Abstract
Antifoams are often added to bioprocesses with little knowledge of their impact on the cells or product. However, it is known that certain antifoams can affect the growth rates of both prokaryotic and eukaryotic organisms in addition to changing surface properties such as lipid content, resulting in changes to permeability. This in turn can be beneficial to a recombinant protein production system for soluble proteins, as has been demonstrated by increased secretion of a-amylase and GFP, or achievement of greater yields of protein due to increased biomass. However, in some cases, certain concentrations of antifoams appear to have a detrimental effect upon cells and protein production, and the effects vary depending upon the protein being expressed. These findings emphasise the importance of optimising and understanding antifoam addition to bioprocesses.
| Original language | English |
|---|---|
| Article number | e201210014 |
| Number of pages | 7 |
| Journal | Computational and Structural Biotechnology Journal |
| Volume | 3 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Oct 2012 |
Bibliographical note
© 2012 Routledge.Licensee: Computational and Structural Biotechnology Journal.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly cited.
Keywords
- recombinant protein
- P. pastoris
- kLa
- oxygen transfer
- optimization