Alteration of the erythrocyte glutathione redox balance by N-acetylcysteine, captopril and exogenous glutathione

Jim  Russell; Corinne M. Spickett; John  Reglinski; W. Ewen  Smith; John  McMurray; Ibrahim B.  Abdullah

doi:10.1016/0014-5793(94)00548-6

Alteration of the erythrocyte glutathione redox balance by N-acetylcysteine, captopril and exogenous glutathione

Jim Russell, Corinne M. Spickett, John Reglinski, W. Ewen Smith, John McMurray, Ibrahim B. Abdullah

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of the thiol containing compounds N-acetylcysteine and captopril on glutathione metabolism in human erythrocytes has been investigated non-invasively using 1H spin echo NMR. N-Acetylcysteine was found to increase the reduced form of glutathione while captopril increased the oxidized form, but no changes in the total glutathione concentration were observed. Incubation of the cells with buthionine sulphoximine to inhibit de novo glutathione synthesis did not affect the response. The results show that these compounds act by altering the redox balance of glutathione rather than by stimulating its synthesis, and that their mechanisms of action are different, and not simply due to the presence of the thiol group.

Original language	English
Pages (from-to)	215-220
Number of pages	6
Journal	FEBS Letters
Volume	347
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(94)00548-6
Publication status	Published - 27 Jun 1994

Keywords

NMR
antioxidant defense
glutathione
N-acetylcysteine
captopril
human erythrocyte
Pharmacy and materia medica

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10.1016/0014-5793(94)00548-6

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title = "Alteration of the erythrocyte glutathione redox balance by N-acetylcysteine, captopril and exogenous glutathione",

abstract = "The effect of the thiol containing compounds N-acetylcysteine and captopril on glutathione metabolism in human erythrocytes has been investigated non-invasively using 1H spin echo NMR. N-Acetylcysteine was found to increase the reduced form of glutathione while captopril increased the oxidized form, but no changes in the total glutathione concentration were observed. Incubation of the cells with buthionine sulphoximine to inhibit de novo glutathione synthesis did not affect the response. The results show that these compounds act by altering the redox balance of glutathione rather than by stimulating its synthesis, and that their mechanisms of action are different, and not simply due to the presence of the thiol group.",

keywords = "NMR, antioxidant defense, glutathione, N-acetylcysteine, captopril, human erythrocyte, Pharmacy and materia medica",

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T1 - Alteration of the erythrocyte glutathione redox balance by N-acetylcysteine, captopril and exogenous glutathione

AU - Russell, Jim

AU - Spickett, Corinne M.

AU - Reglinski, John

AU - Smith, W. Ewen

AU - McMurray, John

AU - Abdullah, Ibrahim B.

PY - 1994/6/27

Y1 - 1994/6/27

N2 - The effect of the thiol containing compounds N-acetylcysteine and captopril on glutathione metabolism in human erythrocytes has been investigated non-invasively using 1H spin echo NMR. N-Acetylcysteine was found to increase the reduced form of glutathione while captopril increased the oxidized form, but no changes in the total glutathione concentration were observed. Incubation of the cells with buthionine sulphoximine to inhibit de novo glutathione synthesis did not affect the response. The results show that these compounds act by altering the redox balance of glutathione rather than by stimulating its synthesis, and that their mechanisms of action are different, and not simply due to the presence of the thiol group.

AB - The effect of the thiol containing compounds N-acetylcysteine and captopril on glutathione metabolism in human erythrocytes has been investigated non-invasively using 1H spin echo NMR. N-Acetylcysteine was found to increase the reduced form of glutathione while captopril increased the oxidized form, but no changes in the total glutathione concentration were observed. Incubation of the cells with buthionine sulphoximine to inhibit de novo glutathione synthesis did not affect the response. The results show that these compounds act by altering the redox balance of glutathione rather than by stimulating its synthesis, and that their mechanisms of action are different, and not simply due to the presence of the thiol group.

KW - NMR

KW - antioxidant defense

KW - glutathione

KW - N-acetylcysteine

KW - captopril

KW - human erythrocyte

KW - Pharmacy and materia medica

UR - https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2894%2900548-6

U2 - 10.1016/0014-5793(94)00548-6

DO - 10.1016/0014-5793(94)00548-6

M3 - Article

VL - 347

SP - 215

EP - 220

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2-3

ER -

Alteration of the erythrocyte glutathione redox balance by N-acetylcysteine, captopril and exogenous glutathione

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Keywords

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