Angiopoietin-2 confers Atheroprotection in apoE-/- mice by inhibiting LDL oxidation via nitric oxide

Asif Ahmed, Takeshi Fujisawa, Xi-Lin Niu, Shakil Ahmad, Bahjat Al-Ani, Kunal Chudasama, Allyah Abbas, Rahul Potluri, Vineet Bhandari, Clarence M. Findley, Gregory K.W. Lam, Jianhua Huang, Peter W. Hewett, Melissa J. Cudmore, Christopher D. Kontos

Research output: Contribution to journalArticle

Abstract

Atherosclerosis is promoted by a combination of hypercholesterolemia and vascular inflammation. The function of Angiopoietin (Ang)-2, a key regulator of angiogenesis, in the maintenance of large vessels is unknown. A single systemic administration of Ang-2 adenovirus (AdAng-2) to apoE-/- mice fed a Western diet significantly reduced atherosclerotic lesion size 8 40%) and oxidized LDL and macrophage content of the plaques. These beneficial effects were abolished by the inhibition of nitric oxide synthase (NOS). In endothelial cells, endothelial NOS activation per se inhibited LDL oxidation and Ang-2 stimulated NO release in a Tie2-dependent manner to decrease LDL oxidation. These findings demonstrate a novel atheroprotective role for Ang-2 when endothelial cell function is compromised and suggest that growth factors, which stimulate NO release without inducing inflammation, could offer atheroprotection.

LanguageEnglish
Pages1333-1336
Number of pages4
JournalCirculation Research
Volume104
Issue number12
DOIs
Publication statusPublished - 19 Jun 2009

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Angiopoietin-2
Apolipoproteins E
Nitric Oxide
Endothelial Cells
Inflammation
Nitric Oxide Synthase Type III
Hypercholesterolemia
Adenoviridae
Nitric Oxide Synthase
Blood Vessels
Atherosclerosis
Intercellular Signaling Peptides and Proteins
Macrophages
oxidized low density lipoprotein

Bibliographical note

© 2009 American Heart Association, Inc.

Keywords

  • angiopoietin-2
  • atherosclerosis
  • endothelial cells
  • LDL cholesterol
  • nitric oxide
  • nitric oxide synthases

Cite this

Ahmed, Asif ; Fujisawa, Takeshi ; Niu, Xi-Lin ; Ahmad, Shakil ; Al-Ani, Bahjat ; Chudasama, Kunal ; Abbas, Allyah ; Potluri, Rahul ; Bhandari, Vineet ; Findley, Clarence M. ; Lam, Gregory K.W. ; Huang, Jianhua ; Hewett, Peter W. ; Cudmore, Melissa J. ; Kontos, Christopher D. / Angiopoietin-2 confers Atheroprotection in apoE-/- mice by inhibiting LDL oxidation via nitric oxide. In: Circulation Research. 2009 ; Vol. 104, No. 12. pp. 1333-1336.
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Ahmed, A, Fujisawa, T, Niu, X-L, Ahmad, S, Al-Ani, B, Chudasama, K, Abbas, A, Potluri, R, Bhandari, V, Findley, CM, Lam, GKW, Huang, J, Hewett, PW, Cudmore, MJ & Kontos, CD 2009, 'Angiopoietin-2 confers Atheroprotection in apoE-/- mice by inhibiting LDL oxidation via nitric oxide' Circulation Research, vol. 104, no. 12, pp. 1333-1336. https://doi.org/10.1161/CIRCRESAHA.109.196154

Angiopoietin-2 confers Atheroprotection in apoE-/- mice by inhibiting LDL oxidation via nitric oxide. / Ahmed, Asif; Fujisawa, Takeshi; Niu, Xi-Lin; Ahmad, Shakil; Al-Ani, Bahjat; Chudasama, Kunal; Abbas, Allyah; Potluri, Rahul; Bhandari, Vineet; Findley, Clarence M.; Lam, Gregory K.W.; Huang, Jianhua; Hewett, Peter W.; Cudmore, Melissa J.; Kontos, Christopher D.

In: Circulation Research, Vol. 104, No. 12, 19.06.2009, p. 1333-1336.

Research output: Contribution to journalArticle

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T1 - Angiopoietin-2 confers Atheroprotection in apoE-/- mice by inhibiting LDL oxidation via nitric oxide

AU - Ahmed, Asif

AU - Fujisawa, Takeshi

AU - Niu, Xi-Lin

AU - Ahmad, Shakil

AU - Al-Ani, Bahjat

AU - Chudasama, Kunal

AU - Abbas, Allyah

AU - Potluri, Rahul

AU - Bhandari, Vineet

AU - Findley, Clarence M.

AU - Lam, Gregory K.W.

AU - Huang, Jianhua

AU - Hewett, Peter W.

AU - Cudmore, Melissa J.

AU - Kontos, Christopher D.

N1 - © 2009 American Heart Association, Inc.

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KW - atherosclerosis

KW - endothelial cells

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EP - 1336

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SN - 0009-7330

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ER -