Aptamer-based surface plasmon sensor for thrombin detection

Thomas D.P. Allsop; David Nagel; Ron Neal; Edward M. Davies; Chengbo Mou; Peter Bond; Saeed Rehman; Kyriacos Kalli; David J. Webb; Phil Calverhouse; Marco Mascini; Ian Bennion

doi:10.1117/12.854334

Aptamer-based surface plasmon sensor for thrombin detection

Thomas D.P. Allsop, David Nagel, Ron Neal, Edward M. Davies, Chengbo Mou, Peter Bond, Saeed Rehman, Kyriacos Kalli, David J. Webb, Phil Calverhouse, Marco Mascini, Ian Bennion

Research output: Chapter in Book/Published conference output › Conference publication

Abstract

A series of surface plasmonic fibre devices were fabricated using multiple coatings deposited on a lapped section of a single mode fibre and post-fabrication UV laser irradiation processing with a phase mask, producing a surface relief grating structure. These devices showed high spectral sensitivity in the aqueous index regime ranging up to 4000 nm/RIU for wavelength and 800 dB/RIU for intensity. The devices were then coated with human thrombin binding aptamer. Several concentrations of thrombin in buffer solution were made, ranging from 1nM to 1µM. All the concentrations were detectable by the devices demonstrating that sub-nM concentrations may be monitored.

Original language	English
Title of host publication	Biophotonics
Subtitle of host publication	photonic solutions for better health care II
Editors	Jürgen Popp, Wolfgangs Drexler, Valery V. Tuchin, Dennis L. Matthew
Publisher	SPIE
Number of pages	6
Volume	7715
ISBN (Print)	978-0-8194-8188-7
DOIs	https://doi.org/10.1117/12.854334
Publication status	Published - Apr 2010
Event	Biophotonics: Photonic Solutions for Better Health Care - Brussels, Belgium Duration: 12 Apr 2010 → 16 Apr 2010

Publication series

Name	SPIE proceedings
Publisher	SPIE
Volume	7715
ISSN (Print)	0277-786X

Conference

Conference	Biophotonics: Photonic Solutions for Better Health Care
Country/Territory	Belgium
City	Brussels
Period	12/04/10 → 16/04/10

Bibliographical note

Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151C (May 17, 2010)
Allsop, Thomas D.P.; Nagel, David; Neal, Ron; Davies, Edward M.; Mou, Chengbo; Bond, Peter; Rehman, Saeed; Kalli, Kyriacos; Webb, David J.; Calverhouse, Phil; Mascini, Marco; Bennion, Ian, "Aptamer-based surface plasmon sensor for thrombin detection", Biophotonics: photonic solutions for better health care II., ed. Jürgen Popp; Wolfgangs Drexler; Valery V. Tuchin; Dennis L. Matthew. Vol. 7715 SPIE, 2010. (SPIE proceedings).

Copyright 2010 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

http://dx.doi.org/10.1117/12.854334

Keywords

bio-sensing
thrombin
fibre optic sensors
surface plasmons

Access to Document

10.1117/12.854334

Aptamer-based surface plasmon fibre sensor
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151C (May 17, 2010) Allsop, Thomas D.P.; Nagel, David; Neal, Ron; Davies, Edward M.; Mou, Chengbo; Bond, Peter; Rehman, Saeed; Kalli, Kyriacos; Webb, David J.; Calverhouse, Phil; Mascini, Marco; Bennion, Ian, "Aptamer-based surface plasmon sensor for thrombin detection", Biophotonics: photonic solutions for better health care II., ed. Jürgen Popp; Wolfgangs Drexler; Valery V. Tuchin; Dennis L. Matthew. Vol. 7715 SPIE, 2010. (SPIE proceedings). Copyright 2010 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.854334
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http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1345322

Cite this

Allsop, T. D. P., Nagel, D., Neal, R., Davies, E. M., Mou, C., Bond, P., Rehman, S., Kalli, K., Webb, D. J., Calverhouse, P., Mascini, M., & Bennion, I. (2010). Aptamer-based surface plasmon sensor for thrombin detection. In J. Popp, W. Drexler, V. V. Tuchin, & D. L. Matthew (Eds.), Biophotonics: photonic solutions for better health care II (Vol. 7715). (SPIE proceedings; Vol. 7715). SPIE. https://doi.org/10.1117/12.854334

