Biocomposites of non-crosslinked natural and synthetic polymers

A.G.A. Coombes, E. Verderio, B. Shaw, Xiaoling Li, Martin Griffin, S. Downes

Research output: Contribution to journalArticle

Abstract

Biocomposite films comprising a non-crosslinked, natural polymer (collagen) and a synthetic polymer, poly(var epsilon-caprolactone) (PCL), have been produced by impregnation of lyophilised collagen mats with a solution of PCL in dichloromethane followed by solvent evaporation. This approach avoids the toxicity problems associated with chemical crosslinking. Distinct changes in film morphology, from continuous surface coating to open porous format, were achieved by variation of processing parameters such as collagen:PCL ratio and the weight of the starting lyophilised collagen mat. Collagenase digestion indicated that the collagen content of 1:4 and 1:8 collagen:PCL biocomposites was almost totally accessible for enzymatic digestion indicating a high degree of collagen exposure for interaction with other ECM proteins or cells contacting the biomaterial surface. Much reduced collagen exposure (around 50%) was measured for the 1:20 collagen:PCL materials. These findings were consistent with the SEM examination of collagen:PCL biocomposites which revealed a highly porous morphology for the 1:4 and 1:8 blends but virtually complete coverage of the collagen component by PCL in the1:20 samples. Investigations of the attachment and spreading characteristics of human osteoblast (HOB) cells on PCL films and collagen:PCL materials respectively, indicated that HOB cells poorly recognised PCL but attachment and spreading were much improved on the biocomposites. The non-chemically crosslinked, collagen:PCL biocomposites described are expected to provide a useful addition to the range of biomaterials and matrix systems for tissue engineering.
Original languageEnglish
Pages (from-to)2113-2118
Number of pages6
JournalBiomaterials
Volume23
Issue number10
DOIs
Publication statusPublished - May 2002

Fingerprint

Collagen
Polymers
Osteoblasts
Biocompatible Materials
Biomaterials
Digestion
caprolactone
Natural polymers
Military electronic countermeasures
Methylene Chloride
Dichloromethane
Collagenases
Tissue Engineering
Tissue engineering
Impregnation
Crosslinking
Toxicity
Evaporation
Proteins
Weights and Measures

Keywords

  • collagen
  • polycaprolactone
  • bone cells
  • biocomposite

Cite this

Coombes, A. G. A., Verderio, E., Shaw, B., Li, X., Griffin, M., & Downes, S. (2002). Biocomposites of non-crosslinked natural and synthetic polymers. Biomaterials, 23(10), 2113-2118. https://doi.org/10.1016/S0142-9612(01)00341-6
Coombes, A.G.A. ; Verderio, E. ; Shaw, B. ; Li, Xiaoling ; Griffin, Martin ; Downes, S. / Biocomposites of non-crosslinked natural and synthetic polymers. In: Biomaterials. 2002 ; Vol. 23, No. 10. pp. 2113-2118.
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Coombes, AGA, Verderio, E, Shaw, B, Li, X, Griffin, M & Downes, S 2002, 'Biocomposites of non-crosslinked natural and synthetic polymers', Biomaterials, vol. 23, no. 10, pp. 2113-2118. https://doi.org/10.1016/S0142-9612(01)00341-6

Biocomposites of non-crosslinked natural and synthetic polymers. / Coombes, A.G.A.; Verderio, E.; Shaw, B.; Li, Xiaoling; Griffin, Martin; Downes, S.

In: Biomaterials, Vol. 23, No. 10, 05.2002, p. 2113-2118.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biocomposites of non-crosslinked natural and synthetic polymers

AU - Coombes, A.G.A.

AU - Verderio, E.

AU - Shaw, B.

AU - Li, Xiaoling

AU - Griffin, Martin

AU - Downes, S.

PY - 2002/5

Y1 - 2002/5

N2 - Biocomposite films comprising a non-crosslinked, natural polymer (collagen) and a synthetic polymer, poly(var epsilon-caprolactone) (PCL), have been produced by impregnation of lyophilised collagen mats with a solution of PCL in dichloromethane followed by solvent evaporation. This approach avoids the toxicity problems associated with chemical crosslinking. Distinct changes in film morphology, from continuous surface coating to open porous format, were achieved by variation of processing parameters such as collagen:PCL ratio and the weight of the starting lyophilised collagen mat. Collagenase digestion indicated that the collagen content of 1:4 and 1:8 collagen:PCL biocomposites was almost totally accessible for enzymatic digestion indicating a high degree of collagen exposure for interaction with other ECM proteins or cells contacting the biomaterial surface. Much reduced collagen exposure (around 50%) was measured for the 1:20 collagen:PCL materials. These findings were consistent with the SEM examination of collagen:PCL biocomposites which revealed a highly porous morphology for the 1:4 and 1:8 blends but virtually complete coverage of the collagen component by PCL in the1:20 samples. Investigations of the attachment and spreading characteristics of human osteoblast (HOB) cells on PCL films and collagen:PCL materials respectively, indicated that HOB cells poorly recognised PCL but attachment and spreading were much improved on the biocomposites. The non-chemically crosslinked, collagen:PCL biocomposites described are expected to provide a useful addition to the range of biomaterials and matrix systems for tissue engineering.

AB - Biocomposite films comprising a non-crosslinked, natural polymer (collagen) and a synthetic polymer, poly(var epsilon-caprolactone) (PCL), have been produced by impregnation of lyophilised collagen mats with a solution of PCL in dichloromethane followed by solvent evaporation. This approach avoids the toxicity problems associated with chemical crosslinking. Distinct changes in film morphology, from continuous surface coating to open porous format, were achieved by variation of processing parameters such as collagen:PCL ratio and the weight of the starting lyophilised collagen mat. Collagenase digestion indicated that the collagen content of 1:4 and 1:8 collagen:PCL biocomposites was almost totally accessible for enzymatic digestion indicating a high degree of collagen exposure for interaction with other ECM proteins or cells contacting the biomaterial surface. Much reduced collagen exposure (around 50%) was measured for the 1:20 collagen:PCL materials. These findings were consistent with the SEM examination of collagen:PCL biocomposites which revealed a highly porous morphology for the 1:4 and 1:8 blends but virtually complete coverage of the collagen component by PCL in the1:20 samples. Investigations of the attachment and spreading characteristics of human osteoblast (HOB) cells on PCL films and collagen:PCL materials respectively, indicated that HOB cells poorly recognised PCL but attachment and spreading were much improved on the biocomposites. The non-chemically crosslinked, collagen:PCL biocomposites described are expected to provide a useful addition to the range of biomaterials and matrix systems for tissue engineering.

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KW - polycaprolactone

KW - bone cells

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