An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography

Christian George George Burton, Danny Axford, Andrew Michael John Edwards, Richard J Gildea, Robert H Morris, Michael I Newton, Allen M Orville, Mark Prince, Paul Topham, Peter Docker

Research output: Contribution to journalArticle

Abstract

This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalLab on a Chip
DOIs
Publication statusPublished - 1 Nov 2017

Bibliographical note

© Royal Society of Chemistry 2017

Funding: Strategic Award from the Wellcome Trust and the
Biotechnology and Biological Sciences Research Council
(grant 102593).

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    Burton, C. G. G., Axford, D., Edwards, A. M. J., Gildea, R. J., Morris, R. H., Newton, M. I., Orville, A. M., Prince, M., Topham, P., & Docker, P. (2017). An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography. Lab on a Chip, 1-7. https://doi.org/10.1039/C7LC00812K