Analysis of Transglutaminase-2 in macrophage function

Research output: Contribution to conferencePoster

Abstract

Programmed cell death, apoptosis, is a highly regulated process that removes damaged or unwanted cells in vivo and has significant immunological implications. Defective clearance of apoptotic cells by macrophages (professional phagocytes) is known to result in chronic inflammatory and autoimmune disease.
Transglutaminase-2 (TG2) is a Ca2+-dependent protein crosslinking enzyme known to play an important role in apoptotic cell clearance by macrophages through an ill-defined mechanism. Several studies have implicated TG2 in the apoptosis programme e.g. raised TG2 levels in cells undergoing apoptosis; increased cell death with down-regulation of TG2; up-regulation of TG2 in monocytes upon differentiation into macrophages. Defective clearance of apoptotic cells by TG2 null mice has been described though in this context the role of TG2 is yet to be elucidated.
Here we aim to characterise the role of TG2 in macrophage function with a focus on apoptotic cell clearance. THP-1 monocytes were induced to differentiate to macrophage-like cells by three different stimulants and were analysed for the presence of TG2. Macrophage-apoptotic cell interaction studies in the presence and absence of irreversible TG2 inhibitors resulted in significant inhibition of interaction indicating a possible role for TG2 in apoptotic cell clearance. TG2 was expressed at the macrophage cell surface and its association with ß3 integrin expression suggests the possible link between TG2 and ß3 integrins. Our current findings suggest that TG2 had got a crucial but yet to be defined role in apoptotic cell clearance.
LanguageEnglish
DOIs
Publication statusPublished - 3 Dec 2010
Event2010 British Society for Immunology Annual Congress - Liverpool, United Kingdom
Duration: 6 Dec 201010 Dec 2010
http://www.immunology.org/page.aspx?pid=1519

Conference

Conference2010 British Society for Immunology Annual Congress
CountryUnited Kingdom
CityLiverpool
Period6/12/1010/12/10
Internet address

Fingerprint

Macrophages
Apoptosis
transglutaminase 2
Integrins
Monocytes
Cell Death
Phagocytes
Cell Communication
Autoimmune Diseases
Chronic Disease
Up-Regulation
Down-Regulation
Enzymes

Bibliographical note

Published: Immunology (2010), Author Index. Immunology, 131: 191–204.

Cite this

Nadella, V., Griffin, M., & Devitt, A. (2010). Analysis of Transglutaminase-2 in macrophage function. Poster session presented at 2010 British Society for Immunology Annual Congress, Liverpool, United Kingdom. https://doi.org/10.1111/j.1365-2567.2010.03391.x
Nadella, Vinod ; Griffin, Martin ; Devitt, Andrew. / Analysis of Transglutaminase-2 in macrophage function. Poster session presented at 2010 British Society for Immunology Annual Congress, Liverpool, United Kingdom.
@conference{4cc66e9958ff42db8b8db4ffa155614d,
title = "Analysis of Transglutaminase-2 in macrophage function",
abstract = "Programmed cell death, apoptosis, is a highly regulated process that removes damaged or unwanted cells in vivo and has significant immunological implications. Defective clearance of apoptotic cells by macrophages (professional phagocytes) is known to result in chronic inflammatory and autoimmune disease.Transglutaminase-2 (TG2) is a Ca2+-dependent protein crosslinking enzyme known to play an important role in apoptotic cell clearance by macrophages through an ill-defined mechanism. Several studies have implicated TG2 in the apoptosis programme e.g. raised TG2 levels in cells undergoing apoptosis; increased cell death with down-regulation of TG2; up-regulation of TG2 in monocytes upon differentiation into macrophages. Defective clearance of apoptotic cells by TG2 null mice has been described though in this context the role of TG2 is yet to be elucidated.Here we aim to characterise the role of TG2 in macrophage function with a focus on apoptotic cell clearance. THP-1 monocytes were induced to differentiate to macrophage-like cells by three different stimulants and were analysed for the presence of TG2. Macrophage-apoptotic cell interaction studies in the presence and absence of irreversible TG2 inhibitors resulted in significant inhibition of interaction indicating a possible role for TG2 in apoptotic cell clearance. TG2 was expressed at the macrophage cell surface and its association with {\ss}3 integrin expression suggests the possible link between TG2 and {\ss}3 integrins. Our current findings suggest that TG2 had got a crucial but yet to be defined role in apoptotic cell clearance.",
author = "Vinod Nadella and Martin Griffin and Andrew Devitt",
note = "Published: Immunology (2010), Author Index. Immunology, 131: 191–204. ; 2010 British Society for Immunology Annual Congress ; Conference date: 06-12-2010 Through 10-12-2010",
year = "2010",
month = "12",
day = "3",
doi = "10.1111/j.1365-2567.2010.03391.x",
language = "English",
url = "http://www.immunology.org/page.aspx?pid=1519",

