Aptamer-based surface plasmon sensor for thrombin detection

Thomas D.P. Allsop, David Nagel, Ron Neal, Edward M. Davies, Chengbo Mou, Peter Bond, Saeed Rehman, Kyriacos Kalli, David J. Webb, Phil Calverhouse, Marco Mascini, Ian Bennion

Research output: Chapter in Book/Published conference outputConference publication


A series of surface plasmonic fibre devices were fabricated using multiple coatings deposited on a lapped section of a single mode fibre and post-fabrication UV laser irradiation processing with a phase mask, producing a surface relief grating structure. These devices showed high spectral sensitivity in the aqueous index regime ranging up to 4000 nm/RIU for wavelength and 800 dB/RIU for intensity. The devices were then coated with human thrombin binding aptamer. Several concentrations of thrombin in buffer solution were made, ranging from 1nM to 1µM. All the concentrations were detectable by the devices demonstrating that sub-nM concentrations may be monitored.
Original languageEnglish
Title of host publicationBiophotonics
Subtitle of host publicationphotonic solutions for better health care II
EditorsJürgen Popp, Wolfgangs Drexler, Valery V. Tuchin, Dennis L. Matthew
Number of pages6
ISBN (Print)978-0-8194-8188-7
Publication statusPublished - Apr 2010
EventBiophotonics: Photonic Solutions for Better Health Care - Brussels, Belgium
Duration: 12 Apr 201016 Apr 2010

Publication series

NameSPIE proceedings
ISSN (Print)0277-786X


ConferenceBiophotonics: Photonic Solutions for Better Health Care

Bibliographical note

Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151C (May 17, 2010)
Allsop, Thomas D.P.; Nagel, David; Neal, Ron; Davies, Edward M.; Mou, Chengbo; Bond, Peter; Rehman, Saeed; Kalli, Kyriacos; Webb, David J.; Calverhouse, Phil; Mascini, Marco; Bennion, Ian, "Aptamer-based surface plasmon sensor for thrombin detection", Biophotonics: photonic solutions for better health care II., ed. Jürgen Popp; Wolfgangs Drexler; Valery V. Tuchin; Dennis L. Matthew. Vol. 7715 SPIE, 2010. (SPIE proceedings).

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  • bio-sensing
  • thrombin
  • fibre optic sensors
  • surface plasmons


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