An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles

Marcel K. Welleweerd; Tijmen Hageman; Marc Pichel; Dave van As; Hans Keizer; Jordi Hendrix; Mina M. Micheal; Islam S. M. Khalil; Alveena Mir; Nuriye Korkmaz; Robbert Kräwinkel; Daniel M. Chevrier; Damien Faivre; Alfred Fernandez-Castane; Daniel Pfeiffer; Leon Abelmann

doi:10.1063/5.0098008

An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles

Marcel K. Welleweerd, Tijmen Hageman, Marc Pichel, Dave van As, Hans Keizer, Jordi Hendrix, Mina M. Micheal, Islam S. M. Khalil, Alveena Mir, Nuriye Korkmaz, Robbert Kräwinkel, Daniel M. Chevrier, Damien Faivre, Alfred Fernandez-Castane, Daniel Pfeiffer, Leon Abelmann

Research output: Contribution to journal › Article › peer-review

Abstract

We present a spectrophotometer (optical density meter) combined with electromagnets dedicated to the analysis of suspensions of magnetotactic bacteria. The instrument can also be applied to suspensions of other magnetic cells and magnetic particles. We have ensured that our system, called MagOD, can be easily reproduced by providing the source of the 3D prints for the housing, electronic designs, circuit board layouts, and microcontroller software. We compare the performance of our system to existing adapted commercial spectrophotometers. In addition, we demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a function of their orientation with respect to the light path and their speed of reorientation after the field has been rotated by 90°. We continuously monitored the development of a culture of magnetotactic bacteria over a period of 5 days and measured the development of their velocity distribution over a period of one hour. Even though this dedicated spectrophotometer is relatively simple to construct and cost-effective, a range of magnetic field-dependent parameters can be extracted from suspensions of magnetotactic bacteria. Therefore, this instrument will help the magnetotactic research community to understand and apply this intriguing micro-organism.

Original language	English
Article number	094101
Pages (from-to)	094101
Number of pages	20
Journal	Review of scientific instruments
Volume	93
Issue number	9
DOIs	https://doi.org/10.1063/5.0098008
Publication status	Published - 16 Sept 2022

Bibliographical note

© 2022 Author(s). Published under an exclusive license by AIP Publishing. This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Marcel K. Welleweerd, Tijmen Hageman, Marc Pichel, Dave van As, Hans Keizer, Jordi Hendrix, Mina M. Micheal, Islam S. M. Khalil, Alveena Mir, Nuriye Korkmaz, Robbert Kräwinkel, Daniel M. Chevrier, Damien Faivre, Alfred Fernandez-Castane, Daniel Pfeiffer, and Leon Abelmann , "An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles", Review of Scientific Instruments 93, 094101 (2022) https://doi.org/10.1063/5.0098008 and may be found at https://aip.scitation.org/doi/10.1063/5.0098008.

Keywords

Instrumentation

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10.1063/5.0098008

Welleweerdetal_2022_VoR
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Welleweerd, M. K., Hageman, T., Pichel, M., van As, D., Keizer, H., Hendrix, J., Micheal, M. M., Khalil, I. S. M., Mir, A., Korkmaz, N., Kräwinkel, R., Chevrier, D. M., Faivre, D., Fernandez-Castane, A., Pfeiffer, D., & Abelmann, L. (2022). An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles. Review of scientific instruments, 93(9), 094101. Article 094101. https://doi.org/10.1063/5.0098008

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title = "An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles",

abstract = "We present a spectrophotometer (optical density meter) combined with electromagnets dedicated to the analysis of suspensions of magnetotactic bacteria. The instrument can also be applied to suspensions of other magnetic cells and magnetic particles. We have ensured that our system, called MagOD, can be easily reproduced by providing the source of the 3D prints for the housing, electronic designs, circuit board layouts, and microcontroller software. We compare the performance of our system to existing adapted commercial spectrophotometers. In addition, we demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a function of their orientation with respect to the light path and their speed of reorientation after the field has been rotated by 90°. We continuously monitored the development of a culture of magnetotactic bacteria over a period of 5 days and measured the development of their velocity distribution over a period of one hour. Even though this dedicated spectrophotometer is relatively simple to construct and cost-effective, a range of magnetic field-dependent parameters can be extracted from suspensions of magnetotactic bacteria. Therefore, this instrument will help the magnetotactic research community to understand and apply this intriguing micro-organism.",

