Analysis of oxidized and chlorinated lipids by mass spectrometry and relevance to signalling

Corinne M. Spickett, Norsyahida M. Fauzi

Research output: Contribution to journalArticle

Abstract

Oxidized and chlorinated phospholipids are generated under inflammatory conditions and are increasingly understood to play important roles in diseases involving oxidative stress. MS is a sensitive and informative technique for monitoring phospholipid oxidation that can provide structural information and simultaneously detect a wide variety of oxidation products, including chain-shortened and -chlorinated phospholipids. MSn technologies involve fragmentation of the compounds to yield diagnostic fragment ions and thus assist in identification. Advanced methods such as neutral loss and precursor ion scanning can facilitate the analysis of specific oxidation products in complex biological samples. This is essential for determining the contributions of different phospholipid oxidation products in disease. While many pro-inflammatory signalling effects of oxPLs (oxidized phospholipids) have been reported, it has more recently become clear that they can also have anti-inflammatory effects in conditions such as infection and endotoxaemia. In contrast with free radical-generated oxPLs, the signalling effects of chlorinated lipids are much less well understood, but they appear to demonstrate mainly pro-inflammatory effects. Specific analysis of oxidized and chlorinated lipids and the determination of their molecular effects are crucial to understanding their role in disease pathology.
LanguageEnglish
Pages1233-1239
Number of pages7
JournalBiochemical Society Transactions
Volume39
Issue number5
DOIs
Publication statusPublished - Oct 2011

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Mass spectrometry
Mass Spectrometry
Phospholipids
Lipids
Oxidation
Ions
Endotoxemia
Oxidative stress
Pathology
Free Radicals
Oxidative Stress
Anti-Inflammatory Agents
Technology
Scanning
Monitoring
Infection

Keywords

  • halogenation
  • lipids
  • mass spectrometry
  • oxidation-reduction
  • phospholipids
  • signal transduction

Cite this

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Analysis of oxidized and chlorinated lipids by mass spectrometry and relevance to signalling. / Spickett, Corinne M.; Fauzi, Norsyahida M.

In: Biochemical Society Transactions, Vol. 39, No. 5, 10.2011, p. 1233-1239.

Research output: Contribution to journalArticle

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