Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Yana Tarakanchikova; Jamal Alzubi; Valentina Pennucci; Marie Follo; Boris Kochergin; Albert Muslimov; Ilya Skovorodkin; Seppo Vainio; Maria N. Antipina; Vsevolod Atkin; Alexey Popov; Igor Meglinski; Toni Cathomen; Tatjana I. Cornu; Dmitry A. Gorin; Gleb B. Sukhorukov; Irina Nazarenko

doi:10.1002/smll.v16.3

Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Yana Tarakanchikova
, Jamal Alzubi
, Valentina Pennucci
, Marie Follo
, Boris Kochergin
, Albert Muslimov
, Ilya Skovorodkin
, Seppo Vainio
, Maria N. Antipina
, Vsevolod Atkin
, Alexey Popov
, Igor Meglinski
, Toni Cathomen
, Tatjana I. Cornu
, Dmitry A. Gorin
, Gleb B. Sukhorukov
, Irina Nazarenko

RASA center in St. PetersburgPeter the Great St. Petersburg Polytechnic University St. Petersburg 195251 Russia
Center for Chronic ImmunodeficiencyMedical Center–University of FreiburgFaculty of MedicineUniversity of Freiburg 79106 Freiburg Germany
Department of Medicine IMedical Center–University of FreiburgFaculty of MedicineUniversity of Freiburg Freiburg 153000 Germany
Department of Inorganic ChemistryIvanovo State University of Chemistry and Technology Sheremetievskiy Avenue 7 153000 Ivanovo Russia
Nanobiotechnology LaboratorySt. Petersburg Academic University St. Petersburg 194021 Russia
Faculty of Biochemistry and Molecular MedicineBiocenter OuluLaboratory of Developmental BiologyInfotech OuluUniversity of Oulu Borealis Biobank of Northern Finland 138634 Oulu Finland
Institute of Materials Research and EngineeringA*STAR Singapore 138634 Singapore
Educational Research Institute of Nanostructures and BiosystemsSaratov State University Saratov, Astrakhanskaya 83 410012 Saratov Russia
Opto‐Electronics and Measurement Techniques Research UnitP.O. Box 4500University of Oulu Oulu 90014 Finland
Skoltech center of Photonics & Quantum MaterialsSkolkovo Institute of Science and TechnologySkolkovo Innovation Center Building 3 Moscow 143026 Russia
School of Engineering and Material ScienceQueen Mary University of London London B47ET UK
German Cancer Consortium (DKTK)Partner Site Freiburg and German Cancer Research Center (DKFZ) Heidelberg B47ET Germany

Research output: Contribution to journal › Article › peer-review

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Abstract

Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

Original language	English
Article number	1904880
Journal	small
Volume	16
Issue number	3
Early online date	16 Dec 2019
DOIs	https://doi.org/10.1002/smll.v16.3
Publication status	Published - 20 Jan 2020

Bibliographical note

© 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Keywords

biomimetics
drug delivery
extracellular vesicles
gene delivery
microcapsules
nanocapsules

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10.1002/smll.v16.3Licence: CC BY 3.0

Biodegradable Nanocarriers Resembling Extracellular Vesicles
© 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.64 MBLicence: CC BY 3.0

Cite this

Tarakanchikova, Y., Alzubi, J., Pennucci, V., Follo, M., Kochergin, B., Muslimov, A., Skovorodkin, I., Vainio, S., Antipina, M. N., Atkin, V., Popov, A., Meglinski, I., Cathomen, T., Cornu, T. I., Gorin, D. A., Sukhorukov, G. B., & Nazarenko, I. (2020). Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types. small, 16(3), Article 1904880. https://doi.org/10.1002/smll.v16.3

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title = "Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types",

abstract = "Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100\% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.",

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author = "Yana Tarakanchikova and Jamal Alzubi and Valentina Pennucci and Marie Follo and Boris Kochergin and Albert Muslimov and Ilya Skovorodkin and Seppo Vainio and Antipina, \{Maria N.\} and Vsevolod Atkin and Alexey Popov and Igor Meglinski and Toni Cathomen and Cornu, \{Tatjana I.\} and Gorin, \{Dmitry A.\} and Sukhorukov, \{Gleb B.\} and Irina Nazarenko",

note = "{\textcopyright} 2019 The Authors. Published by WILEY‐VCH Verlag GmbH \& Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.",

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Tarakanchikova, Y, Alzubi, J, Pennucci, V, Follo, M, Kochergin, B, Muslimov, A, Skovorodkin, I, Vainio, S, Antipina, MN, Atkin, V, Popov, A, Meglinski, I, Cathomen, T, Cornu, TI, Gorin, DA, Sukhorukov, GB & Nazarenko, I 2020, 'Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types', small, vol. 16, no. 3, 1904880. https://doi.org/10.1002/smll.v16.3

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T1 - Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

AU - Tarakanchikova, Yana

AU - Alzubi, Jamal

AU - Pennucci, Valentina

AU - Follo, Marie

AU - Kochergin, Boris

AU - Muslimov, Albert

AU - Skovorodkin, Ilya

AU - Vainio, Seppo

AU - Antipina, Maria N.

AU - Atkin, Vsevolod

AU - Popov, Alexey

AU - Meglinski, Igor

AU - Cathomen, Toni

AU - Cornu, Tatjana I.

AU - Gorin, Dmitry A.

AU - Sukhorukov, Gleb B.

AU - Nazarenko, Irina

N1 - © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PY - 2020/1/20

Y1 - 2020/1/20

N2 - Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

AB - Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

KW - biomimetics

KW - drug delivery

KW - extracellular vesicles

KW - gene delivery

KW - microcapsules

KW - nanocapsules

UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.201904880

UR - https://www.scopus.com/pages/publications/85076792837

U2 - 10.1002/smll.v16.3

DO - 10.1002/smll.v16.3

M3 - Article

SN - 1613-6810

VL - 16

JO - small

JF - small

IS - 3

M1 - 1904880

ER -

Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Abstract

Bibliographical note

Keywords

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