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author = "Welleweerd, {Marcel K.} and Tijmen Hageman and Marc Pichel and {van As}, Dave and Hans Keizer and Jordi Hendrix and Micheal, {Mina M.} and Khalil, {Islam S. M.} and Alveena Mir and Nuriye Korkmaz and Robbert Kr{\"a}winkel and Chevrier, {Daniel M.} and Damien Faivre and Alfred Fernandez-Castane and Daniel Pfeiffer and Leon Abelmann",

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Welleweerd, MK, Hageman, T, Pichel, M, van As, D, Keizer, H, Hendrix, J, Micheal, MM, Khalil, ISM, Mir, A, Korkmaz, N, Kräwinkel, R, Chevrier, DM, Faivre, D, Fernandez-Castane, A, Pfeiffer, D & Abelmann, L 2022, 'An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles', Review of scientific instruments, vol. 93, no. 9, 094101, pp. 094101. https://doi.org/10.1063/5.0098008

TY - JOUR

T1 - An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles

AU - Welleweerd, Marcel K.

AU - Hageman, Tijmen

AU - Pichel, Marc

AU - van As, Dave

AU - Keizer, Hans

AU - Hendrix, Jordi

AU - Micheal, Mina M.

AU - Khalil, Islam S. M.

AU - Mir, Alveena

AU - Korkmaz, Nuriye

AU - Kräwinkel, Robbert

AU - Chevrier, Daniel M.

AU - Faivre, Damien

AU - Fernandez-Castane, Alfred

AU - Pfeiffer, Daniel

AU - Abelmann, Leon

N1 - © 2022 Author(s). Published under an exclusive license by AIP Publishing. This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Marcel K. Welleweerd, Tijmen Hageman, Marc Pichel, Dave van As, Hans Keizer, Jordi Hendrix, Mina M. Micheal, Islam S. M. Khalil, Alveena Mir, Nuriye Korkmaz, Robbert Kräwinkel, Daniel M. Chevrier, Damien Faivre, Alfred Fernandez-Castane, Daniel Pfeiffer, and Leon Abelmann , "An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles", Review of Scientific Instruments 93, 094101 (2022) https://doi.org/10.1063/5.0098008 and may be found at https://aip.scitation.org/doi/10.1063/5.0098008.

PY - 2022/9/16

Y1 - 2022/9/16

N2 - We present a spectrophotometer (optical density meter) combined with electromagnets dedicated to the analysis of suspensions of magnetotactic bacteria. The instrument can also be applied to suspensions of other magnetic cells and magnetic particles. We have ensured that our system, called MagOD, can be easily reproduced by providing the source of the 3D prints for the housing, electronic designs, circuit board layouts, and microcontroller software. We compare the performance of our system to existing adapted commercial spectrophotometers. In addition, we demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a function of their orientation with respect to the light path and their speed of reorientation after the field has been rotated by 90°. We continuously monitored the development of a culture of magnetotactic bacteria over a period of 5 days and measured the development of their velocity distribution over a period of one hour. Even though this dedicated spectrophotometer is relatively simple to construct and cost-effective, a range of magnetic field-dependent parameters can be extracted from suspensions of magnetotactic bacteria. Therefore, this instrument will help the magnetotactic research community to understand and apply this intriguing micro-organism.

AB - We present a spectrophotometer (optical density meter) combined with electromagnets dedicated to the analysis of suspensions of magnetotactic bacteria. The instrument can also be applied to suspensions of other magnetic cells and magnetic particles. We have ensured that our system, called MagOD, can be easily reproduced by providing the source of the 3D prints for the housing, electronic designs, circuit board layouts, and microcontroller software. We compare the performance of our system to existing adapted commercial spectrophotometers. In addition, we demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a function of their orientation with respect to the light path and their speed of reorientation after the field has been rotated by 90°. We continuously monitored the development of a culture of magnetotactic bacteria over a period of 5 days and measured the development of their velocity distribution over a period of one hour. Even though this dedicated spectrophotometer is relatively simple to construct and cost-effective, a range of magnetic field-dependent parameters can be extracted from suspensions of magnetotactic bacteria. Therefore, this instrument will help the magnetotactic research community to understand and apply this intriguing micro-organism.

KW - Instrumentation

UR - https://aip.scitation.org/doi/10.1063/5.0098008

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DO - 10.1063/5.0098008

M3 - Article

SN - 0034-6748

VL - 93

SP - 094101

JO - Review of scientific instruments

JF - Review of scientific instruments

IS - 9

M1 - 094101

ER -

An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles

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