TY - JOUR
T1 - Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification
AU - Kögler, Martin
AU - Ryabchikov, Yury V.
AU - Uusitalo, Sanna
AU - Popov, Alexey
AU - Tselikov, Gleb
AU - Välimaa, Anna Liisa
AU - Al-Kattan, Ahmed
AU - Hiltunen, Jussi
AU - Laitinen, Riitta
AU - Neubauer, Peter
AU - Meglinski, Igor
AU - Kabashin, Andrei V.
PY - 2018/7/8
Y1 - 2018/7/8
N2 - The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials. (Figure presented.).
AB - The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials. (Figure presented.).
KW - bacteria detection
KW - laser ablation in liquids
KW - Raman spectroscopy
KW - SERS
KW - ultrapure laser-synthesized Au nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85044961771&partnerID=8YFLogxK
UR - https://onlinelibrary.wiley.com/doi/full/10.1002/jbio.201700225
U2 - 10.1002/jbio.201700225
DO - 10.1002/jbio.201700225
M3 - Article
AN - SCOPUS:85044961771
SN - 1864-063X
VL - 11
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 7
M1 - e201700225
ER -