}

Nadella, V, Griffin, M & Devitt, A 2010, 'Analysis of Transglutaminase-2 in macrophage function' 2010 British Society for Immunology Annual Congress, Liverpool, United Kingdom, 6/12/10 - 10/12/10, . https://doi.org/10.1111/j.1365-2567.2010.03391.x

Analysis of Transglutaminase-2 in macrophage function. / Nadella, Vinod; Griffin, Martin; Devitt, Andrew.

2010. Poster session presented at 2010 British Society for Immunology Annual Congress, Liverpool, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Analysis of Transglutaminase-2 in macrophage function

AU - Nadella, Vinod

AU - Griffin, Martin

AU - Devitt, Andrew

N1 - Published: Immunology (2010), Author Index. Immunology, 131: 191–204.

PY - 2010/12/3

Y1 - 2010/12/3

N2 - Programmed cell death, apoptosis, is a highly regulated process that removes damaged or unwanted cells in vivo and has significant immunological implications. Defective clearance of apoptotic cells by macrophages (professional phagocytes) is known to result in chronic inflammatory and autoimmune disease.Transglutaminase-2 (TG2) is a Ca2+-dependent protein crosslinking enzyme known to play an important role in apoptotic cell clearance by macrophages through an ill-defined mechanism. Several studies have implicated TG2 in the apoptosis programme e.g. raised TG2 levels in cells undergoing apoptosis; increased cell death with down-regulation of TG2; up-regulation of TG2 in monocytes upon differentiation into macrophages. Defective clearance of apoptotic cells by TG2 null mice has been described though in this context the role of TG2 is yet to be elucidated.Here we aim to characterise the role of TG2 in macrophage function with a focus on apoptotic cell clearance. THP-1 monocytes were induced to differentiate to macrophage-like cells by three different stimulants and were analysed for the presence of TG2. Macrophage-apoptotic cell interaction studies in the presence and absence of irreversible TG2 inhibitors resulted in significant inhibition of interaction indicating a possible role for TG2 in apoptotic cell clearance. TG2 was expressed at the macrophage cell surface and its association with ß3 integrin expression suggests the possible link between TG2 and ß3 integrins. Our current findings suggest that TG2 had got a crucial but yet to be defined role in apoptotic cell clearance.

AB - Programmed cell death, apoptosis, is a highly regulated process that removes damaged or unwanted cells in vivo and has significant immunological implications. Defective clearance of apoptotic cells by macrophages (professional phagocytes) is known to result in chronic inflammatory and autoimmune disease.Transglutaminase-2 (TG2) is a Ca2+-dependent protein crosslinking enzyme known to play an important role in apoptotic cell clearance by macrophages through an ill-defined mechanism. Several studies have implicated TG2 in the apoptosis programme e.g. raised TG2 levels in cells undergoing apoptosis; increased cell death with down-regulation of TG2; up-regulation of TG2 in monocytes upon differentiation into macrophages. Defective clearance of apoptotic cells by TG2 null mice has been described though in this context the role of TG2 is yet to be elucidated.Here we aim to characterise the role of TG2 in macrophage function with a focus on apoptotic cell clearance. THP-1 monocytes were induced to differentiate to macrophage-like cells by three different stimulants and were analysed for the presence of TG2. Macrophage-apoptotic cell interaction studies in the presence and absence of irreversible TG2 inhibitors resulted in significant inhibition of interaction indicating a possible role for TG2 in apoptotic cell clearance. TG2 was expressed at the macrophage cell surface and its association with ß3 integrin expression suggests the possible link between TG2 and ß3 integrins. Our current findings suggest that TG2 had got a crucial but yet to be defined role in apoptotic cell clearance.

U2 - 10.1111/j.1365-2567.2010.03391.x

DO - 10.1111/j.1365-2567.2010.03391.x

M3 - Poster

ER -

Nadella V, Griffin M, Devitt A. Analysis of Transglutaminase-2 in macrophage function. 2010. Poster session presented at 2010 British Society for Immunology Annual Congress, Liverpool, United Kingdom. https://doi.org/10.1111/j.1365-2567.2010.03391.x