Analysis of secretory, immunostaining and clinical characteristics of human "functionless" pituitary adenomas: transdifferentiation or gonadotropinomas?

Ana L. de Oliveira Machado, Eric F. Adams, W. Schott, P. Nomikos, R. Fahlbusch, M. Buchfelder*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

In this study, the central technique of in vitro culture has been used to further investigate whether LH/FSH-expressing, but clinically "functionless" pituitary adenomas are gonadotropinomas or whether their hormone secretion is due to transdifferentiation events. 664 "functionless" pituitary adenomas were examined for hormone secretion by in vitro culture and for hormone content by immunostaining. The results were correlated with the clinical findings. 40% of the tumours (n = 263) secreted at least one of the gonadotropins alone, 8% (n = 53) exhibited various patterns of anterior pituitary hormones, whilst the remaining 52% of tumours were not associated with any hormone. In the secretory tumours, immunostaining revealed only a few scattered hormone-containing cells (5 to 15%). Mild hyperprolactinaemia was observed in some cases, presumably because of pressure effects of the tumours. The majority of the patients suffered clear cut hypopituitarism (p < 0.05). Pre-operatively, gonadotropin hypersecretion was observed in 3 cases, but only one of these secreted hormones in culture. Interestingly, a higher proportion of tumours removed from patients with hypopituitarism showed secretory activity in vitro than those tumours removed from patients showing no hormonal dysfunction or hyperprolactinaemia. We conclude that the term "gonadotropinoma" to describe functionless pituitary tumours associated with LH and/or FSH secretion is a misnomer, because the presence of LH and/or FSH confirmed by in vitro methods in the present series is a result of only a few scattered cells. We suggest that primary pituitary tumour cells differentiate into a secretory type (transdifferentiation), possibly in response to altered serum hormone levels such as decreased steroids. Further work is required to identify the factors which trigger the altered cells' characteristics. © J. A. Barth Verlag in Georg Thieme Verlag KG.

Original languageEnglish
Pages (from-to)344-349
Number of pages6
JournalExperimental and Clinical Endocrinology and Diabetes
Volume113
Issue number6
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Pituitary Neoplasms
Hormones
Neoplasms
Hypopituitarism
Hyperprolactinemia
Gonadotropins
Anterior Pituitary Hormones
Steroids
Pressure
In Vitro Techniques
Serum

Keywords

  • gonadotrophinoma transdifferentiation
  • hypopituitarism
  • in vitro
  • pituitary adenoma

Cite this

de Oliveira Machado, Ana L. ; Adams, Eric F. ; Schott, W. ; Nomikos, P. ; Fahlbusch, R. ; Buchfelder, M. / Analysis of secretory, immunostaining and clinical characteristics of human "functionless" pituitary adenomas : transdifferentiation or gonadotropinomas?. In: Experimental and Clinical Endocrinology and Diabetes . 2005 ; Vol. 113, No. 6. pp. 344-349.
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Analysis of secretory, immunostaining and clinical characteristics of human "functionless" pituitary adenomas : transdifferentiation or gonadotropinomas? / de Oliveira Machado, Ana L.; Adams, Eric F.; Schott, W.; Nomikos, P.; Fahlbusch, R.; Buchfelder, M.

In: Experimental and Clinical Endocrinology and Diabetes , Vol. 113, No. 6, 06.2005, p. 344-349.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of secretory, immunostaining and clinical characteristics of human "functionless" pituitary adenomas

T2 - transdifferentiation or gonadotropinomas?

AU - de Oliveira Machado, Ana L.

AU - Adams, Eric F.

AU - Schott, W.

AU - Nomikos, P.

AU - Fahlbusch, R.

AU - Buchfelder, M.

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AB - In this study, the central technique of in vitro culture has been used to further investigate whether LH/FSH-expressing, but clinically "functionless" pituitary adenomas are gonadotropinomas or whether their hormone secretion is due to transdifferentiation events. 664 "functionless" pituitary adenomas were examined for hormone secretion by in vitro culture and for hormone content by immunostaining. The results were correlated with the clinical findings. 40% of the tumours (n = 263) secreted at least one of the gonadotropins alone, 8% (n = 53) exhibited various patterns of anterior pituitary hormones, whilst the remaining 52% of tumours were not associated with any hormone. In the secretory tumours, immunostaining revealed only a few scattered hormone-containing cells (5 to 15%). Mild hyperprolactinaemia was observed in some cases, presumably because of pressure effects of the tumours. The majority of the patients suffered clear cut hypopituitarism (p < 0.05). Pre-operatively, gonadotropin hypersecretion was observed in 3 cases, but only one of these secreted hormones in culture. Interestingly, a higher proportion of tumours removed from patients with hypopituitarism showed secretory activity in vitro than those tumours removed from patients showing no hormonal dysfunction or hyperprolactinaemia. We conclude that the term "gonadotropinoma" to describe functionless pituitary tumours associated with LH and/or FSH secretion is a misnomer, because the presence of LH and/or FSH confirmed by in vitro methods in the present series is a result of only a few scattered cells. We suggest that primary pituitary tumour cells differentiate into a secretory type (transdifferentiation), possibly in response to altered serum hormone levels such as decreased steroids. Further work is required to identify the factors which trigger the altered cells' characteristics. © J. A. Barth Verlag in Georg Thieme Verlag KG.

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