Approaches to Investigating the Protein Interactome of PTEN

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The tumor suppressor phosphatase and tensin homologue (PTEN) is a redox-sensitive dual specificity phosphatase with an essential role in the negative regulation of the PI3K-AKT signaling pathway, affecting metabolic and cell survival processes. PTEN is commonly mutated in cancer, and dysregulation in the metabolism of PIP3 is implicated in other diseases such as diabetes. PTEN interactors are responsible for some functional roles of PTEN beyond the negative regulation of the PI3K pathway and are thus of great importance in cell biology. Both high-data content proteomics-based approaches and low-data content PPI approaches have been used to investigate the interactome of PTEN and elucidate further functions of PTEN. While low-data content approaches rely on co-immunoprecipitation and Western blotting, and as such require previously generated hypotheses, high-data content approaches such as affinity pull-down proteomic assays or the yeast 2-hybrid system are hypothesis generating. This review provides an overview of the PTEN interactome, including redox effects, and critically appraises the methods and results of high-data content investigations into the global interactome of PTEN. The biological significance of findings from recent studies is discussed and illustrates the breadth of cellular functions of PTEN that can be discovered by these approaches.
Original languageEnglish
Pages (from-to)60–77
Number of pages18
JournalJournal of Proteome Research
Issue number1
Early online date19 Oct 2020
Publication statusPublished - 1 Jan 2021

Bibliographical note

This document is the Accepted Manuscript version of a Published Work that appeared in final form in J. Proteome Res, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see

Funding: This project has received funding from Aston University (UK)
and the European Union’s Horizon 2020 research and
innovation program under the Marie Sklowdowska-Curie
Grant Agreement No. 675132


  • Biochemistry
  • General Chemistry


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