Abstract
Original language | English |
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Pages (from-to) | 1-7 |
Number of pages | 7 |
Journal | Lab on a Chip |
DOIs | |
Publication status | Published - 1 Nov 2017 |
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Bibliographical note
© Royal Society of Chemistry 2017Funding: Strategic Award from the Wellcome Trust and the
Biotechnology and Biological Sciences Research Council
(grant 102593).
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An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography. / Burton, Christian George George; Axford, Danny; Edwards, Andrew Michael John; Gildea, Richard J; Morris, Robert H; Newton, Michael I; Orville, Allen M; Prince, Mark; Topham, Paul; Docker, Peter.
In: Lab on a Chip, 01.11.2017, p. 1-7.Research output: Contribution to journal › Article
TY - JOUR
T1 - An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography
AU - Burton, Christian George George
AU - Axford, Danny
AU - Edwards, Andrew Michael John
AU - Gildea, Richard J
AU - Morris, Robert H
AU - Newton, Michael I
AU - Orville, Allen M
AU - Prince, Mark
AU - Topham, Paul
AU - Docker, Peter
N1 - © Royal Society of Chemistry 2017 Funding: Strategic Award from the Wellcome Trust and the Biotechnology and Biological Sciences Research Council (grant 102593).
PY - 2017/11/1
Y1 - 2017/11/1
N2 - This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.
AB - This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.
U2 - 10.1039/C7LC00812K
DO - 10.1039/C7LC00812K
M3 - Article
SP - 1
EP - 7
JO - Lab on a Chip
JF - Lab on a Chip
SN - 1473-0197
ER -