Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A

Karina T. Wright, Wagih El Masri, Aheed Osman, Sally Roberts, Giselle Chamberlain, Brian A. Ashton, William E.B. Johnson

Research output: Contribution to journalArticle

Abstract

In animal models, transplantation of bone marrow stromal cells (MSC) into the spinal cord following injury enhances axonal regeneration and promotes functional recovery. How these improvements come about is currently unclear. We have examined the interaction of MSC with neurons, using an established in vitro model of nerve growth, in the presence of substrate-bound extracellular molecules that are thought to inhibit axonal regeneration, i.e., neural proteoglycans (CSPG), myelin associated glycoprotein (MAG) and Nogo-A. Each of these molecules repelled neurite outgrowth from dorsal root ganglia (DRG) in a concentration-dependent manner. However, these nerve-inhibitory effects were much reduced in MSC/DRG co-cultures. Video microscopy demonstrated that MSC acted as "cellular bridges" and also "towed" neurites over the nerve-inhibitory substrates. Whereas conditioned medium from MSC cultures stimulated DRG neurite outgrowth over type I collagen, it did not promote outgrowth over CSPG, MAG or Nogo-A. These findings suggest that MSC transplantation may promote axonal regeneration both by stimulating nerve growth via secreted factors and also by reducing the nerve-inhibitory effects of the extracellular molecules present.
Original languageEnglish
Pages (from-to)559-566
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume354
Issue number2
Early online date10 Jan 2007
DOIs
Publication statusPublished - 9 Mar 2007

Keywords

  • stromal cells
  • proteoglycans
  • bone marrow cells
  • cell enlargement
  • cultured cells
  • neurons
  • Myelin proteins
  • neurites
  • Myelin-associated glycoprotein

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