Beyond defoaming

the effects of antifoams on bioprocesses productivity

Research output: Contribution to journalArticle

Abstract

Antifoams are often added to bioprocesses with little knowledge of their impact on the cells or product. However, it is known that certain antifoams can affect the growth rates of both prokaryotic and eukaryotic organisms in addition to changing surface properties such as lipid content, resulting in changes to permeability. This in turn can be beneficial to a recombinant protein production system for soluble proteins, as has been demonstrated by increased secretion of a-amylase and GFP, or achievement of greater yields of protein due to increased biomass. However, in some cases, certain concentrations of antifoams appear to have a detrimental effect upon cells and protein production, and the effects vary depending upon the protein being expressed. These findings emphasise the importance of optimising and understanding antifoam addition to bioprocesses.
Original languageEnglish
Article numbere201210014
Number of pages7
JournalComputational and Structural Biotechnology Journal
Volume3
Issue number4
DOIs
Publication statusPublished - Oct 2012

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Productivity
Proteins
Efficiency
Recombinant proteins
Amylases
Surface Properties
Recombinant Proteins
Biomass
Lipids
Surface properties
Permeability
Growth

Bibliographical note

© 2012 Routledge.
Licensee: Computational and Structural Biotechnology Journal.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly cited.

Keywords

  • recombinant protein
  • P. pastoris
  • kLa
  • oxygen transfer
  • optimization

Cite this

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Beyond defoaming : the effects of antifoams on bioprocesses productivity. / Routledge, Sarah J.

In: Computational and Structural Biotechnology Journal, Vol. 3, No. 4, e201210014, 10.2012.

Research output: Contribution to journalArticle

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