An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography

Christian George George Burton, Danny Axford, Andrew Michael John Edwards, Richard J Gildea, Robert H Morris, Michael I Newton, Allen M Orville, Mark Prince, Paul Topham, Peter Docker

Research output: Contribution to journalArticle

Abstract

This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalLab on a Chip
DOIs
Publication statusPublished - 1 Nov 2017

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Goniometers
Crystallography
Acoustics
Synchrotrons
Temperature
Fluids
Data acquisition
Actuators
Diffraction
Proteins
Costs and Cost Analysis
Equipment and Supplies
Costs

Bibliographical note

© Royal Society of Chemistry 2017

Funding: Strategic Award from the Wellcome Trust and the
Biotechnology and Biological Sciences Research Council
(grant 102593).

Cite this

Burton, C. G. G., Axford, D., Edwards, A. M. J., Gildea, R. J., Morris, R. H., Newton, M. I., ... Docker, P. (2017). An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography. Lab on a Chip, 1-7. https://doi.org/10.1039/C7LC00812K
Burton, Christian George George ; Axford, Danny ; Edwards, Andrew Michael John ; Gildea, Richard J ; Morris, Robert H ; Newton, Michael I ; Orville, Allen M ; Prince, Mark ; Topham, Paul ; Docker, Peter. / An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography. In: Lab on a Chip. 2017 ; pp. 1-7.
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Burton, CGG, Axford, D, Edwards, AMJ, Gildea, RJ, Morris, RH, Newton, MI, Orville, AM, Prince, M, Topham, P & Docker, P 2017, 'An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography', Lab on a Chip, pp. 1-7. https://doi.org/10.1039/C7LC00812K

An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography. / Burton, Christian George George; Axford, Danny; Edwards, Andrew Michael John; Gildea, Richard J; Morris, Robert H; Newton, Michael I; Orville, Allen M; Prince, Mark; Topham, Paul; Docker, Peter.

In: Lab on a Chip, 01.11.2017, p. 1-7.

Research output: Contribution to journalArticle

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AU - Burton, Christian George George

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AU - Gildea, Richard J

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AU - Newton, Michael I

AU - Orville, Allen M

AU - Prince, Mark

AU - Topham, Paul

AU - Docker, Peter

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AB - This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

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Burton CGG, Axford D, Edwards AMJ, Gildea RJ, Morris RH, Newton MI et al. An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography. Lab on a Chip. 2017 Nov 1;1-7. https://doi.org/10.1039/C7LC00812K