Abstract
Original language | English |
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Pages (from-to) | 215-220 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 347 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - 27 Jun 1994 |
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Keywords
- NMR
- antioxidant defense
- glutathione
- N-acetylcysteine
- captopril
- human erythrocyte
- Pharmacy and materia medica
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Alteration of the erythrocyte glutathione redox balance by N-acetylcysteine, captopril and exogenous glutathione. / Russell, Jim ; Spickett, Corinne M.; Reglinski, John ; Smith, W. Ewen ; McMurray, John ; Abdullah, Ibrahim B. .
In: FEBS Letters, Vol. 347, No. 2-3, 27.06.1994, p. 215-220.Research output: Contribution to journal › Article
TY - JOUR
T1 - Alteration of the erythrocyte glutathione redox balance by N-acetylcysteine, captopril and exogenous glutathione
AU - Russell, Jim
AU - Spickett, Corinne M.
AU - Reglinski, John
AU - Smith, W. Ewen
AU - McMurray, John
AU - Abdullah, Ibrahim B.
PY - 1994/6/27
Y1 - 1994/6/27
N2 - The effect of the thiol containing compounds N-acetylcysteine and captopril on glutathione metabolism in human erythrocytes has been investigated non-invasively using 1H spin echo NMR. N-Acetylcysteine was found to increase the reduced form of glutathione while captopril increased the oxidized form, but no changes in the total glutathione concentration were observed. Incubation of the cells with buthionine sulphoximine to inhibit de novo glutathione synthesis did not affect the response. The results show that these compounds act by altering the redox balance of glutathione rather than by stimulating its synthesis, and that their mechanisms of action are different, and not simply due to the presence of the thiol group.
AB - The effect of the thiol containing compounds N-acetylcysteine and captopril on glutathione metabolism in human erythrocytes has been investigated non-invasively using 1H spin echo NMR. N-Acetylcysteine was found to increase the reduced form of glutathione while captopril increased the oxidized form, but no changes in the total glutathione concentration were observed. Incubation of the cells with buthionine sulphoximine to inhibit de novo glutathione synthesis did not affect the response. The results show that these compounds act by altering the redox balance of glutathione rather than by stimulating its synthesis, and that their mechanisms of action are different, and not simply due to the presence of the thiol group.
KW - NMR
KW - antioxidant defense
KW - glutathione
KW - N-acetylcysteine
KW - captopril
KW - human erythrocyte
KW - Pharmacy and materia medica
UR - https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2894%2900548-6
U2 - 10.1016/0014-5793(94)00548-6
DO - 10.1016/0014-5793(94)00548-6
M3 - Article
VL - 347
SP - 215
EP - 220
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -