Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

Sarah J. Routledge, Christopher J. Hewitt, Nagamani Bora, Roslyn M. Bill

Research output: Contribution to journalArticle

Abstract

Pichia pastoris is a widely-used host for recombinant protein production. Initial screening for both suitable clones and optimum culture conditions is typically carried out in multi-well plates. This is followed by up-scaling either to shake-flasks or continuously stirred tank bioreactors. A particular problem in these formats is foaming, which is commonly prevented by the addition of chemical antifoaming agents. Intriguingly, antifoams are often added without prior consideration of their effect on the yeast cells, the protein product or the influence on downstream processes such as protein purification. In this study we characterised, for the first time, the effects of five commonly-used antifoaming agents on the total amount of recombinant green fluorescent protein (GFP) secreted from shake-flask cultures of this industrially-relevant yeast.
Original languageEnglish
Article number17
Number of pages11
JournalMicrobial Cell Factories
Volume10
Issue number17
DOIs
Publication statusPublished - 22 Mar 2011

Bibliographical note

© 2011 Routledge et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • antifoaming agents
  • biomass
  • bioreactors
  • culture media
  • green fluorescent proteins
  • Pichia
  • polymers
  • propylene glycols
  • recombinant proteins

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