Biophotonics approach for the study of leukocyte activation

Daria N. Chernova; Sergei G. Sokolovski; Alexey Yu Vorobev; Alexander E. Moskalensky

doi:10.1117/12.2560325

Biophotonics approach for the study of leukocyte activation

Daria N. Chernova, Sergei G. Sokolovski, Alexey Yu Vorobev, Alexander E. Moskalensky

Aston Institute of Photonic Technologies (AiPT)

Research output: Chapter in Book/Published conference output › Conference publication

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Abstract

Leukocytes are the main cells of immune system, but also contribute to other systems and participate in pathogenesis of different diseases. In particular, leukocytes are involved in the progression of diabetic retinopathy due to their hyperactivation in diabetes. However, a connection between diabetes and the dysfunction of leukocytes is poorly understood. For a more complete picture, studies of the leukocytes activation under the influence of various substances are necessary. Arachidonic acid (AA) and its metabolites are the strongest activating factors of leukocytes. However, the studies involving AA are complicated because it is water-insoluble. Here we describe the method to study activation using photolabile analogs of AA.

Original language	English
Title of host publication	Saratov Fall Meeting 2019
Subtitle of host publication	Optical and Nano-Technologies for Biology and Medicine
Editors	Valery V. Tuchin, Valery V. Tuchin, Elina A. Genina
Publisher	Society of Photo-Optical Instrumentation Engineers (SPIE)
ISBN (Electronic)	9781510637184
DOIs	https://doi.org/10.1117/12.2560325
Publication status	Published - 9 Apr 2020
Event	7th International Symposium on Optics and Biophotonics: Optical and Nano-Technologies for Biology and Medicine, SFM 2019 - Saratov, Russian Federation Duration: 23 Sept 2019 → 27 Sept 2019

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11457
ISSN (Print)	1605-7422

Conference

Conference	7th International Symposium on Optics and Biophotonics: Optical and Nano-Technologies for Biology and Medicine, SFM 2019
Country/Territory	Russian Federation
City	Saratov
Period	23/09/19 → 27/09/19

Bibliographical note

Copyright 2020 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Funding

This work was supported by the Grant of President of Russian Federation for state support of young scientists (Grant agreement 075-15-2019-1034 (МК-1715.2018.2)).

Keywords

Arachidonic acid
Biophotonics
Caged arachidonic acid
Dysfunction of leukocytes
Intracellular calcium
Jurkat cell
Photolabile analogs
Single cell optical control

Access to Document

10.1117/12.2560325

Biophotonics approach for the study of leukocyte activation
Copyright 2020 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Final published version, 381 KB

Cite this

Chernova, D. N., Sokolovski, S. G., Vorobev, A. Y., & Moskalensky, A. E. (2020). Biophotonics approach for the study of leukocyte activation. In V. V. Tuchin, V. V. Tuchin, & E. A. Genina (Eds.), Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine Article 114570G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11457). Society of Photo-Optical Instrumentation Engineers (SPIE). https://doi.org/10.1117/12.2560325

Chernova, Daria N. ; Sokolovski, Sergei G. ; Vorobev, Alexey Yu et al. / Biophotonics approach for the study of leukocyte activation. Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine. editor / Valery V. Tuchin ; Valery V. Tuchin ; Elina A. Genina. Society of Photo-Optical Instrumentation Engineers (SPIE), 2020. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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abstract = "Leukocytes are the main cells of immune system, but also contribute to other systems and participate in pathogenesis of different diseases. In particular, leukocytes are involved in the progression of diabetic retinopathy due to their hyperactivation in diabetes. However, a connection between diabetes and the dysfunction of leukocytes is poorly understood. For a more complete picture, studies of the leukocytes activation under the influence of various substances are necessary. Arachidonic acid (AA) and its metabolites are the strongest activating factors of leukocytes. However, the studies involving AA are complicated because it is water-insoluble. Here we describe the method to study activation using photolabile analogs of AA.",

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Chernova, DN, Sokolovski, SG, Vorobev, AY & Moskalensky, AE 2020, Biophotonics approach for the study of leukocyte activation. in VV Tuchin, VV Tuchin & EA Genina (eds), Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine., 114570G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11457, Society of Photo-Optical Instrumentation Engineers (SPIE), 7th International Symposium on Optics and Biophotonics: Optical and Nano-Technologies for Biology and Medicine, SFM 2019, Saratov, Russian Federation, 23/09/19. https://doi.org/10.1117/12.2560325

Biophotonics approach for the study of leukocyte activation. / Chernova, Daria N.; Sokolovski, Sergei G.; Vorobev, Alexey Yu et al.
Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine. ed. / Valery V. Tuchin; Valery V. Tuchin; Elina A. Genina. Society of Photo-Optical Instrumentation Engineers (SPIE), 2020. 114570G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11457).

Research output: Chapter in Book/Published conference output › Conference publication

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AU - Chernova, Daria N.

AU - Sokolovski, Sergei G.

AU - Vorobev, Alexey Yu

AU - Moskalensky, Alexander E.

N1 - Copyright 2020 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

PY - 2020/4/9

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N2 - Leukocytes are the main cells of immune system, but also contribute to other systems and participate in pathogenesis of different diseases. In particular, leukocytes are involved in the progression of diabetic retinopathy due to their hyperactivation in diabetes. However, a connection between diabetes and the dysfunction of leukocytes is poorly understood. For a more complete picture, studies of the leukocytes activation under the influence of various substances are necessary. Arachidonic acid (AA) and its metabolites are the strongest activating factors of leukocytes. However, the studies involving AA are complicated because it is water-insoluble. Here we describe the method to study activation using photolabile analogs of AA.

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Chernova DN, Sokolovski SG, Vorobev AY, Moskalensky AE. Biophotonics approach for the study of leukocyte activation. In Tuchin VV, Tuchin VV, Genina EA, editors, Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine. Society of Photo-Optical Instrumentation Engineers (SPIE). 2020. 114570G. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2560325

Biophotonics approach for the study of leukocyte activation

Abstract

Publication series

Conference

Bibliographical note

Funding

Keywords

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