This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.
Bibliographical note© Royal Society of Chemistry 2017
Funding: Strategic Award from the Wellcome Trust and the
Biotechnology and Biological Sciences Research Council
Burton, C. G. G., Axford, D., Edwards, A. M. J., Gildea, R. J., Morris, R. H., Newton, M. I., Orville, A. M., Prince, M., Topham, P., & Docker, P. (2017). An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography. Lab on a Chip, 1-7. https://doi.org/10.1039/C7LC00812K