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title = "Aptamer-based surface plasmon sensor for thrombin detection",

abstract = "A series of surface plasmonic fibre devices were fabricated using multiple coatings deposited on a lapped section of a single mode fibre and post-fabrication UV laser irradiation processing with a phase mask, producing a surface relief grating structure. These devices showed high spectral sensitivity in the aqueous index regime ranging up to 4000 nm/RIU for wavelength and 800 dB/RIU for intensity. The devices were then coated with human thrombin binding aptamer. Several concentrations of thrombin in buffer solution were made, ranging from 1nM to 1µM. All the concentrations were detectable by the devices demonstrating that sub-nM concentrations may be monitored.",

keywords = "bio-sensing, thrombin, fibre optic sensors, surface plasmons",

author = "Allsop, {Thomas D.P.} and David Nagel and Ron Neal and Davies, {Edward M.} and Chengbo Mou and Peter Bond and Saeed Rehman and Kyriacos Kalli and Webb, {David J.} and Phil Calverhouse and Marco Mascini and Ian Bennion",

note = "Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151C (May 17, 2010) Allsop, Thomas D.P.; Nagel, David; Neal, Ron; Davies, Edward M.; Mou, Chengbo; Bond, Peter; Rehman, Saeed; Kalli, Kyriacos; Webb, David J.; Calverhouse, Phil; Mascini, Marco; Bennion, Ian, {"}Aptamer-based surface plasmon sensor for thrombin detection{"}, Biophotonics: photonic solutions for better health care II., ed. J{\"u}rgen Popp; Wolfgangs Drexler; Valery V. Tuchin; Dennis L. Matthew. Vol. 7715 SPIE, 2010. (SPIE proceedings). Copyright 2010 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.854334; Biophotonics: Photonic Solutions for Better Health Care ; Conference date: 12-04-2010 Through 16-04-2010",

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doi = "10.1117/12.854334",

language = "English",

isbn = "978-0-8194-8188-7",

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Allsop, TDP, Nagel, D, Neal, R, Davies, EM, Mou, C, Bond, P, Rehman, S, Kalli, K, Webb, DJ, Calverhouse, P, Mascini, M & Bennion, I 2010, Aptamer-based surface plasmon sensor for thrombin detection. in J Popp, W Drexler, VV Tuchin & DL Matthew (eds), Biophotonics: photonic solutions for better health care II. vol. 7715, SPIE proceedings, vol. 7715, SPIE, Biophotonics: Photonic Solutions for Better Health Care, Brussels, Belgium, 12/04/10. https://doi.org/10.1117/12.854334

Aptamer-based surface plasmon sensor for thrombin detection. / Allsop, Thomas D.P.; Nagel, David; Neal, Ron et al.
Biophotonics: photonic solutions for better health care II. ed. / Jürgen Popp; Wolfgangs Drexler; Valery V. Tuchin; Dennis L. Matthew. Vol. 7715 SPIE, 2010. (SPIE proceedings; Vol. 7715).

Research output: Chapter in Book/Published conference output › Conference publication

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T1 - Aptamer-based surface plasmon sensor for thrombin detection

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AU - Nagel, David

AU - Neal, Ron

AU - Davies, Edward M.

AU - Mou, Chengbo

AU - Bond, Peter

AU - Rehman, Saeed

AU - Kalli, Kyriacos

AU - Webb, David J.

AU - Calverhouse, Phil

AU - Mascini, Marco

AU - Bennion, Ian

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KW - bio-sensing

KW - thrombin

KW - fibre optic sensors

KW - surface plasmons

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SN - 978-0-8194-8188-7

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T3 - SPIE proceedings

BT - Biophotonics

A2 - Popp, Jürgen

A2 - Drexler, Wolfgangs

A2 - Tuchin, Valery V.

A2 - Matthew, Dennis L.

PB - SPIE

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Y2 - 12 April 2010 through 16 April 2010

ER -

Aptamer-based surface plasmon sensor for thrombin detection

Abstract

Publication series

Conference

Bibliographical note

Keywords

Access to Document

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