Bioprocess forces and their impact on cell behavior: implications for bone regeneration therapy

David Brindley, Kishaani Moorthy, Jae-Ho Lee, Chris Mason, Hae-Won Kim, Ivan Wall

Research output: Contribution to journalArticle

Abstract

Bioprocess forces such as shear stress experienced during routine cell culture are considered to be harmful to cells. However, the impact of physical forces on cell behavior is an area of growing interest within the tissue engineering community, and it is widely acknowledged that mechanical stimulation including shear stress can enhance osteogenic differentiation. This paper considers the effects of bioprocess shear stress on cell responses such as survival and proliferation in several contexts, including suspension-adapted cells used for recombinant protein and monoclonal antibody manufacture, adherent cells for therapy in suspension, and adherent cells attached to their growth substrates. The enhanced osteogenic differentiation that fluid flow shear stress is widely found to induce is discussed, along with the tissue engineering of mineralized tissue using perfusion bioreactors. Recent evidence that bioprocess forces produced during capillary transfer or pipetting of cell suspensions can enhance osteogenic responses is also discussed.

Original languageEnglish
Article number620247
JournalJournal of Tissue Engineering
Volume2011
DOIs
Publication statusPublished - 2011

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Bone Regeneration
Shear stress
Bone
Suspensions
Tissue engineering
Tissue Engineering
Recombinant proteins
Monoclonal antibodies
Therapeutics
Bioreactors
Cell culture
Recombinant Proteins
Flow of fluids
Monoclonal Antibodies
Tissue
Cell- and Tissue-Based Therapy
Substrates
Cell Culture Techniques
Perfusion
Growth

Bibliographical note

Copyright © 2011 David Brindley et al. This is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this

Brindley, D., Moorthy, K., Lee, J-H., Mason, C., Kim, H-W., & Wall, I. (2011). Bioprocess forces and their impact on cell behavior: implications for bone regeneration therapy. Journal of Tissue Engineering, 2011, [620247]. https://doi.org/10.4061/2011/620247
Brindley, David ; Moorthy, Kishaani ; Lee, Jae-Ho ; Mason, Chris ; Kim, Hae-Won ; Wall, Ivan. / Bioprocess forces and their impact on cell behavior : implications for bone regeneration therapy. In: Journal of Tissue Engineering. 2011 ; Vol. 2011.
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Brindley, D, Moorthy, K, Lee, J-H, Mason, C, Kim, H-W & Wall, I 2011, 'Bioprocess forces and their impact on cell behavior: implications for bone regeneration therapy', Journal of Tissue Engineering, vol. 2011, 620247. https://doi.org/10.4061/2011/620247

Bioprocess forces and their impact on cell behavior : implications for bone regeneration therapy. / Brindley, David; Moorthy, Kishaani; Lee, Jae-Ho; Mason, Chris; Kim, Hae-Won; Wall, Ivan.

In: Journal of Tissue Engineering, Vol. 2011, 620247, 2011.

Research output: Contribution to journalArticle

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AU - Moorthy, Kishaani

AU - Lee, Jae-Ho

AU - Mason, Chris

AU - Kim, Hae-Won

AU - Wall, Ivan

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Brindley D, Moorthy K, Lee J-H, Mason C, Kim H-W, Wall I. Bioprocess forces and their impact on cell behavior: implications for bone regeneration therapy. Journal of Tissue Engineering. 2011;2011. 620247. https://doi.org/10.4061/2011/620247