An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography

Christian George George Burton; Danny Axford; Andrew Michael John Edwards; Richard J Gildea; Robert H Morris; Michael I Newton; Allen M Orville; Mark Prince; Paul Topham; Peter Docker

doi:10.1039/C7LC00812K

An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography

Christian George George Burton, Danny Axford, Andrew Michael John Edwards, Richard J Gildea, Robert H Morris, Michael I Newton, Allen M Orville, Mark Prince, Paul Topham, Peter Docker

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Abstract

This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

Original language	English
Pages (from-to)	4225-4230
Number of pages	7
Journal	Lab on a Chip
Volume	17
Issue number	24
Early online date	1 Nov 2017
DOIs	https://doi.org/10.1039/C7LC00812K
Publication status	Published - 21 Dec 2017

Bibliographical note

© Royal Society of Chemistry 2017

Funding: Strategic Award from the Wellcome Trust and the
Biotechnology and Biological Sciences Research Council
(grant 102593).

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10.1039/C7LC00812K

An Acoustic On-Chip Goniometer for Room Temperature
© Royal Society of Chemistry 2017
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abstract = "This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.",

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AU - Burton, Christian George George

AU - Axford, Danny

AU - Edwards, Andrew Michael John

AU - Gildea, Richard J

AU - Morris, Robert H

AU - Newton, Michael I

AU - Orville, Allen M

AU - Prince, Mark

AU - Topham, Paul

AU - Docker, Peter

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N2 - This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

AB - This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

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JF - Lab on a Chip

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ER -

An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography

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