Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Yana Tarakanchikova; Jamal Alzubi; Valentina Pennucci; Marie Follo; Boris Kochergin; Albert Muslimov; Ilya Skovorodkin; Seppo Vainio; Maria N. Antipina; Vsevolod Atkin; Alexey Popov; Igor Meglinski; Toni Cathomen; Tatjana I. Cornu; Dmitry A. Gorin; Gleb B. Sukhorukov; Irina Nazarenko

doi:10.1002/smll.v16.3

Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Yana Tarakanchikova, Jamal Alzubi, Valentina Pennucci, Marie Follo, Boris Kochergin, Albert Muslimov, Ilya Skovorodkin, Seppo Vainio, Maria N. Antipina, Vsevolod Atkin, Alexey Popov, Igor Meglinski, Toni Cathomen, Tatjana I. Cornu, Dmitry A. Gorin, Gleb B. Sukhorukov, Irina Nazarenko

Mechanical, Biomedical & Design Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

Original language	English
Article number	1904880
Journal	small
Volume	16
Issue number	3
Early online date	16 Dec 2019
DOIs	https://doi.org/10.1002/smll.v16.3
Publication status	Published - 20 Jan 2020

Bibliographical note

© 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Keywords

biomimetics
drug delivery
extracellular vesicles
gene delivery
microcapsules
nanocapsules

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10.1002/smll.v16.3Licence: CC BY 3.0

Biodegradable Nanocarriers Resembling Extracellular Vesicles
© 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.64 MBLicence: CC BY 3.0

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Tarakanchikova, Y., Alzubi, J., Pennucci, V., Follo, M., Kochergin, B., Muslimov, A., Skovorodkin, I., Vainio, S., Antipina, M. N., Atkin, V., Popov, A., Meglinski, I., Cathomen, T., Cornu, T. I., Gorin, D. A., Sukhorukov, G. B., & Nazarenko, I. (2020). Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types. small, 16(3), Article 1904880. https://doi.org/10.1002/smll.v16.3

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title = "Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types",

abstract = "Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.",

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Tarakanchikova, Y, Alzubi, J, Pennucci, V, Follo, M, Kochergin, B, Muslimov, A, Skovorodkin, I, Vainio, S, Antipina, MN, Atkin, V, Popov, A, Meglinski, I, Cathomen, T, Cornu, TI, Gorin, DA, Sukhorukov, GB & Nazarenko, I 2020, 'Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types', small, vol. 16, no. 3, 1904880. https://doi.org/10.1002/smll.v16.3

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T1 - Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

AU - Tarakanchikova, Yana

AU - Alzubi, Jamal

AU - Pennucci, Valentina

AU - Follo, Marie

AU - Kochergin, Boris

AU - Muslimov, Albert

AU - Skovorodkin, Ilya

AU - Vainio, Seppo

AU - Antipina, Maria N.

AU - Atkin, Vsevolod

AU - Popov, Alexey

AU - Meglinski, Igor

AU - Cathomen, Toni

AU - Cornu, Tatjana I.

AU - Gorin, Dmitry A.

AU - Sukhorukov, Gleb B.

AU - Nazarenko, Irina

N1 - © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PY - 2020/1/20

Y1 - 2020/1/20

N2 - Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

AB - Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

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KW - drug delivery

KW - extracellular vesicles

KW - gene delivery

KW - microcapsules

KW - nanocapsules

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DO - 10.1002/smll.v16.3

M3 - Article

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VL - 16

JO - small

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IS - 3

M1 - 1904880

ER -

Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Abstract

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Keywords

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