Analyzing recombinant protein production in Pichia pastoris with targeted proteomics

Roslyn M Bill; Annegret Ulke-Lemée; Stephanie P Cartwright; Rena Far; Jay Kim; Justin A MacDonald

doi:10.1007/978-1-4939-9024-5_8

Analyzing recombinant protein production in Pichia pastoris with targeted proteomics

Roslyn M Bill, Annegret Ulke-Lemée, Stephanie P Cartwright, Rena Far, Jay Kim, Justin A MacDonald

Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.

Research output: Chapter in Book/Published conference output › Chapter

1 Citation (Scopus)

Abstract

New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris system.

Original language	English
Title of host publication	Recombinant Protein Production in Yeast
Editors	B. Gasser, D. Mattanovich
Publisher	Humana Press
Pages	187-209
Number of pages	23
Volume	1923
ISBN (Electronic)	978-1-4939-9024-5
ISBN (Print)	978-1-4939-9023-8
DOIs	https://doi.org/10.1007/978-1-4939-9024-5_8
Publication status	Published - 9 Feb 2019

Publication series

Name	Methods in Molecular Biology
Volume	1923
ISSN (Print)	1064-3745

Bibliographical note

This work was supported by grants from the Canadian Institutes of Health Research (CIHR, MOP#133543 to JAM), the European Commission Research Executive Agency (Marie Sklodowska-Curie International Incoming Fellowship to JAM and RMB), and the Biotechnology and Biological Sciences Research Council (International Partnering Award BB/P025927/1 to JAM and RMB).

Funding

This work was supported by grants from the Canadian Institutes of Health Research (CIHR, MOP#133543 to JAM), the European Commission Research Executive Agency (Marie Sklodowska-Curie International Incoming Fellowship to JAM and RMB), and the Biotechnology and Biological Sciences Research Council (International Partnering Award BB/P025927/1 to JAM and RMB).

Keywords

DAPK3
Mass spectrometry
MRM-MS
Multiple reaction monitoring
Recombinant protein production
Yeast
ZIPK

Access to Document

10.1007/978-1-4939-9024-5_8

Cite this

Bill, R. M., Ulke-Lemée, A., Cartwright, S. P., Far, R., Kim, J., & MacDonald, J. A. (2019). Analyzing recombinant protein production in Pichia pastoris with targeted proteomics. In B. Gasser, & D. Mattanovich (Eds.), Recombinant Protein Production in Yeast (Vol. 1923, pp. 187-209). (Methods in Molecular Biology; Vol. 1923). Humana Press. https://doi.org/10.1007/978-1-4939-9024-5_8

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title = "Analyzing recombinant protein production in Pichia pastoris with targeted proteomics",

abstract = "New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris system.",

keywords = "DAPK3, Mass spectrometry, MRM-MS, Multiple reaction monitoring, Recombinant protein production, Yeast, ZIPK",

author = "Bill, {Roslyn M} and Annegret Ulke-Lem{\'e}e and Cartwright, {Stephanie P} and Rena Far and Jay Kim and MacDonald, {Justin A}",

note = "This work was supported by grants from the Canadian Institutes of Health Research (CIHR, MOP#133543 to JAM), the European Commission Research Executive Agency (Marie Sklodowska-Curie International Incoming Fellowship to JAM and RMB), and the Biotechnology and Biological Sciences Research Council (International Partnering Award BB/P025927/1 to JAM and RMB).",

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booktitle = "Recombinant Protein Production in Yeast",

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Analyzing recombinant protein production in Pichia pastoris with targeted proteomics. / Bill, Roslyn M; Ulke-Lemée, Annegret; Cartwright, Stephanie P et al.
Recombinant Protein Production in Yeast. ed. / B. Gasser; D. Mattanovich. Vol. 1923 Humana Press, 2019. p. 187-209 (Methods in Molecular Biology; Vol. 1923).

Research output: Chapter in Book/Published conference output › Chapter

TY - CHAP

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AU - Bill, Roslyn M

AU - Ulke-Lemée, Annegret

AU - Cartwright, Stephanie P

AU - Far, Rena

AU - Kim, Jay

AU - MacDonald, Justin A

N1 - This work was supported by grants from the Canadian Institutes of Health Research (CIHR, MOP#133543 to JAM), the European Commission Research Executive Agency (Marie Sklodowska-Curie International Incoming Fellowship to JAM and RMB), and the Biotechnology and Biological Sciences Research Council (International Partnering Award BB/P025927/1 to JAM and RMB).

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AB - New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris system.

KW - DAPK3

KW - Mass spectrometry

KW - MRM-MS

KW - Multiple reaction monitoring

KW - Recombinant protein production

KW - Yeast

KW - ZIPK

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SN - 978-1-4939-9023-8

VL - 1923

T3 - Methods in Molecular Biology

SP - 187

EP - 209

BT - Recombinant Protein Production in Yeast

A2 - Gasser, B.

A2 - Mattanovich, D.

PB - Humana Press

ER -

Analyzing recombinant protein production in Pichia pastoris with targeted proteomics

Abstract

Publication series

Bibliographical note

Funding

Keywords

Access to Document

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Cite this