Analysis of protein carbonylation - pitfalls and promise in commonly used methods

A. Rogowska-Wrzesinska, K. Wojdyla, O. Nedić, C.P. Baron, H.R. Griffiths

Research output: Contribution to journalArticle

Abstract

Abstract Oxidation of proteins has received a lot of attention in the last decades due to the fact that they have been shown to accumulate and to be implicated in the progression and the patho-physiology of several diseases such as Alzheimer, coronary heart diseases, etc. This has also resulted in the fact that research scientist became more eager to be able to measure accurately the level of oxidized protein in biological materials, and to determine the precise site of the oxidative attack on the protein, in order to get insights into the molecular mechanisms involved in the progression of diseases. Several methods for measuring protein carbonylation have been implemented in different laboratories around the world. However, to date no methods prevail as the most accurate, reliable and robust. The present paper aims at giving an overview of the common methods used to determine protein carbonylation in biological material as well as to highlight the limitations and the potential. The ultimate goal is to give quick tips for a rapid decision making when a method has to be selected and taking into consideration the advantage and drawback of the methods.
LanguageEnglish
Pages1145-1162
Number of pages18
JournalFree Radical Research
Volume48
Issue number10
Early online date29 Jul 2014
DOIs
Publication statusPublished - 2014

Fingerprint

Protein Carbonylation
Carbonylation
Proteins
Biological materials
Physiology
Coronary Disease
Disease Progression
Decision Making
Decision making
Oxidation
Research

Bibliographical note

Copyright © 2014, Informa Healthcare

Keywords

  • carbonylation
  • standardization
  • mass spectrometry
  • derivatization
  • immunoaffinity

Cite this

Rogowska-Wrzesinska, A., Wojdyla, K., Nedić, O., Baron, C. P., & Griffiths, H. R. (2014). Analysis of protein carbonylation - pitfalls and promise in commonly used methods. Free Radical Research, 48(10), 1145-1162. https://doi.org/10.3109/10715762.2014.944868
Rogowska-Wrzesinska, A. ; Wojdyla, K. ; Nedić, O. ; Baron, C.P. ; Griffiths, H.R. / Analysis of protein carbonylation - pitfalls and promise in commonly used methods. In: Free Radical Research. 2014 ; Vol. 48, No. 10. pp. 1145-1162.
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Rogowska-Wrzesinska, A, Wojdyla, K, Nedić, O, Baron, CP & Griffiths, HR 2014, 'Analysis of protein carbonylation - pitfalls and promise in commonly used methods' Free Radical Research, vol. 48, no. 10, pp. 1145-1162. https://doi.org/10.3109/10715762.2014.944868

Analysis of protein carbonylation - pitfalls and promise in commonly used methods. / Rogowska-Wrzesinska, A.; Wojdyla, K.; Nedić, O.; Baron, C.P.; Griffiths, H.R.

In: Free Radical Research, Vol. 48, No. 10, 2014, p. 1145-1162.

Research output: Contribution to journalArticle

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AU - Rogowska-Wrzesinska, A.

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AU - Griffiths, H.R.

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AB - Abstract Oxidation of proteins has received a lot of attention in the last decades due to the fact that they have been shown to accumulate and to be implicated in the progression and the patho-physiology of several diseases such as Alzheimer, coronary heart diseases, etc. This has also resulted in the fact that research scientist became more eager to be able to measure accurately the level of oxidized protein in biological materials, and to determine the precise site of the oxidative attack on the protein, in order to get insights into the molecular mechanisms involved in the progression of diseases. Several methods for measuring protein carbonylation have been implemented in different laboratories around the world. However, to date no methods prevail as the most accurate, reliable and robust. The present paper aims at giving an overview of the common methods used to determine protein carbonylation in biological material as well as to highlight the limitations and the potential. The ultimate goal is to give quick tips for a rapid decision making when a method has to be selected and taking into consideration the advantage and drawback of the methods.

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