Advanced optical phantom mimicking microvascular and directed blood flow in mouse brain

Oleksii Sieryi; Anton Sdobnov; Igor Meglinski; Alexander Bykov

doi:10.1007/s13534-025-00508-1

Advanced optical phantom mimicking microvascular and directed blood flow in mouse brain

Oleksii Sieryi^*
, Anton Sdobnov
, Igor Meglinski
, Alexander Bykov

^*Corresponding author for this work

Oulun yliopisto

Research output: Contribution to journal › Article › peer-review

2 Citations (SciVal)

4 Downloads (Pure)

Abstract

The accurate replication of cerebral hemodynamics is essential for advancing neuroimaging techniques and preclinical research. This study presents a novel multi-component dynamic optical phantom designed to model the complex blood flow dynamics of the mouse brain. The phantom incorporates a static base mimicking skull optical properties, a porous medium infused with a blood-mimicking solution to simulate microvascular perfusion, and a directed flow channel representing large vessels such as the sagittal sinus. The phantom structure was characterized using laser speckle contrast imaging (LSCI) to assess its ability to replicate in vivo-like blood flow patterns. The results demonstrate strong quantitative agreement between the phantom and transcranial LSCI measurements of mouse brain hemodynamics. Our key findings highlight the influence of tissue-mimicking perfusion structures and optical attenuation properties on the blood flow index, validating the phantom as a reproducible and physiologically relevant model. This optically tunable and dynamically controllable platform provides a robust tool for calibrating neuroimaging technologies, validating new optical diagnostic techniques, and investigating cerebral blood flow regulation in preclinical studies.

Original language	English
Pages (from-to)	77-86
Number of pages	10
Journal	Biomedical Engineering Letters
Volume	16
Issue number	1
Early online date	17 Sept 2025
DOIs	https://doi.org/10.1007/s13534-025-00508-1
Publication status	Published - 1 Jan 2026

Bibliographical note

Copyright © The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Funding

The authors acknowledge the support provided by the COST Actions CA21159 (PhoBioS) and CA23125 (TETRA), funded by the European Cooperation in Science and Technology (COST). This work has been supported by the European Union Horizon 2020 research and innovation program under grant agreement No 863214. Open Access funding provided by University of Oulu (including Oulu University Hospital).

Funders	Funder number
European Cooperation in Science and Technology	CA23125, CA21159
European Union’s Horizon 2020 research and innovation program	863214

Keywords

Mouse brain
Laser speckle contrast imaging
Dynamic optical phantom
Hemodynamics

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10.1007/s13534-025-00508-1Licence: CC BY 4.0

Advanced optical phantom mimicking microvascular and directed blood flow in mouse brain
Copyright © The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
Final published version, 1.59 MBLicence: CC BY 4.0

Cite this

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abstract = "The accurate replication of cerebral hemodynamics is essential for advancing neuroimaging techniques and preclinical research. This study presents a novel multi-component dynamic optical phantom designed to model the complex blood flow dynamics of the mouse brain. The phantom incorporates a static base mimicking skull optical properties, a porous medium infused with a blood-mimicking solution to simulate microvascular perfusion, and a directed flow channel representing large vessels such as the sagittal sinus. The phantom structure was characterized using laser speckle contrast imaging (LSCI) to assess its ability to replicate in vivo-like blood flow patterns. The results demonstrate strong quantitative agreement between the phantom and transcranial LSCI measurements of mouse brain hemodynamics. Our key findings highlight the influence of tissue-mimicking perfusion structures and optical attenuation properties on the blood flow index, validating the phantom as a reproducible and physiologically relevant model. This optically tunable and dynamically controllable platform provides a robust tool for calibrating neuroimaging technologies, validating new optical diagnostic techniques, and investigating cerebral blood flow regulation in preclinical studies.",

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Advanced optical phantom mimicking microvascular and directed blood flow in mouse brain

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