@inbook{1edb61d899164eeabe05e5411b774aa5,
title = "Assays for transcription factor activity",
abstract = "Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.",
keywords = "Biological Assay/methods, Cyclic AMP Receptor Protein/metabolism, DNA Footprinting, DNA-Directed RNA Polymerases/metabolism, Escherichia coli/enzymology, Plasmids/genetics, Potassium Permanganate, Promoter Regions, Genetic/genetics, RNA, Messenger/genetics, Transcription Factors/metabolism, Transcription, Genetic, Transcriptional Activation/genetics",
author = "Douglas Browning and Nigel Savery and Annie Kolb and Stephen Busby",
year = "2009",
month = mar,
doi = "10.1007/978-1-60327-015-1_23",
language = "English",
isbn = "978-1-60327-014-4",
volume = "543",
series = "Methods in molecular biology",
publisher = "Humana Press",
pages = "369--387",
editor = "B. Leblanc and T. Moss",
booktitle = "DNA-Protein Interactions",
address = "United States",
}