Beyond life: Exploring hemodynamic patterns in postmortem mice brains

Anton Sdobnov; Vassiliy Tsytsarev; Gennadi Piavchenko; Alexander Bykov; Igor Meglinski

doi:10.1002/jbio.202400017

Beyond life: Exploring hemodynamic patterns in postmortem mice brains

Anton Sdobnov, Vassiliy Tsytsarev, Gennadi Piavchenko, Alexander Bykov, Igor Meglinski^*

^*Corresponding author for this work

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Abstract

We utilize Laser Speckle Contrast Imaging (LSCI) for visualizing cerebral blood flow in mice during and post-cardiac arrest. Analyzing LSCI images, we noted temporal blood flow variations across the brain surface for hours postmortem. Fast Fourier Transform (FFT) analysis depicted blood flow and microcirculation decay post-death. Continuous Wavelet Transform (CWT) identified potential cerebral hemodynamic synchronization patterns. Additionally, non-negative matrix factorization (NMF) with four components segmented LSCI images, revealing structural subcomponent alterations over time. This integrated approach of LSCI, FFT, CWT, and NMF offers a comprehensive tool for studying cerebral blood flow dynamics, metaphorically capturing the ‘end of the tunnel’ experience. Results showed primary postmortem hemodynamic activity in the olfactory bulbs, followed by blood microflow relocations between somatosensory and visual cortical regions via the superior sagittal sinus. This method opens new avenues for exploring these phenomena, potentially linking neuroscientific insights with mysteries surrounding consciousness and perception at life's end.

Original language	English
Article number	e202400017
Number of pages	11
Journal	Journal of Biophotonics
Volume	17
Issue number	7
Early online date	7 May 2024
DOIs	https://doi.org/10.1002/jbio.202400017
Publication status	Published - Jul 2024

Bibliographical note

Copyright © 2024 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Data Access Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Funding

Current study supported by COST Action CA21159 - Understanding interaction light - biological surfaces: possibility for new electronic materials and devices (PhoBioS), the European Union's Horizon 2020 research and innovation programme under grant agreement no. 863214—NEUROPA project and by the (UK), and the ‘Perfect Match’ Public Engagement grant. Authors also acknowledge partial support provided by the Russian Science Foundation—project no. 22-65-00096.

Funders	Funder number
European Cooperation in Science and Technology
Horizon 2020	863214
Horizon 2020
Russian Science Foundation	22‐65‐00096
Russian Science Foundation

Keywords

blood flow
brain microcirculation
continuous wavelet transform
laser speckle contrast
non-negative matrix factorization
postmortem

Access to Document

10.1002/jbio.202400017Licence: CC BY 4.0

Journal of Biophotonics - 2024 - Sdobnov - Beyond life Exploring hemodynamic patterns in postmortem mice brains
Copyright © 2024 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.89 MBLicence: CC BY 4.0

Cite this

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title = "Beyond life: Exploring hemodynamic patterns in postmortem mice brains",

abstract = "We utilize Laser Speckle Contrast Imaging (LSCI) for visualizing cerebral blood flow in mice during and post-cardiac arrest. Analyzing LSCI images, we noted temporal blood flow variations across the brain surface for hours postmortem. Fast Fourier Transform (FFT) analysis depicted blood flow and microcirculation decay post-death. Continuous Wavelet Transform (CWT) identified potential cerebral hemodynamic synchronization patterns. Additionally, non-negative matrix factorization (NMF) with four components segmented LSCI images, revealing structural subcomponent alterations over time. This integrated approach of LSCI, FFT, CWT, and NMF offers a comprehensive tool for studying cerebral blood flow dynamics, metaphorically capturing the {\textquoteleft}end of the tunnel{\textquoteright} experience. Results showed primary postmortem hemodynamic activity in the olfactory bulbs, followed by blood microflow relocations between somatosensory and visual cortical regions via the superior sagittal sinus. This method opens new avenues for exploring these phenomena, potentially linking neuroscientific insights with mysteries surrounding consciousness and perception at life's end.",

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Beyond life: Exploring hemodynamic patterns in postmortem mice brains

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Keywords

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