Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification

Martin Kögler, Yury V. Ryabchikov, Sanna Uusitalo, Alexey Popov, Gleb Tselikov, Anna Liisa Välimaa, Ahmed Al-Kattan, Jussi Hiltunen, Riitta Laitinen, Peter Neubauer, Igor Meglinski, Andrei V. Kabashin*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials. (Figure presented.).

Original languageEnglish
Article numbere201700225
JournalJournal of Biophotonics
Volume11
Issue number7
Early online date10 Apr 2018
DOIs
Publication statusPublished - 8 Jul 2018

Fingerprint

Raman Spectrum Analysis
Nanostructures
Nanostructured materials
Nanoparticles
bacteria
Raman scattering
Bacteria
Lasers
Raman spectra
nanoparticles
probes
lasers
Listeria
Food safety
Molecules
augmentation
Metal Nanoparticles
biocompatibility
Biological systems
Precious metals

Keywords

  • bacteria detection
  • laser ablation in liquids
  • Raman spectroscopy
  • SERS
  • ultrapure laser-synthesized Au nanoparticles

Cite this

Kögler, M., Ryabchikov, Y. V., Uusitalo, S., Popov, A., Tselikov, G., Välimaa, A. L., ... Kabashin, A. V. (2018). Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification. Journal of Biophotonics, 11(7), [e201700225]. https://doi.org/10.1002/jbio.201700225
Kögler, Martin ; Ryabchikov, Yury V. ; Uusitalo, Sanna ; Popov, Alexey ; Tselikov, Gleb ; Välimaa, Anna Liisa ; Al-Kattan, Ahmed ; Hiltunen, Jussi ; Laitinen, Riitta ; Neubauer, Peter ; Meglinski, Igor ; Kabashin, Andrei V. / Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification. In: Journal of Biophotonics. 2018 ; Vol. 11, No. 7.
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abstract = "The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials. (Figure presented.).",
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Kögler, M, Ryabchikov, YV, Uusitalo, S, Popov, A, Tselikov, G, Välimaa, AL, Al-Kattan, A, Hiltunen, J, Laitinen, R, Neubauer, P, Meglinski, I & Kabashin, AV 2018, 'Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification', Journal of Biophotonics, vol. 11, no. 7, e201700225. https://doi.org/10.1002/jbio.201700225

Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification. / Kögler, Martin; Ryabchikov, Yury V.; Uusitalo, Sanna; Popov, Alexey; Tselikov, Gleb; Välimaa, Anna Liisa; Al-Kattan, Ahmed; Hiltunen, Jussi; Laitinen, Riitta; Neubauer, Peter; Meglinski, Igor; Kabashin, Andrei V.

In: Journal of Biophotonics, Vol. 11, No. 7, e201700225, 08.07.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification

AU - Kögler, Martin

AU - Ryabchikov, Yury V.

AU - Uusitalo, Sanna

AU - Popov, Alexey

AU - Tselikov, Gleb

AU - Välimaa, Anna Liisa

AU - Al-Kattan, Ahmed

AU - Hiltunen, Jussi

AU - Laitinen, Riitta

AU - Neubauer, Peter

AU - Meglinski, Igor

AU - Kabashin, Andrei V.

PY - 2018/7/8

Y1 - 2018/7/8

N2 - The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials. (Figure presented.).

AB - The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials. (Figure presented.).

KW - bacteria detection

KW - laser ablation in liquids

KW - Raman spectroscopy

KW - SERS

KW - ultrapure laser-synthesized Au nanoparticles

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Kögler M, Ryabchikov YV, Uusitalo S, Popov A, Tselikov G, Välimaa AL et al. Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification. Journal of Biophotonics. 2018 Jul 8;11(7). e201700225. https://doi.org/10.1002/jbio.201700225