Advances in methods for the determination of biologically relevant lipid peroxidation products

Corinne M. Spickett, Ingrid Wiswedel, Werner Siems, Kamelija Zarkovic, Neven Zarkovic

Research output: Contribution to journalArticle

Abstract

Lipid peroxidation is recognized to be an important contributor to many chronic diseases, especially those of an inflammatory pathology. In addition to their value as markers of oxidative damage, lipid peroxidation products have also been shown to have a wide variety of biological and cell signalling effects. In view of this, accurate and sensitive methods for the measurement of lipid peroxidation products are essential. Although some assays have been described for many years, improvements in protocols are continually being reported and, with recent advances in instrumentation and technology, highly specialized and informative techniques are increasingly used. This article gives an overview of the most currently used methods and then addresses the recent advances in some specific approaches. The focus is on analysis of oxysterols, F(2)-isoprostanes and oxidized phospholipids by gas chromatography or liquid chromatography mass spectrometry techniques and immunoassays for the detection of 4-hydroxynonenal.
Original languageEnglish
Pages (from-to)1172-1202
Number of pages31
JournalFree Radical Research
Volume44
Issue number10
DOIs
Publication statusPublished - Oct 2010

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Keywords

  • aldehydes
  • animals
  • clinical chemistry tests
  • enzyme-linked immunosorbent assay
  • F2-isoprostanes
  • humans
  • immunohistochemistry
  • lipid peroxidation
  • mass spectrometry
  • oxidative stress
  • oxysterols
  • 4-hydroxynonenal
  • ELISA

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