Biosensor based on excessively tilted fiber grating in thin-cladding optical fiber for sensitive and selective detection of low glucose concentration

Binbin Luo, Zhijun Yan, Zhongyuan Sun, Yong Liu, Mingfu Zhao, Lin Zhang

Research output: Contribution to journalArticle

Abstract

We report a highly sensitive, high Q-factor, label free and selective glucose sensor by using excessively tilted fiber grating (Ex-TFG) inscribed in the thin-cladding optical fiber (TCOF). Glucose oxidase (GOD) was covalently immobilized on optical fiber surface and the effectiveness of GOD immobilization was investigated by the fluorescence microscopy and highly accurate spectral interrogation method. In contrast to the long period grating (LPG) and optical fiber (OF) surface Plasmon resonance (SPR) based glucose sensors, the Ex-TFG configuration has merits of nearly independent cross sensitivity of the environmental temperature, simple fabrication method (no noble metal deposition or cladding etching) and high detection accuracy (or Q-factor). Our experimental results have shown that Ex-TFG in TCOF based sensor has a reliable and fast detection for the glucose concentration as low as 0.1~2.5mg/ml and a high sensitivity of ~1.514nm·(mg/ml)−1, which the detection accuracy is ~0.2857nm−1 at pH 5.2, and the limit of detection (LOD) is 0.013~0.02mg/ml at the pH range of 5.2~7.4 by using an optical spectrum analyzer with a resolution of 0.02nm.
Original languageEnglish
Pages (from-to)32429-32440
Number of pages12
JournalOptics Express
Volume23
Issue number25
DOIs
Publication statusPublished - 9 Dec 2015

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bioinstrumentation
glucose
optical fibers
gratings
fibers
Q factors
oxidase
sensors
sensitivity
interrogation
noble metals
immobilization
surface plasmon resonance
ambient temperature
optical spectrum
analyzers
etching
microscopy
fluorescence
fabrication

Cite this

Luo, Binbin ; Yan, Zhijun ; Sun, Zhongyuan ; Liu, Yong ; Zhao, Mingfu ; Zhang, Lin. / Biosensor based on excessively tilted fiber grating in thin-cladding optical fiber for sensitive and selective detection of low glucose concentration. In: Optics Express. 2015 ; Vol. 23, No. 25. pp. 32429-32440.
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abstract = "We report a highly sensitive, high Q-factor, label free and selective glucose sensor by using excessively tilted fiber grating (Ex-TFG) inscribed in the thin-cladding optical fiber (TCOF). Glucose oxidase (GOD) was covalently immobilized on optical fiber surface and the effectiveness of GOD immobilization was investigated by the fluorescence microscopy and highly accurate spectral interrogation method. In contrast to the long period grating (LPG) and optical fiber (OF) surface Plasmon resonance (SPR) based glucose sensors, the Ex-TFG configuration has merits of nearly independent cross sensitivity of the environmental temperature, simple fabrication method (no noble metal deposition or cladding etching) and high detection accuracy (or Q-factor). Our experimental results have shown that Ex-TFG in TCOF based sensor has a reliable and fast detection for the glucose concentration as low as 0.1~2.5mg/ml and a high sensitivity of ~1.514nm·(mg/ml)−1, which the detection accuracy is ~0.2857nm−1 at pH 5.2, and the limit of detection (LOD) is 0.013~0.02mg/ml at the pH range of 5.2~7.4 by using an optical spectrum analyzer with a resolution of 0.02nm.",
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Biosensor based on excessively tilted fiber grating in thin-cladding optical fiber for sensitive and selective detection of low glucose concentration. / Luo, Binbin; Yan, Zhijun; Sun, Zhongyuan; Liu, Yong; Zhao, Mingfu; Zhang, Lin.

In: Optics Express, Vol. 23, No. 25, 09.12.2015, p. 32429-32440.

Research output: Contribution to journalArticle

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