Advances in methods for the determination of biologically relevant lipid peroxidation products

Corinne M. Spickett, Ingrid Wiswedel, Werner Siems, Kamelija Zarkovic, Neven Zarkovic

Research output: Contribution to journalArticle

Abstract

Lipid peroxidation is recognized to be an important contributor to many chronic diseases, especially those of an inflammatory pathology. In addition to their value as markers of oxidative damage, lipid peroxidation products have also been shown to have a wide variety of biological and cell signalling effects. In view of this, accurate and sensitive methods for the measurement of lipid peroxidation products are essential. Although some assays have been described for many years, improvements in protocols are continually being reported and, with recent advances in instrumentation and technology, highly specialized and informative techniques are increasingly used. This article gives an overview of the most currently used methods and then addresses the recent advances in some specific approaches. The focus is on analysis of oxysterols, F(2)-isoprostanes and oxidized phospholipids by gas chromatography or liquid chromatography mass spectrometry techniques and immunoassays for the detection of 4-hydroxynonenal.
LanguageEnglish
Pages1172-1202
Number of pages31
JournalFree Radical Research
Volume44
Issue number10
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Lipid Peroxidation
Lipids
Cell signaling
Isoprostanes
Liquid chromatography
Pathology
Immunoassay
Liquid Chromatography
Gas chromatography
Gas Chromatography
Mass spectrometry
Assays
Mass Spectrometry
Phospholipids
Chronic Disease
Technology
Oxysterols
4-hydroxy-2-nonenal

Keywords

  • aldehydes
  • animals
  • clinical chemistry tests
  • enzyme-linked immunosorbent assay
  • F2-isoprostanes
  • humans
  • immunohistochemistry
  • lipid peroxidation
  • mass spectrometry
  • oxidative stress
  • oxysterols
  • 4-hydroxynonenal
  • ELISA

Cite this

Spickett, Corinne M. ; Wiswedel, Ingrid ; Siems, Werner ; Zarkovic, Kamelija ; Zarkovic, Neven. / Advances in methods for the determination of biologically relevant lipid peroxidation products. In: Free Radical Research. 2010 ; Vol. 44, No. 10. pp. 1172-1202.
@article{0ad9bd294cf24f05b17bf8e61625538e,
title = "Advances in methods for the determination of biologically relevant lipid peroxidation products",
abstract = "Lipid peroxidation is recognized to be an important contributor to many chronic diseases, especially those of an inflammatory pathology. In addition to their value as markers of oxidative damage, lipid peroxidation products have also been shown to have a wide variety of biological and cell signalling effects. In view of this, accurate and sensitive methods for the measurement of lipid peroxidation products are essential. Although some assays have been described for many years, improvements in protocols are continually being reported and, with recent advances in instrumentation and technology, highly specialized and informative techniques are increasingly used. This article gives an overview of the most currently used methods and then addresses the recent advances in some specific approaches. The focus is on analysis of oxysterols, F(2)-isoprostanes and oxidized phospholipids by gas chromatography or liquid chromatography mass spectrometry techniques and immunoassays for the detection of 4-hydroxynonenal.",
keywords = "aldehydes, animals, clinical chemistry tests, enzyme-linked immunosorbent assay, F2-isoprostanes, humans, immunohistochemistry, lipid peroxidation, mass spectrometry, oxidative stress, oxysterols, 4-hydroxynonenal, ELISA",
author = "Spickett, {Corinne M.} and Ingrid Wiswedel and Werner Siems and Kamelija Zarkovic and Neven Zarkovic",
year = "2010",
month = "10",
doi = "10.3109/10715762.2010.498476",
language = "English",
volume = "44",
pages = "1172--1202",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Informa Healthcare",
number = "10",

}

Advances in methods for the determination of biologically relevant lipid peroxidation products. / Spickett, Corinne M.; Wiswedel, Ingrid; Siems, Werner; Zarkovic, Kamelija; Zarkovic, Neven.

In: Free Radical Research, Vol. 44, No. 10, 10.2010, p. 1172-1202.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Advances in methods for the determination of biologically relevant lipid peroxidation products

AU - Spickett, Corinne M.

AU - Wiswedel, Ingrid

AU - Siems, Werner

AU - Zarkovic, Kamelija

AU - Zarkovic, Neven

PY - 2010/10

Y1 - 2010/10

N2 - Lipid peroxidation is recognized to be an important contributor to many chronic diseases, especially those of an inflammatory pathology. In addition to their value as markers of oxidative damage, lipid peroxidation products have also been shown to have a wide variety of biological and cell signalling effects. In view of this, accurate and sensitive methods for the measurement of lipid peroxidation products are essential. Although some assays have been described for many years, improvements in protocols are continually being reported and, with recent advances in instrumentation and technology, highly specialized and informative techniques are increasingly used. This article gives an overview of the most currently used methods and then addresses the recent advances in some specific approaches. The focus is on analysis of oxysterols, F(2)-isoprostanes and oxidized phospholipids by gas chromatography or liquid chromatography mass spectrometry techniques and immunoassays for the detection of 4-hydroxynonenal.

AB - Lipid peroxidation is recognized to be an important contributor to many chronic diseases, especially those of an inflammatory pathology. In addition to their value as markers of oxidative damage, lipid peroxidation products have also been shown to have a wide variety of biological and cell signalling effects. In view of this, accurate and sensitive methods for the measurement of lipid peroxidation products are essential. Although some assays have been described for many years, improvements in protocols are continually being reported and, with recent advances in instrumentation and technology, highly specialized and informative techniques are increasingly used. This article gives an overview of the most currently used methods and then addresses the recent advances in some specific approaches. The focus is on analysis of oxysterols, F(2)-isoprostanes and oxidized phospholipids by gas chromatography or liquid chromatography mass spectrometry techniques and immunoassays for the detection of 4-hydroxynonenal.

KW - aldehydes

KW - animals

KW - clinical chemistry tests

KW - enzyme-linked immunosorbent assay

KW - F2-isoprostanes

KW - humans

KW - immunohistochemistry

KW - lipid peroxidation

KW - mass spectrometry

KW - oxidative stress

KW - oxysterols

KW - 4-hydroxynonenal

KW - ELISA

UR - http://www.scopus.com/inward/record.url?scp=77956567906&partnerID=8YFLogxK

U2 - 10.3109/10715762.2010.498476

DO - 10.3109/10715762.2010.498476

M3 - Article

VL - 44

SP - 1172

EP - 1202

JO - Free Radical Research

T2 - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 10

ER -