Analyzing recombinant protein production in Pichia pastoris with targeted proteomics

Roslyn M Bill, Annegret Ulke-Lemée, Stephanie P Cartwright, Rena Far, Jay Kim, Justin A MacDonald

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris system.

Original languageEnglish
Title of host publicationRecombinant Protein Production in Yeast
EditorsB. Gasser, D. Mattanovich
PublisherHumana Press
Pages187-209
Number of pages23
Volume1923
ISBN (Electronic)978-1-4939-9024-5
ISBN (Print)978-1-4939-9023-8
DOIs
Publication statusPublished - 9 Feb 2019

Publication series

NameMethods in Molecular Biology
Volume1923
ISSN (Print)1064-3745

Bibliographical note

This work was supported by grants from the Canadian Institutes of Health Research (CIHR, MOP#133543 to JAM), the European Commission Research Executive Agency (Marie Sklodowska-Curie International Incoming Fellowship to JAM and RMB), and the Biotechnology and Biological Sciences Research Council (International Partnering Award BB/P025927/1 to JAM and RMB).

Keywords

  • DAPK3
  • Mass spectrometry
  • MRM-MS
  • Multiple reaction monitoring
  • Recombinant protein production
  • Yeast
  • ZIPK

Fingerprint Dive into the research topics of 'Analyzing recombinant protein production in Pichia pastoris with targeted proteomics'. Together they form a unique fingerprint.

  • Cite this

    Bill, R. M., Ulke-Lemée, A., Cartwright, S. P., Far, R., Kim, J., & MacDonald, J. A. (2019). Analyzing recombinant protein production in Pichia pastoris with targeted proteomics. In B. Gasser, & D. Mattanovich (Eds.), Recombinant Protein Production in Yeast (Vol. 1923, pp. 187-209). (Methods in Molecular Biology; Vol. 1923). Humana Press. https://doi.org/10.1007/978-1-4939-9024-5_8