Biophysical analysis of lipidic nanoparticles

Annaïg J. Rozo; Megan H. Cox; Andrew Devitt; Alice J. Rothnie; Alan D. Goddard

doi:10.1016/j.ymeth.2020.05.001

Biophysical analysis of lipidic nanoparticles

Annaïg J. Rozo, Megan H. Cox, Andrew Devitt, Alice J. Rothnie, Alan D. Goddard^*

^*Corresponding author for this work

School of Biosciences

Research output: Contribution to journal › Review article › peer-review

Abstract

Biological nanoparticles include liposomes, extracellular vesicle and lipid-based discoidal systems. When studying such particles, there are several key parameters of interest, including particle size and concentration. Measuring these characteristics can be of particular importance in the research laboratory or when producing such particles as biotherapeutics. This article briefly describes the major types of lipid-containing nanoparticles and the techniques that can be used to study them. Such methodologies include electron microscopy, atomic force microscopy, dynamic light scattering, nanoparticle tracking analysis, flow cytometry, tunable resistive pulse sensing and microfluidic resistive pulse sensing. Whilst no technique is perfect for the analysis of all nanoparticles, this article provides advantages and disadvantages of each, highlighting the latest developments in the field. Finally, we demonstrate the use of microfluidic resistive pulse sensing for the analysis of biological nanoparticles.

Original language	English
Pages (from-to)	45-55
Number of pages	11
Journal	Methods
Volume	180
Early online date	5 May 2020
DOIs	https://doi.org/10.1016/j.ymeth.2020.05.001
Publication status	Published - 1 Aug 2020

Bibliographical note

© 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Funding: We are grateful for funding from the European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No 847419 (MemTrain) and from Innovate UK in the form of a Knowledge Transfer Partnership between Aston University and Meritics Ltd (KTP 11605).

Keywords

Extracellular vesicles
Liposomes
MPRS
Nanoparticle
nCS1

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10.1016/j.ymeth.2020.05.001

Biophysical analysis of lipidic nanoparticles
© 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Accepted author manuscript, 913 KBLicence: CC BY-NC-ND 3.0

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title = "Biophysical analysis of lipidic nanoparticles",

abstract = "Biological nanoparticles include liposomes, extracellular vesicle and lipid-based discoidal systems. When studying such particles, there are several key parameters of interest, including particle size and concentration. Measuring these characteristics can be of particular importance in the research laboratory or when producing such particles as biotherapeutics. This article briefly describes the major types of lipid-containing nanoparticles and the techniques that can be used to study them. Such methodologies include electron microscopy, atomic force microscopy, dynamic light scattering, nanoparticle tracking analysis, flow cytometry, tunable resistive pulse sensing and microfluidic resistive pulse sensing. Whilst no technique is perfect for the analysis of all nanoparticles, this article provides advantages and disadvantages of each, highlighting the latest developments in the field. Finally, we demonstrate the use of microfluidic resistive pulse sensing for the analysis of biological nanoparticles.",

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note = "{\textcopyright} 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Funding: We are grateful for funding from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No 847419 (MemTrain) and from Innovate UK in the form of a Knowledge Transfer Partnership between Aston University and Meritics Ltd (KTP 11605).",

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doi = "10.1016/j.ymeth.2020.05.001",

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AU - Rozo, Annaïg J.

AU - Cox, Megan H.

AU - Devitt, Andrew

AU - Rothnie, Alice J.

AU - Goddard, Alan D.

N1 - © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Funding: We are grateful for funding from the European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No 847419 (MemTrain) and from Innovate UK in the form of a Knowledge Transfer Partnership between Aston University and Meritics Ltd (KTP 11605).

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N2 - Biological nanoparticles include liposomes, extracellular vesicle and lipid-based discoidal systems. When studying such particles, there are several key parameters of interest, including particle size and concentration. Measuring these characteristics can be of particular importance in the research laboratory or when producing such particles as biotherapeutics. This article briefly describes the major types of lipid-containing nanoparticles and the techniques that can be used to study them. Such methodologies include electron microscopy, atomic force microscopy, dynamic light scattering, nanoparticle tracking analysis, flow cytometry, tunable resistive pulse sensing and microfluidic resistive pulse sensing. Whilst no technique is perfect for the analysis of all nanoparticles, this article provides advantages and disadvantages of each, highlighting the latest developments in the field. Finally, we demonstrate the use of microfluidic resistive pulse sensing for the analysis of biological nanoparticles.

AB - Biological nanoparticles include liposomes, extracellular vesicle and lipid-based discoidal systems. When studying such particles, there are several key parameters of interest, including particle size and concentration. Measuring these characteristics can be of particular importance in the research laboratory or when producing such particles as biotherapeutics. This article briefly describes the major types of lipid-containing nanoparticles and the techniques that can be used to study them. Such methodologies include electron microscopy, atomic force microscopy, dynamic light scattering, nanoparticle tracking analysis, flow cytometry, tunable resistive pulse sensing and microfluidic resistive pulse sensing. Whilst no technique is perfect for the analysis of all nanoparticles, this article provides advantages and disadvantages of each, highlighting the latest developments in the field. Finally, we demonstrate the use of microfluidic resistive pulse sensing for the analysis of biological nanoparticles.

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JO - Methods

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Biophysical analysis of lipidic nanoparticles

Abstract

Bibliographical note

Keywords

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