoyle, S.L. Palmer, P. Butaye, K. Walravens, S.G. Patching, J. O'Reilly, NG. Rutherford, Roslyn M. Bill, D.I. Roper & 2 others M.K. Phillips-Jones, P.J.F. Henderson

Research output: Contribution to journalArticle

Abstract

A general strategy for the expression of bacterial membrane transport and receptor genes in Escherichia coli is described. Expression is amplified so that the encoded proteins comprise 5-35% of E. coli inner membrane protein. Depending upon their topology, proteins are produced with RGSH6 or a Strep tag at the C-terminus. These enable purification in mg quantities for crystallization and NMR studies. Examples of one nutrient uptake and one multidrug extrusion protein from Helicobacter pylori are described. This strategy is successful for membrane proteins from H. pylori, E. coli, Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Microbacterium liquefaciens, Brucella abortus, Brucella melitensis, Campylobacter jejuni, Neisseria meningitides, Streptomyces coelicolor and Rhodobacter sphaeroides. ©2005 Biochemical Society.

Original languageEnglish
Pages (from-to)867-872
Number of pages6
JournalBiochemical Society Transactions
Volume33
Issue numberPart 4
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Membrane Transport Proteins
Active Biological Transport
Bacteria
Membranes
Helicobacter pylori
Escherichia coli
Brucella melitensis
Streptomyces coelicolor
Neisseria
Brucella abortus
Rhodobacter sphaeroides
Proteins
Campylobacter jejuni
Enterococcus faecalis
Bacilli
Crystallization
Bacillus subtilis
Meningitis
Nutrients
Purification

Bibliographical note

Copyright 2005 The Biochemical Society. The Accepted Manuscript is reproduced here in accordance with the copyright policy of the publisher. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from Portland Press Limited. The final Version of Record is available at http://www.biochemsoctrans.org Conference on Mechanisms of Bioenergetic Membrane Proteins - Structures and Beyond, Wiesbaden (DE), 20-24 March 2005

Keywords

  • bacteria
  • helicobacter pylori
  • his tag
  • membrane transport protein
  • pathogen
  • two-component system

Cite this

Saidijam, M., Bettaney, K. E., Szakonyi, G., Psakis, G., Shibayama, K., Suzuki, S., ... Henderson, P. J. F. (2005). Active membrane transport and receptor proteins from bacteria. Biochemical Society Transactions, 33(Part 4), 867-872. https://doi.org/10.1042/BST0330867
Saidijam, M. ; Bettaney, K.E. ; Szakonyi, G. ; Psakis, G. ; Shibayama, K. ; Suzuki, S. ; Clough, J.L. ; Blessie, V. ; Abu-bakr, A. ; Baumberg, S. ; Meuller, J. ; Hoyle, C.K. ; Palmer, S.L. ; Butaye, P. ; Walravens, K. ; Patching, S.G. ; O'Reilly, J. ; Rutherford, NG. ; Bill, Roslyn M. ; Roper, D.I. ; Phillips-Jones, M.K. ; Henderson, P.J.F. / Active membrane transport and receptor proteins from bacteria. In: Biochemical Society Transactions. 2005 ; Vol. 33, No. Part 4. pp. 867-872.
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Saidijam, M, Bettaney, KE, Szakonyi, G, Psakis, G, Shibayama, K, Suzuki, S, Clough, JL, Blessie, V, Abu-bakr, A, Baumberg, S, Meuller, J, Hoyle, CK, Palmer, SL, Butaye, P, Walravens, K, Patching, SG, O'Reilly, J, Rutherford, NG, Bill, RM, Roper, DI, Phillips-Jones, MK & Henderson, PJF 2005, 'Active membrane transport and receptor proteins from bacteria', Biochemical Society Transactions, vol. 33, no. Part 4, pp. 867-872. https://doi.org/10.1042/BST0330867

Active membrane transport and receptor proteins from bacteria. / Saidijam, M.; Bettaney, K.E.; Szakonyi, G.; Psakis, G.; Shibayama, K.; Suzuki, S.; Clough, J.L.; Blessie, V.; Abu-bakr, A.; Baumberg, S.; Meuller, J.; Hoyle, C.K.; Palmer, S.L.; Butaye, P.; Walravens, K.; Patching, S.G.; O'Reilly, J.; Rutherford, NG.; Bill, Roslyn M.; Roper, D.I.; Phillips-Jones, M.K.; Henderson, P.J.F.

In: Biochemical Society Transactions, Vol. 33, No. Part 4, 08.2005, p. 867-872.

Research output: Contribution to journalArticle

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Saidijam M, Bettaney KE, Szakonyi G, Psakis G, Shibayama K, Suzuki S et al. Active membrane transport and receptor proteins from bacteria. Biochemical Society Transactions. 2005 Aug;33(Part 4):867-872. https://doi.org/10.1042/BST0330867